Thesis (M.S.)--West Virginia University, 2007. / Title from document title page. Document formatted into pages; contains vi, 53 p. : ill. Vita. Includes abstract. Includes bibliographical references (p. 50-51).

Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 123-151).

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Call number: LD2668 .T4 1979 G34 / Master of Science

Microvitellogenin is a female specific yolk protein from the tobacco hornworm moth Manduca sexta. A cDNA library was constructed from poly (A)⁺ RNA isolated from adult female fat body. cDNA clones of mRNA for microvitellogenin were isolated by screening the cDNA library with antiserum against microvitellogenin. The results of Northern blot analysis and hybrid selection indicated that the cDNA clone was specific for microvitellogenin. The complete nucleotide sequence of the 834 base pair cDNA insert has been determined by the dideoxy chain termination method. The deduced amino acid sequence was compared with the N-terminal sequence determined by Edman degradation, an amino terminal extension of 17 amino acids appeared to be a signal peptide. The cDNA sequence predicts that the mature microvitellogenin is a protein of 232 amino acids with a calculated molecular weight of 26,201. A comparison of the translated amino acid sequence with the sequences in National Biomedical Research Foundation protein library did not establish any sequence similarity with known proteins. The microvitellogenin gene begins to be expressed in the fat body on the first day of the wandering (prepupal) females as determined by using the cDNA insert as a probe to hybridize with the mRNA for microvitellogenin. The cDNA probe was also used to screen a genomic library of M. sexta, yielding three genomic clones for microvitellogenin. One of them was characterized and it contained the complete microvitellogenin gene. The gene sequence was determined. Comparison to the cDNA sequence showed that the microvitellogenin gene contains an intron near the 5'-end of the non-coding region. The 5'-flanking sequence of the gene has been compared to the same regions of yp genes of Drosophila and vitellogenin genes of locust, some similar sequences have been observed and discussed.

In vivo protein turnover was measured during the last 4 days of flight muscle development in tobacco hornworm pupa. Linear synthesis rates were measured up to 2 hours after injection of 30 μmol (3H) phenylalanine. Since the results with this technique did not differ from another established method, the large bolus injection of phenylalanine did not affect protein synthesis. The former method is advantageous because only a single time point is required. Flight muscle growth and protein synthesis decreased in parallel between 100 and 24 hours. During this time free phenylalanine turnover decreased, and the total pool diminished, indicating that this pool could be a major sink for muscle protein synthesis. Proteolysis was rapid even in the growing muscle. 20-Hydroxyecdysone increased muscle growth at certain times by inhibiting proteolysis. Protein synthesis either decreased or was unchanged after injection of the hormone. Therefore ecdysteroids may play a role in controlling growth of the dorsolongitudinal flight muscle during adult development, especially by retarding proteolysis.

Thesis (M.S.)--State University of New York at Binghamton, Department of Systems Science and Industrial Engineering, Thomas J. Watson School of Engineering and Applied Science, 2007. / Includes bibliographical references.

Thesis (Ph.D.)--Tufts University, 1998. / Adviser: Barry A. Trimmer. Submitted to the Dept. of Biology. Includes bibliographical references (leaves 92-105). Access restricted to members of the Tufts University community. Also available via the World Wide Web;

Eight-hundred and forty-eight tobacco (Protoparce sexta (Johan.)) and tomato (P. quinquemaculata (Haw.)) hornworm moths were irradiated individually with energy bands, nominally 33 Angstroms wide, centered at 3129, 3341, 3654, 4047, 4358, 4916, 5461, and 5780 A. Tests were made in a light-tight, air conditioned chamber. An analysis of variance was used to determine differences in responses to treatments. A significant difference in response to waveband treatments was found in each moth group. Generally, responses to the four shorter wavelengths were greater than to the four longer. When considering all moths or all trapped moths, best responses were to 3129 and 3341 Angstroms and second best were to 3654 and 4047 Angstroms. Reared moths were more responsive to treatments than trapped moths. Greatest response was obtained from the reared male tobacco hornworm moths. Tomato hornworm moths were less responsive to treatments than tobacco hornworm moths. The female tomato hornworm moths 1rJere numerically, least responsive. Numerically, more responses were elicited from first exposures than from those received later. / Master of Science