• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 2
  • Tagged with
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Chemical Characterisation of Compression Wood in Plantation Grown Pinus Radiata

Nanayakkara, Bernadette January 2007 (has links)
The primary objective of this study was to find out if changes in chemistry could be used to quantify Pinus radiata compression wood severity or degree of compression wood development. Basic chemical composition and the lignin structure was assessed for a range of different compression wood samples sourced from juvenile wood, mature wood, earlywood, latewood, branches, knots, 2-year and 1-year old Pinus radiata. Fluorescence microscopy was used as the reference method to assess the degree of compression wood development. Lignin structure of compression wood was studied by thioacidolysis, size exclusion chromatography, and thioacidolysis/31P NMR spectroscopy. Variation in the basic chemical composition and lignin structure with compression wood severity was ascertained. Results showed that, as the severity of compression wood changed, progressively from normal through mild to severe, all chemical parameters commonly associated with compression wood changed concurrently. With increasing severity lignin and galactose levels increased while glucose and mannose levels decreased. Lignin structural changes were also associated with changing severity of compression wood. Levels of p-hydroxyphenyl (H) releasable β-ethers increased and guaiacyl (G) releasable β-ethers decreased. Similarly, levels of uncondensed p-hydroxyphenyl units increased, while uncondensed guaiacyl units decreased. Similar proportions of condensed guaiacyl units were present in compression wood and normal wood. Similar trends in chemical composition were observed between the compression wood and related opposite wood in branches, knots and young wood of Pinus radiata. A number of chemical parameters changed linearly with compression wood severity. They were: the amount of lignin and galactose, the galactose/glucose ratio and p-hydroxyphenyl content in lignin. Parameters based on the p-hydroxyphenyl unit content in lignin, the H/G releasable β-ether ratio, releasable p-hydroxyphenyl β-ether units and uncondensed p-hydroxyphenyl C9 units are most suitable indicators of compression wood severity as they spanned a larger range relative to the normal wood levels and were not influenced by the morphological origin of wood samples. Chemical methods for quantifying compression wood severity should focus on the detection and measurement of these parameters. Galactan present in Pinus radiata compression wood was isolated and characterised. Structural investigation by methylation analysis and NMR spectroscopy revealed that this galactan was largely composed of (1→4)-linked β-D-galactopyranose residues. No evidence was found to indicate the presence of any branches. Characterisation of lignin in cell wall fractions of Pinus radiata normal wood revealed that middle lamella lignin has a higher lignin content, a lower amount of releasable β-ethers and a more condensed lignin than the secondary wall lignin. Levels of releasable p-hydroxyphenyl units were not higher in middle lamella lignin. A new method based on thioacidolysis and 31P quantitative NMR spectroscopy for estimation of the degree of lignin condensation of the phenolic and etherified C9 units in in situ wood lignin is described. Using this method it was found that phenolic C9 units in in situ lignin were considerably less condensed than etherified C9 units in both compression wood and normal wood.
2

APPLICATIONS OF GAS CHROMATOGRAPHY/MASS SPECTROMETRY AND CAPILLARY ELECTROPHORESIS FOR THE ANALYSIS OF LIGNOCELLULOSIC BIOMASS PRETREATMENT

Kato, Dawn M 01 January 2014 (has links)
The focus of this dissertation centers on the development and applications of gas chromatography/mass spectrometry and capillary electrophoresis methodologies to quantify monomeric compositions of the β-O-4 linkages in lignin. Pretreatment is a required step in the utilization of lignocellulosic biomass for biofuels. Lignin is the target of pretreatment because it hinders the accessibility of enzymes and chemicals to cellulose. The effects of pretreatment are commonly assessed utilizing enzymatic saccharification and lignin assays. However, these techniques do not elucidate the effects of pretreatment on the monomeric make up of lignin. The overarching hypothesis of this dissertation is that changes in individual monolignol content upon pretreatment can be observed from quantification. To test the hypothesis, a pretreatment, solution phase Fenton chemistry, was conducted on various lignocellulosic biomass feedstocks. Enzymatic saccharification studies showed a significant increase in glucose production upon Fenton pretreatment, however, lignin assays did not show a significant decrease in lignin content. Project two of this dissertation aimed to synthesize analytical standards in order to develop a quantitative thioacidolysis technique. The successful synthesis of the three arylglycerols were conducted utilizing and epoxidation reaction scheme which was hypothesized to produce a single diastereomer, as supported by GC/MS and chiral CE analysis. Upon method development, a quantitative thioacidolysis GC/MS method was applied to untreated and Fenton treated biomass. Results from this project revealed there was no significant change in the three lignin monomers. To verify the method, quantitative thioacidolysis GC/MS method was applied to a pretreatment method known to degrade lignin, alkaline peroxide pretreatment. The results of this project showed a significant change in monolignol concentrations upon alkaline peroxide pretreatment. Analytical degradative techniques, such as thioacidolysis, has traditionally assessed lignin as monomeric ratios. However, as this dissertation showed, upon alkaline peroxide pretreatment, no significant change was seen in the monomeric ratios, but there was a significant difference in all three monolignol concentrations. These results support the overall hypothesis that changes in individual monolignol content upon pretreatment can be observed from quantification. The works of this dissertation provides an analytical method which contributes to the elucidation of lignin.
3

Composition of lignin in outer cell-wall layers

Christiernin, Maria January 2006 (has links)
The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. Accurate data relating to the lignin monomer composition and the number of β-O-4´ bonds were obtained from pure middle lamella/primary cell wall lignin. Firstly, a 10 000 year old white spruce material, with most of the secondary cell wall missing, was studied. The aged lignin was composed of guaiacyl units only, and was slightly more condensed but otherwise similar to the reference lignin. Secondly, the developing xylem of a Norway spruce clone was analyzed during a growth season. In spring and early summer, growth is very rapid and the intention was to sample tissues in which the secondary cell-wall layers had not yet lignified, but where the outer layers at least had started to lignify. Microscopy, Klason lignin and carbohydrate analyses showed that the lignin in the developing xylem of samples from mid-June was located exclusively in the middle lamella. The lignin was more condensed, was composed of guaiacyl units only and contained more end-groups than the reference Norway spruce wood. Thirdly, the cambial tissues of a Balsam poplar clone were surveyed during a growth season. Both the phloem side and the xylem side of the cambial region were examined. The Klason lignin content and carbohydrate monomer distribution showed that in June and August the tissues on the phloem side contained material with mainly middle lamella/primary walls. In June, the xylem side in the cambial region contained mainly middle lamella/primary walls, and in August the secondary cell wall carbohydrates were being deposited. Both tissues contained lignin that was more condensed and had more end-groups than the reference lignin. In mid-June, the developing xylem had a ratio of syringyl to guaiacyl units of 0.6, whereas the ratio for the reference wood was 1.3. In the final study, lignin from the primary cell walls from a hybrid aspen cell suspension culture was investigated. The lignin contained only guaiacyl units which were more condensed than those observed in the reference poplar wood. / <p>QC 20100920</p>

Page generated in 0.0407 seconds