Thesis (M.S.)--California Polytechnic State University, 2010. / Mode of access: Internet. Title from PDF title page; viewed on April 6, 2010. Major professor: James Ahern. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Agribusiness." "March 2010." Includes bibliographical references (p. 63-66).

Thesis (M.S.)--West Virginia University, 2005. / Title from document title page. Document formatted into pages; contains vii, 79 p. : ill. (some col.), col. map. Includes abstract. Includes bibliographical references (p. 68-75).

Thesis (Ph.D.)--University of Delaware, 2006. / Principal faculty advisors: Thomas Evans, and Hugh Frick, Dept. of Plant and Soil Science. Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xv, 104 p.; also includes graphics (some col.). Includes bibliographical references (p. 93-104). Available online via OhioLINK's ETD Center

Dissertation (PhD)--University of Stellenbosch, 2007. / Bibliography. Also available via the Internet.

Thesis (Ph. D.)--Ohio State University, 2007. / Full text release at OhioLINK's ETD Center delayed at author's request

Made available in DSpace on 2014-10-09T12:51:11Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:56:13Z (GMT). No. of bitstreams: 1 11252.pdf: 6025397 bytes, checksum: 922a7a46ef469bfb01cf33facd40b567 (MD5) / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Orientador: Antonio Carlos Busoli / Banca: Arlindo Leal Boiça Junior / Banca: Marcos Doniseti Michelotto / Resumo: Spodoptera cosmioides Walker, 1858 é uma espécie polífaga, resistente a inseticidas em várias regiões do país e, apesar de ocorrer em baixas densidades, é considerada uma praga potencial para as culturas de algodão, soja e feijão no cerrado. Neste trabalho, estudou-se a biologia comparada de S. cosmioides sobre a cultivar transgênica comercial NuOPAL (Bollgard I, Evento 531) e sobre a cultivar convencional DeltaOPAL, procurando-se detectar a influência da α -endotoxina CryIAc no desenvolvimento biológico dessa espécie. O experimento foi desenvolvido em condições de laboratório (26 ± 1ºC; UR: 70 ± 10%; fotofase: 12h), a partir de lagartas recém-eclodidas e individualizadas. Foram avaliadas a duração e a viabilidade das fases imaturas, duração do ciclo biológico, peso de pupas, razão sexual, porcentagem de deformação de adultos e de adultos não liberados dos invólucros pupais, longevidade dos adultos, fecundidade e viabilidade de ovos. Os parâmetros biológicos observados não diferiram significativamente em relação às duas variedades. Na cultivar NuOPAL verificou-se maior porcentagem de ovos inviáveis, de adultos deformados e de adultos não emergidos, o que indica que essa cultivar provavelmente afeta a fase adulta de S. cosmioides. / Abstract: Spodoptera cosmioides Walker, 1858 is a polyphagous species, presents resistance to insecticides in various regions of Brazil, and although it occurs at low densities, it's considered a potencial pest in the crop of cotton, soybeans and beans in the Brazilian cerrado. In this work was studied the comparative biology of S. cosmioides on the comercial transgenic NuOPAL (Bollgard I, Evento 351) and the conventional cultivar DeltaOPAL to detect the influence of endotoxin-α CryIAc on the biological development of this species. The experiment was conducted under specific conditions in a laboratory (26 ± 1º C; RH: 70 ± 10%; photoperiod: 12h), from newlyhatched and individualized larvae. The duration and viability of immature phases and biological cycle, weight of pupae, sex ratio, percentage of deformed adults and adults not released the pupal beg, longevity of adults, fecundity and viability of eggs were evaluated. The biological parameters observed showed that there weren't significant difference between two varieties studied. In NuOPAL cultivar there was a greater percentage of eggs unviable, deformed adults and adults not emerged, which indicates that this cultivar probably affects the adult phase of S. cosmioides. / Mestre

Made available in DSpace on 2014-10-09T12:51:11Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:56:13Z (GMT). No. of bitstreams: 1 11252.pdf: 6025397 bytes, checksum: 922a7a46ef469bfb01cf33facd40b567 (MD5) / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

This project is focused on transformation of Silene vulgaris mediated by Agrobacterium tumefaciens and A. rhizogenes. S. vulgaris is a good model plant to study gynodioecy, an evolutionary step from bisexuality to dioecy. Gynodioecious plants form in some individuals bisexual flowers, while the others possess only female flowers. The aim of this research is do develop a technique to introduce foreign genes into this plant to study its developmental consequences. Using A. rhizogenes we successfuly prepared hairy root cultures, which unfortunately do not form shoot regenerants. We have prepared a protocol to induce plant regenerants from S. vulgaris leaf fragments. The first results do not confirm that A. tumefaciens infected plant regenerants harbor reporter transgenes. We used Nicotiana tabacum as a positive control.