Thesis (Ph. D.)--University of Texas at Austin, 2008. / Vita. Includes bibliographical references.

Abdulrahman Al-Humiany (1999) A Comparison of the Responses to Enviromental Stress of the Gram-Positive Bacterium Staphylococcus xylosus and the Gram-Negative Bacterium Halomonas Halo. PhD Thesis, Department of Molecular Biology and Biotechnology, University of Sheffield. Salt tolerance of the Gram-negative bacterium, Halomonas Halo, was compared with the salt tolerance of a newly isolated Gram-positive coccus Staphylococcus xylosus. Both organisms grew over a range of salinities from 0.1 - 3.0 M NaCI in both rich medium containing yeast extract and in minimal medium. In the absence of yeast extract, growth of S. xylosus was very slow at 3.0 M NaCl and its optimum salinity for growth was 0.1 M NaCl, whereas Halomonas Halo showed optimum growth at 0.5 M NaCl. Growth experiments replacing NaCI with KC1 and the effect of Na+ on the rate of respiration showed that Halomonas Halo had a greater requirement for Na+ for growth than S. xylosus. When betaine was added to the minimal medium, it greatly increased the growth rate of both organisms at 3M NaCl. The precursor of betaine, choline, was also effective in increasing the growth rate of Halomonas Halo, but was much less effective for S. xylosus. Both organisms transported betaine into the cells by an energy dependent transport system; transport rates were broadly similar, but it appeared that the halotolerant S. xylosus took up betaine more efficiently than Halomonas Halo. Halomonas Halo and S. xylosus were shown to grow across a pH range from 5.5 - 8.5, but S. xylosus showed optimum growth across the full range whereas Halomonas Halo showed a distinct optimum at pH 7.0. The proton motive force (Ap) was found to be low in both organisms and at pH 8.5, it fell below the theoretical minimum (150 mV) which is required for ATP synthesis. Ap was significantly reduced by the inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and to a much lesser extent by monensin. Both inhibitors completely stopped the growth of both organisms at pH 7.0. The possibility that compatible solutes may protect enzymes from thermal denaturation was examined, but the results were inconclusive.

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