A time spectral and coupled adjoint based sensitivity analysis of rotor blade is carried out in this study. The time spectral method is an efficient technique to solve unsteady periodic problems by transforming unsteady equation of motion to a steady state one. Due to the availability of the governing equations in the steady form, the steady form of the adjoint equations can be applied for the sensitivity analysis of the coupled fluid-structure system. An expensive computational time and memory requirement for the unsteady adjoint sensitivity analysis is thus avoided. A coupled analysis of fluid, structural, and flight dynamics is carried out through a CFD/CSD/CA coupling procedure that combines FSI analysis with enforced trim condition. Coupled sensitivity analysis results and their validations are presented and compared with aerodynamics only sensitivity analysis results. The fluid-structure coupled adjoint based sensitivity analysis will be applied to the shape optimization of a rotor blade in the future work. Minimization of required power is the objective of the optimization problem with constraints on thrust and drag of the rotor. The bump functions are considered as the design variables. Rotor blade shape changes are obtained by using the bump function on the surface of the airfoil sections along the span. / Doctor of Philosophy / The work in this dissertation is motivated by the reducing the computational cost at the early design stage with guaranteed accuracy. In the research, the author proposes that the goal can be achieve through coupled adjoint based sensitivity analysis using a fluid structure interaction in time spectral form. Adjoint based sensitivity analysis is very efficient for solving design problems with a large number of design variables. The time spectral approach is used to overcome inefficient calculation of rotor flows by expressing flow and structural state variables as Fourier series with small number of harmonics. The accuracy and the efficiency of flow solver are examined by simulating UH-60A forward flight condition. A significant reduction in the computational cost is achieved by its Fourier series form of the periodic time response and the assumption of periodic steady state. A good agreement between time accurate and time spectral analysis is noted for the high speed forward flight condition of UH-60A configuration. Prediction from both methods also agree quite well with the experimental data. The adjoint based sensitivity analysis results are compared with the finite difference sensitivity analysis results. Even with presence of small discrepancies, these two results show a good agreement to each other. Coupled sensitivity analysis includes not only the effect of fluid state changes but also the contribution of structural deformation.