The cheetah (Acinonyx jubatus) has experienced a drastic decline in numbers over the last 20 years globally and is currently listed as vulnerable by the International Union for the Conservation of Nature (IUCN). In South Africa, there are only an estimated 763 free-ranging cheetahs and conflict with humans is arguably the most significant reason for this low number. The aim of my study was to determine the population size and demographic characteristics of the cheetah population within the Timbavati Private Nature Reserve (TPNR), South Africa, and to contribute to a better understanding of cheetah space use and habitat selection. The research was conducted on TPNR between November 2009 and June 2011 and I used a photographic survey to assess cheetah population size and demographic characteristics. Location data was obtained by collaring two adult male cheetahs with GPS/GSM collars and ad hoc sightings data from across the reserve for an adult female with cubs and three adolescent females. A relatively high minimum population density of 4.46 cheetahs/100km² was estimated, signifying a relatively healthy cheetah population. The sex ratio data indicated a higher male to female ratio and an average litter size of three cubs. The relatively high cub survival rate and density is promising in terms of the status of species within the area, as the data denote the success and potential persistence of the species. Cheetah home ranges varied between 20.97km² for the female with cubs and 659.65km² for the younger collared male. Season did not appear to be a determining factor in terms of home range sizes for the three social groups within the TPNR. However, the males did show a slight increase in their home range sizes during the dry season when resources where presumably more widespread. My results indicate that the cheetah is an adaptable species, flexible in behaviour and able to tolerate a variety of habitat types. Such knowledge is fundamental for planning and implementing the effective management and conservation of cheetahs in South Africa.