The aim of this study is to examine the Christian community called Plymouth Brethren and how they interpret religion in relation to their entrepreneurship and business practices. The community has a strong faith in God, uses the Bible as the guiding principle in everyday life, and also has the ambition to live together within the group in seclusion from the rest of society. Based on interviews with Plymouth Brethren in the county of Småland, the study describes how they perceive the relationship between the Christian faith and entrepreneurship and practice religion in the context of their family business. As the study illustrates the Christian faith is significantly motivating their enterprises of business and the Bible is their guiding principle even when it comes to how they should run their businesses.