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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Effect of dietary fatty acids on the expression of the Fgf-3 gene and mouse mammary tumor virus in strain A/St mammary tumors

Halstead, Bartley W. January 1997 (has links)
The specific objective of this study was to determine if Fgf-3 gene expression is mediated by dietary fatty acids and to confirm mouse mammary tumor virus infection. It is well known that dietary linoleic acid enhances growth and dietary stearic acid inhibits growth of mammary tumors. Tumor RNA was extracted from female strain A/St mice fed one of four diets. A radioactively labeled anti-sense RNA probe was generated, invitro, from isolated and purified pFgf-3c (int-2c clone contained in the vector pSP65). The Fgf-3c probe was hybridized to extracted tumor RNA using the ribonuclease protection assay.Electron microscopy confirmed MMTV infection by visualization of type A and B particles in tumor tissue. Expression of Fgf-3c, qualified by RNase protection assay, ranged from 0.02 to 5.89 (relative band density) in all of the diet groups. A positive association between Fgf-3c expression and weight was observed among the tumors of the SA-1 diet (R = 0.947). The SF, SF-1, and PA experimental diets, individually, did not appear to show strong correlation with respect to tumor size. Fgf-3 expression was less in small tumors (<275 mg) and enhanced in large tumors (>275 mg) (p<0.05). / Department of Biology

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