Wound treatment is a significant field in healthcare, but one with huge potential and need for advancement. Infection monitoring, in its current state, is a largely primitive affair, relying on visual and olfactory inspection to detect bacteria. As a result, early detection is impossible, and doctors and patients are forced to remove dressings to investigate the wound in a laborious, painful, and unsanitary process. When an infection is detected, the treatment is typically systemic administration of antibiotics. Systemic administration reduces the concentration of antibiotics that can be brought to bear on the infection because it interacts with the entire body and is dissipated by the time it reaches the wound and increases the risk of side effects or antibiotic resistance. Within this thesis, a smart, thread-based wound dressing is presented that addresses these issues by providing a pH-based early detection system accompanied by a topical, on-demand drug delivery system. The device has been tested in vitro and in vivo, on bacterial culture and on an animal model, and demonstrated effectiveness at detecting and eliminating bacteria, and at promoting wound healing. This smart wound dressing has the potential to improve treatment and outcomes for a wide variety of injuries, varying from burns to chronic wounds. / Graduate / 2020-10-10

Capillary electrophoresis chip has gradually ripe along with the development of MEMS technology. However, such these electrophoresis chips was design closed-channel form whose process including the micro-channel forming and chip bonding and so on, so the cost is higher. In addition, if these chips use repeated will cause some pollution problems such as obstruction or difficult to clean in the closed-channel. Therefore, to fabricate a non-closed microfluidic chip system will resolve the issues above listed. In this study has successful developed a convenient and low-cost thread microfluidic system, the thin polyester thread is instead of the traditional closed separation channel. And to avoid the cross contaminations that the separation channel can free replace a new electrophoresis separation channel by the roller equipment. Thread microfluidic systems can take advantage of capillary action to move, as a disposable chromatography flow channel, and the electric field is applied to this system for electrophoresis separation and electrochemical detection in the backend. This research develop an novel process technology, the hot embossing technology shape from concave embossing and metal coating procedure in PMMA, the salient pillow-electrode structure has be produced in PMMA board, the salient electrode structure set up the polyester fiber thread, the polyester fiber thread is as the electrophoresis separation channel, and electrochemical detect samples in back-end. In this study take plasma treatment to improve wettability and surface roughness of the polyester fiber thread, in order to improve the operational effectiveness of the thread microfluidic systems. The cyclic voltammetry measure potassium ferricyanide samples and the results showed that the performance of thread microfluidic system significantly increase after the plasma treatment, the measuring current value is 10 times greater than without the plasma treatment, and the estimated detection limit of potassium ferricyanide is around 6.25 £gM in the plasma treatment one. In addition, the thread microfluidic devices with plasma treatment has successful separation and detection the mixing samples of 0.3 mM chlorine, bromine and iodine ions, and the signal of the S/N ratio is 6 times higher than the without plasma treatment one, and the number of theoretical plates of electrophoresis separation also enhance to 28% in the plasma treatment one. In addition, in order to further enhance the detection sensitivity of the thread microfluidic systems, the study design and fabricate the concave-shaped three-dimensional electrode structure, and to achieve greater contact area between thread and the electrode. Thread microfluidic system with plasma treatment detect in concave-shaped three-dimensional electrode and flat electrode respectively, the results showed that concave-shaped electrodes in the potassium ferricyanide detection limit that measured current value is 10 times greater than flat electrode, and a mixture of dopamine and catechol sample are electrophoresis separated that concave-shaped 3D electrode whose measured S/N ratio is 5 times higher than flat electrode, and the number of theoretical plates is 1.5 times higher than the flat electrode. This study develops a novel thread microfluidic system that will provide a simple process and low-cost, and can solve the shortcomings of conventional closed-channel electrophoresis chips. In future, in this study develop the thread microfluidic system architecture will have contribute for fast electrophoresis separation and detection.

Šio magistrinio darbo tikslas yra teoriškai ir praktiškai išnagrinėti kaip ir kokius žinomus užklausų srautų aptarnavimo sprendimo būdus galima realizuoti JAVA programavimo kalbos priemonėmis. Darbo metu iškeltus uždavinius sudaro konkurentiškumą valdančių modelių išnagrinėjimas, jų realizavimo JAVA programavimo kalbos priemonėmis analizė, realiai veikiančių komponentų sumodeliavimas ir sukūrimas, sukurtų konkurentiškumą valdančių komponentų tyrimas, išvadų bei rekomendacijų pateikimas siekiant perteikti darbo metu įgytas žinias kaip galima parametrizuoti užklausų srautų aptarnavimo komponentą užtikrinant optimalų išteklių panaudojimo, pralaidumo bei uždelsimo balansą. Darbo metu buvo nagrinėjami dviejų tipų konkurentiškumą valdantys modeliai, t.y. gijos pagrindu bei du įvykių išskyrimo ir reagavimo pagrindu realizuoti modeliai, kur pastarieji du buvo suprojektuoti atsižvelgiant į Douglas Schmidto siūlomas reaktoriaus ir proaktoriaus projektavimo schemas. Išanalizavus JAVA programavimo kalbos galimybes, paaiškėjo, kad visus modelius galima kuo puikiausiai sumodeliuoti ir realizuoti panaudojant sinchroninį besiblokuojantį įvedimo ir išvedimo posistemį, sinchroninį nesiblokuojantį įvedimo ir išvedimo posistemį (NIO) ir asinchroninį įvedimo ir išvedimo posistemį (AIO4J). Minėtas aukščiau konkurentiškumą valdantis komponentas buvo realiai panaudotas ir išbandytas sukurtame supaprastintame HTTP serveryje, kaip komponentas atsakingas už ryšio užmezgimą bei užklausų apdorojimą... [toliau žr. visą tekstą] / The purpose of this master thesis is to analyze how many of known solutions for request stream handling can be implemented in JAVA programming language. Also, in addition to it, this master thesis analyzes the way those solutions can be implemented using JAVA programming language. There are defined tasks to investigate in the scope of this thesis, which include following: exploration of well known concurrency managing models; analysis of their implementation possibilities in JAVA by designing and creating real working components; their implementation workflow research under various stress loaded conditions and, finally, supply recommendations for optimal parameterization for mentioned component to gain the best possible balance of resource utilization, throughput and latency. There were analyzed basically two types of concurrency managing models in this work, i.e. thread based and two models based on event notification principle, designed using Douglas Schmidt reactor and proactor design patterns. During exploration of JAVA programming language possibilities to implement above mentioned models a special attention was paid to the new input/output system (NIO) which provides synchronous non-blocking input/output operations, whereas to process operations asynchronously there was explored asynchronous execution of input/output operations by using JAVA non-native external AIO4J library created by IBM corporation. In order to perform stress and performance benchmarks for created... [to full text]