by Judy, Po-shan Lai. / Thesis (M.Sc.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references(leaves 83-87). / Abbreviations --- p.iii / List of Tables --- p.iv / List of Figures --- p.v / Acknowledgements --- p.vi / SUMMARY --- p.1 / Chapter 1. --- INTRODUCTION --- p.2 / Chapter 2. --- BACKGROUND --- p.4 / Chapter 2.1 --- PROBLEMS ENCOUNTERED IN THYROID FUNCTION TESTS --- p.4 / Chapter 2.2 --- A NEED FOR TISSUE MARKER VERSUS EXISTING THYROID FUNCTION TESTS --- p.8 / Chapter 2.3 --- CHOICE OF TISSUE MARKER AS AN ADJUNCT TO THYROID FUNCTION TESTS --- p.10 / Chapter 2.3.1 --- ERYTHROCYTE ZINC --- p.10 / Chapter 2.3.2 --- ERYTHROCYTE CARBONIC ANHYDRASE ISOENZYME --- p.11 / Chapter 3. --- CARBONIC ANHYDRASE --- p.14 / Chapter 3.1 --- HISTORY AND BACKGROUND --- p.14 / Chapter 3.2 --- CARBONIC ANHYDRASE ISOENZYMES --- p.15 / Chapter 3.2.1 --- STRUCTURE AND DISTRIBUTION --- p.15 / Chapter 3.2.2 --- GENERAL MECHANISMS --- p.18 / Chapter 3.2.3 --- GENETICS AND REGULATION --- p.18 / Chapter 3.2.4 --- PHYSIOLOGICAL AND CLINICAL ASPECTS --- p.20 / Chapter 4. --- ANALYTICAL METHODS FOR MEASUREMENT OF ERYTHROCYTE CARBONIC ANHYDRASE --- p.22 / Chapter 4.1 --- DETERMINATION OF ECAI USING IMMUNOTURBIDIMETRIC ASSAY --- p.23 / Chapter 4.1.1 --- PRINCIPLE OF TECHNIQUE --- p.23 / Chapter 4.1.2 --- COLLECTION AND HANDLING OF BLOOD SPECIMENS --- p.29 / Chapter 4.1.3 --- PREPARATION OF HAEMOLYSATE --- p.31 / Chapter 4.1.4 --- MEASUREMENT OF HAEMOLYSATE CAI --- p.32 / Chapter 4.1.5 --- OPTIMIZATION PROCEDURE --- p.34 / Chapter 4.1.6 --- EVALUATION EXPERIMENTS --- p.36 / Chapter 4.1.7 --- DETERMINATION OF HAEMOGLOBIN CONCENTRATION IN HAEMOLYSATE --- p.39 / Chapter 4.1.8 --- DETERMINATION OF MEAN CELL HAEMOGLOBIN CONCENTRATION --- p.41 / Chapter 4.1.9 --- CALCULATION OF ERYTHROCYTE CARBONIC ANHYDRASE I CONCENTRATION --- p.43 / Chapter 4.2 --- DETERMINATION OF ECAI USING RADIAL IMMUNODIFFUSION METHOD --- p.43 / Chapter 5. --- RESULTS OF OPTIMIZATION AND EVALUATION EXPERIMENTS --- p.46 / Chapter 5.1 --- OPTIMIZATION RESULTS --- p.46 / Chapter 5.1.1 --- WAVELENGTH --- p.46 / Chapter 5.1.2 --- PEG CONCENTRATION --- p.49 / Chapter 5.1.3 --- SAMPLE VOLUME --- p.52 / Chapter 5.1.4 --- ANTIBODY DILUTION --- p.52 / Chapter 5.1.5 --- TEMPERATURE --- p.54 / Chapter 5.1.6 --- TIME OF INCUBATION --- p.54 / Chapter 5.1.7 --- OPTIMIZED TEST PROTOCOL --- p.56 / Chapter 5.2 --- EVALUATION RESULTS --- p.56 / Chapter 5.2.1 --- STABILITY --- p.56 / Chapter 5.2.2 --- LINEARITY --- p.58 / Chapter 5.2.3 --- PRECISION --- p.62 / Chapter 5.2.3.1 --- WITHIN-RUN --- p.62 / Chapter 5.2.3.2. --- BETWEEN-RUN --- p.62 / Chapter 5.2.4 --- CROSS-REACTIVITY --- p.62 / Chapter 5.2.5 --- RECOVERY --- p.62 / Chapter 5.2.6 --- COMPARISON WITH COMPARATIVE METHOD --- p.64 / Chapter 5.3 --- DISCUSSION --- p.64 / Chapter 6. --- ECAI IN NORMAL SUBJECTS --- p.67 / Chapter 6.1 --- SUBJECTS AND METHOD --- p.67 / Chapter 6.2 --- RESULTS --- p.67 / Chapter 6.3 --- DISCUSSION --- p.67 / Chapter 7. --- PATIENT STUDY --- p.69 / Chapter 7.1 --- SUBJECTS AND METHOD --- p.69 / Chapter 7.2 --- RESULTS --- p.70 / Chapter 7.2.1 --- DEMOGRAPHIC AND BIOCHEMICAL PARAMETERS --- p.70 / Chapter 7.2.2 --- CORRELATION BETWEEN ECAI AND FT3 RESULTS OF THYROTOXIC PATIENTS BEFORE TREATMENT --- p.71 / Chapter 7.2.3 --- CORRELATION BETWEEN ECAI AND EZN RESULTS OF THYROTOXIC PATIENTS --- p.71 / Chapter 7.2.4 --- RELATIONSHIP BETWEEN THE CHANGES IN ECAI AND FT3 RESULTS IN THYROTOXIC PATIENT ON TREATMENT --- p.71 / Chapter 7.3 --- DISCUSSION --- p.76 / Chapter 8. --- GENERAL DISCUSSION --- p.79 / Chapter 9. --- REFERENCES --- p.83