Leaf area and stomata are important parameters in studies of taxonomic classification since both parameters might reflect the plant adaptation mechanisms to different environmental conditions. This study analyzed Eurycoma longifolia Jack leaf traits in different ecological regions (moisture zone: A Luoi, Bach Ma, Nam Dong; dry zone: Phong Dien) and leaves of seedlings in the nursery. The study used scanners and Images to measure leaf area; imagej and the AxioVision SE64 softwares were used to evaluate stomatal density by separating the lower cuticle through a clear nail polish impression. Our results showed that the leaf area of mature trees from the dry zone is smaller (355.7 and 484.1 cm2/leaf), however, the stomata density is higher than that of the humid area (284.4 and 137.9 stomata/mm2). Under the same nursery conditions, the seedlings' leaf area was similar while seedlings' stomatal density, originated from the dry zone, was lower. Results indicate that the stomatal density changes as an adaptation to changing habitat conditions. This study gives indicates that E. longifolia is a tree species with high adaptability given the leaf morphology changes under a changing climate. Therefore, this tree species should be a priority for planting in order to increase biodiversity in different ecological regions. / Đặc điểm diện tích lá và mật độ khí khổng là những thông số quan trọng trong các nghiên cứu phân loại học nhằm phản ánh cơ chế thích nghi của thực vật với điều kiện sống. Vì thế nghiên cứu này đã tiến hành phân tích các đặc điểm của lá cây Bách bệnh (Eurycoma longifolia Jack) từ các vùng khác nhau (A Lưới, Bạch Mã, Nam Đông: vùng ẩm và Phong Điền: vùng khô) và lá cây con của chúng ở vườn ươm. Nghiên cứu đã sử dụng máy quét và ImageJ để đo diện tích lá và phần mềm AxioVision SE64 và ImageJ để đánh giá mật độ khí khổng thông qua tách lớp biểu bì phía dưới lá qua lớp sơn mỏng Kết quả nghiên cứu cho thấy, diện tích lá của cây trưởng thành từ vùng khô nhỏ hơn (355.7 and 484.1 cm2/leaf) nhưng mật độ khí khổng lại lớn hơn so với vùng ẩm (284.4 and 137.9 stomata/mm2). Tuy nhiên, cùng điều kiện sống ở vườn ươm, diện tích lá của cây con tương tự nhau trong khi mật độ khí khổng của cây con có nguồn gốc từ vùng khô lại thấp hơn. Kết quả này chỉ ra rằng sự thay đổi của mật độ khí khổng như là một sự thích nghi với thay đổi của điều kiện môi trường sống. Từ đó nghiên cứu này cho thấy cây Bách bệnh là một loài cây gỗ với khả năng thích nghi cao nhờ sự thay đổi về đặc điểm hình thái lá khi thay đổi khí hậu cho nên loài cây này nên được ưu tiên trong việc gây trồng nhằm tăng cường tính đa dạng cho các vùng sinh thái khác nhau.

Made available in DSpace on 2015-03-26T13:53:35Z (GMT). No. of bitstreams: 1 resumo.pdf: 975706 bytes, checksum: b7f729aac6cddff22d4ac5ac02bd1e93 (MD5) Previous issue date: 2008-12-05 / The biome of the Atlantic Forest has sufered a continuous devastation process, with vast ecological and environmental consequences, and the main form of forest restoration is the planting of seedlings. The production of native tree species seedlings of the Atlantic, the hydroeletric plant of Itaipu Binational aims to reforest the riparian vegetation of ltaipu Lake, and constitute the Biological Corridor which will connect Iarge areas of protected forest in Brazil, Paraguay and Argentina. This venture is considered by UNESCO one of the largest environmental conservation projects of the world and includes the Morro do Diabo State Park, in the extreme west of São Paulo, the lguaçu National Park. between Paraná and Mato Grosso do Sul the green bands around the ltaipu power plant reservoir, the lguaçu and the lguazú National Park in Argentina, and the Turvo State Park in the state of Rio Grande do Sul. ln seedling production, the company gardens use a commercial substrate and plastic tubes of 115 cm3 of capacity. The experiments were conducted in the greenhouse of the Soil Department of the Federal University of Viçosa to evaluate the effects of macronutrients doses (N, P, K, Ca, Mg and S) on the growth of Cedrella fissilis Vellozo (Cedro), Peltophorum dubium (Spreng.) Taub (Canafístula) and Tabebuia serratifolia (Vahl) Nichols. (Ipê-amarelo), produced in tubes and substrate. The seedlings after 90 days of emergency received five doses of each macronutrient by cover application. The experimental design used was the completely randomized, with three species, five doses, six nutrients, with five repetitions with four plants each, towling 90 treatments and 1800 plants. After l40 days, for each species it was evaluated the number of leaves, stems, diameter and height of the neck, and determined the total dry weight and subdivided into leaves, stem, aerial part (MSPA), the dry mass relation of the aerial part with the root. We also evaluated the Ievels of N, P, K, Ca, Mg and S in leaves and roots. The increase of N doses províded significant growth for the three evaluated species. The increase of Mg doses significantly influenced the growth characteristics in canafístula plants and Ipê-amarelo. The increase of S doses favored the increments in plant height characteristícs of Ipê-amarelo. For the nutrients that provide significant responses by the regression analysis the recommended doses for maximum production were in the order of 200 mg dm-3 N, 240 mg dm-3 of Mg and 100 mg dm-3 of S. For the zero dose, the N in the maximum applied dose (200 mg dm-3) for plants of Cedro, Ipê-amarelo and Canafístula provided an increase of 6.4, 20.5 and 20.8% in MSPA. Rcspectively, the Mg in the maximum evaluated dose (240 mg dm-3) for seedlings of Ipê-amarelo and Canafístula provided an increment of 7.7 and 8.3% in MSPA, respectively, and S for the ipe scedlings. in the maximum evaluated dose (100 mg dm-3) provided an increase of 5.5% in the MSPA. The critical levels of N in the leaf tissues in Cedro plants, Canafístula and Ipê-amarelo, were 23.07, 20.61 and 22.68 g kg-1, and in the roots 10.83, 9.8 and 14. 19 g kg-1, respectively. The critical Mg levels in lhe leaf tissues in Canafístula and lpê-amareelo plants. were 0.96 and 1.76 g kg-1, and in the roots 0.57 and l.l9 g kg-1, respectively. The critical S levels in lpê-amarelo plants in the leaf tissues. were O.49 g kg-1 and 0.52 g kg-1 in the roots. The other nutrients did not show the recommended doses for the maximum production, justified probably by the presence of these nutrients in the substrate, near or above the critical level. ln general, the response of significant growth with the increase of N availabilíty is possibly Iinked to favorable conditions of leaching in the production of forest species grown in plastic tubes. For the advantage of the balance and availability of nutrients in the commercial substrate used, it is recommended the use of additional fertilization in Mg substrate for Canafístula and Ipê-amarelo, S for the lpê-amarelo and N for the three species. / O bioma da Mata Atlântica tem sofrido um processo contínuo de devastação, com grandes consequências ecológicas e ambientais, e a principal forma de recomposição florestal é o plantio de mudas. A produção de mudas de espécies arbóreas nativas da Mata Atlântica, na Usina Hidrelétrica de Itaipu Binacional tem o objetivo de reflorestar a mata ciliar do lago de Itaipu, e constituir o Corredor Biológico que interligará grandes áreas de mata protegida no Brasil, Paraguai e Argentina. Este empreendimento é considerado pela UNESCO um dos maiores projetos de conservação ambiental do mundo e inclui o Parque Estadual do Morro do Diabo, no extremo oeste de São Paulo; o Parque Nacional de Iguaçu, entre Paraná e Mato Grosso do Sul; as faixas verdes em torno do reservatório da usina de Itaipu; o Parque Nacional do Iguaçu e o de Iguazú, na Argentina; e o Parque Estadual do Turvo, no Rio Grande do Sul. Na produção de mudas, os hortos da empresa utilizam substrato comercial e tubete de 115 cm3 de capacidade. Os experimentos conduzidos em casa de vegetação do Departamento de Solos da Universidade Federal de Viçosa objetivaram avaliar os efeitos de doses dos macronutrientes (N, P, K, Ca, Mg e S). sobre o crescimento de Cedrella fissilis Vellozo (Cedro), Peltophorum dubium (Spreng.) Taub (Canafístula) e Tabebuia serratifolia (Vahl) Níchols. (lpê-amarelo), produzidas em tubete e substrato. As plântulas após aproximadamente 90 dias de emergência receberam cinco doses de cada macronutriente via aplicação em cobertura. O delineamento experimental utilizado foi o inteiramente casualizado, sendo três espécies, cinco doses, seis nutrientes, com cinco repetições com quatro plantas cada, totalizando 90 tratamentos e 1800 plantas. Após 140 dias, foi avaliado para cada espécie o número de folhas, hastes, diâmetro do colo e altura, e determinado a matéria seca total e subdividida em folha, caule, parte aérea (MSPA), de raízes, a relação da massa seca da parte aérea com a raiz. Foram avaliados ainda os teores de N, P, K. Ca. Mg e S nas folhas e raízes. O aumento das doses de N proporcionou o crescimento significativo para as três espécies avaliadas. O aumento das doses de Mg influenciou as características de crescimento significativamente nas plantas de canafístula e Ipê-amarelo. O aumento das doses de S favoreceu os incrementos nas características de crescimento das plantas de Ipê-amarelo, para a produção máxima foram da ordem de 200 mg dm-3 de N, 240 mg dm-3 de Mg e 100 mg de de S. Em relação a dose zero, o N na máxima dose aplicada (200 mg dm-3), para as plantas de Cedro, Canafístula e Ipê-amarelo, proporcionou um incremento de 6,4, 20,5 e 20,8 % na MSPAA respectivamente; 0 Mg na dose máxima avaliada (24O mg dm-3), para as mudas de Canafístula e Ipê-amarelo proporcionou um incremento de 7,7 e 8,3 % na MSPA, respectivamente e o S para as mudas de Ipê-amarelo, na dose máxima avaliada (100 mg dm-3 ), proporcionou um incremento de 5.5 % na MSPA. Os teores críticos de N nos tecidos foliares nas plantas de Cedro, Canafístula e Ipé-amarelo, foram de 23,07, 20,61 e 22,68 g kg-1, e nas raízes 10,83, 9,8 e 14,19 g kg-1, respectivamente. Os teores críticos de Mg nos tecidos foliares nas plantas de Canafístula e Ipê-amarelo, foram de 0.96 e I,76 g kg-1, e nas raízes 0,57 e l,l9 g kg-1, respectivamente. Os teores críticos de S nas plantas de Ipê-amarelo, nos tecidos foliares, foram de 0,49 g kg-1 e 0.52 g kg-1 nas raízes. Os demais nutrientes não apresentaram as doses recomendadas para a produção máxima, justificado, provavelmente pela presença destes nutrientes no substrato, próximo ou acima do nível crítico. Em geral a resposta de crescimento significativo com o aumento da disponibilidade de N, possivelmente está ligada as condições favoráveis de lixiviação na produção de espécies florestais produzidas cm tubetes. Para o favorecimento do equilíbrio e disponibilidade de nutrientes no substrato comercial utilizado, é recomendado o uso de adubação complementar em substrato de Mg, para a Canafístula e Ipê-amarelo, de S para o Ipê-amarelo e de N para as três espécies.

Subsistence harvesting pressure in most African countries focuses on the small and mostly unreproductive trees found in the understorey stratum and can have potentially insidious ecological effects. Harvest intensities at Ongoye Forest Reserve (OFR) vary significantly across the forest (range = 87 - 567 stumps ha-1), with harvesting focussed exclusively on poles from tree species that grow only in the understorey. Growing evidence indicates that seedling establishment from the pool of species available beneath a closed canopy is greatly influenced by the differential ability of species to take advantage of the short burst of resources in newly-created understorey gaps. Seedling dynamics in these gaps may determine forest tree diversity and dynamics and consequently harvest gaps have the potential to significantly affect natural forest dynamics. This study examined seedling establishment beneath intact understorey and within artificially created understorey gaps of different sizes (single stem gaps, two stem gaps, four stem gaps, eight stem gaps and control ‘gap’, where no stems were removed) that simulated different spatial scales of harvesting intensity of understorey trees. This experiment examined the proposition that successful seedling establishment and natural succession is strongly dependent on the scale of harvesting. Seedling abundance, species richness, irradiance (photosynthetically active radiation and the red to far-red ratio), soil nutrient composition and herbaceous layer cover was measured in each gap size in 2005 before harvesting, and again in 2007. The mean seedling abundance was not significantly different among gap treatments, although there was a trend towards more seedlings in 2005 than 2007. Seedling abundance in all gaps was greater than at control sites beneath the intact understorey. There was a continuous increase in seedling richness in 2007. An average species richness of 4.3 was recorded in the control sites, beneath a shaded understorey. Here, seedling richness increased by 18.24% with the removal of a single understorey tree (Gap 1). Species richness increased with increasing experimental gap size increased so that the greatest mean species richness (6.2 species) was recorded where eight neighbouring trees were removed (Gap 8). Light transmission reaching the seedling stratum was greater in larger gaps and there was a trend towards more seedlings and greater species richness in the higher light environments of such gaps. Soil nutrient levels did not influence seedling abundance and species richness in gaps. The herbaceous layer suppressed seedling establishment. In the largest gaps (115.4m2) created by harvesting, seedling composition was more deterministic than in small gaps where seedling establishment and density was random, accordingly there were more species in larger gaps from a more defined species assemblage. Current harvesting levels of pole-sized understorey trees, where only small gaps are created in the understorey, are unlikely to alter forest dynamics and species composition at OFR. This study demonstrates that harvesting eight adjacent trees crosses the harvest intensity threshold between sustainable natural tree dynamics and a potential successional shift to an alternative state. Clearly, for natural dynamics to be maintained harvesting intensities will have to be regulated. / http://hdl.handle.net/10413/631 / Thesis (M.Env.Dev.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.