Lichen and moss are the dominant ground cover on the Canadian Shield rock barrens of eastern Georgian Bay, and they provide many ecosystem services. Lichen and moss mats are essential for developing and accumulating soil on the bedrock landscape, and as the mats establish they moderate soil temperature and reduce soil water losses, thereby improving the microclimate for more complex vegetation. In addition to pioneering ecosystem succession, the lichen and moss mats provide essential nesting habitat for turtle species-at-risk. These lichen and moss mats are not well understood on rock barrens landscapes, and as such this thesis aims to increase knowledge of the growth, persistence and restoration approaches for these valuable ecosystem resources. We quantified the ecohydrological controls on the growth of lichen and moss mats by measuring the CO2 exchange of lichen and moss under varying environmental conditions. From these results we determined that key growth periods for lichen and moss were during the wet portions of the growing season (spring and fall), and that growth was limited or non-existent during the dry period (summer). Further, we determined that soil moisture was the most important control on lichen and moss CO2 exchange, and that this relationship differed among cover type (lichen, moss, mix of lichen and moss). Moss was able to continue CO2 uptake at a lower water content than lichen, suggesting that lichen would have a greater decline in productivity under drier conditions. A decline in lichen and moss productivity would also likely lead to a decline in soil development through chemical weathering which, in turn, could affect the availability of turtle nesting habitat. We also used CO2 exchange measurements to compare lichen and moss productivity between natural and transplanted mats. Transplanting in-tact patches of lichen and moss has not been widely studied, and as such we tested this approach on a rock barrens landscape. We determined that natural and transplant productivity did not differ for lichen, and that there were some differences between treatments for mixed and moss plots. We also used the tea bag index method to compare relative decomposition rates between treatments (natural, transplant), where we found that decomposition rates did not differ. Our results indicate that it is feasible to remove lichen mats from the footprint of a planned disturbance such as construction and transplant them successfully to nearby undisturbed areas. This approach would restore the lichen cover and the ecosystem services that lichens provide immediately rather than waiting decades for natural regrowth or fragment establishment. As a whole, this thesis will increase knowledge of both the growth and persistence, as well as the restoration of lichen and moss on rock barrens landscapes. Given that lichens and mosses of these genera grow globally, our findings can be applied widely to enhance and protect lichen and moss mats, and the ecosystem services they provide / Thesis / Master of Science (MSc)

Climate change poses a serious threat to species that demonstrate temperature dependent sex determination (TDS), including marine turtles. Increased temperatures can result in highly female skewed sex ratios and decreased hatchling success. In situ sand temperature data was collected from the nesting depth of hawksbill and green turtles at five study sites along the coast of the Red Sea. The sand temperature profile at four of the sites exceeded the pivotal temperature of 29.2°C (commonly cited in literature) throughout the study duration, which suggests feminization of turtles could be occurring, but further studies need to identify the pivotal temperature in this region. The percentage of days exceeding the commonly cited maximum thermal threshold (33 and 35°C) was calculated for each site at 30 and 50 cm. Sand temperature recordings were as high as 36.0°C at 30 cm depth, and 35.3°C at 50 cm. This suggests that the turtle hatchlings in some areas of the Red Sea could already have high mortality rates due to high temperatures, unless they are locally adapted to these high temperatures. The Red Sea is home to five out of the seven extant species of marine turtles in the world, but not much is known about these populations. The Red Sea is an understudied region of the world, but it has the potential to provide insight on how species might adapt to future climate change due to its high and variable water temperatures (range of 20°C to 35°C) and high salinity (40 PSU). Sites with lower sand temperatures (and lower risk of feminization) may represent priority areas for conservation efforts, particularly in regions facing imminent coastal development.

Reptiles are the vertebrate taxon with the highest percentage of at-risk species in Canada, many of which exist at the northern limit of their species’ home range in Ontario. Numerous reptiles are found in the Georgian Bay area; however, factors limiting their distribution in Ontario are poorly understood. It is likely that the thermal and hydrological conditions of a reptile’s critical habitat are contributing factors. Specifically, peatlands serve as ideal hibernacula for the threatened Eastern Massasauga Rattlesnake (Sistrurus catenatus) while moss cushions may provide freshwater turtles, including the threatened Blanding’s Turtle (Emydoidea blandingii) and endangered Spotted Turtle (Clemmys guttata), with nesting opportunities on open rock barrens. Although different in their functional purpose at opposite life stages for two separate orders of reptiles, these ecosystems provide suitable conditions to meet the physiological needs of the reptiles utilizing them. This analysis characterizes the thermal and hydrological conditions of moss-dominated ecosystems from a reptile species-at-risk perspective. The interaction between the water table and the frost line is important when assessing the winter survival of Eastern Massasauga Rattlesnakes hibernating in peatland hummocks. Larger hummocks are more ideal as they have a lower chance of becoming flooded in the winter while still providing protection from the advancing frost line. Ideally, hummocks that are 30-35 cm tall provide the greatest chance of survival when snakes hibernate 20-25 cm below the surface. Subsurface temperatures in relation to snow depth, as it is influenced by tree stand characteristics, was also assessed. This resulted in the conclusion that the presence, absence, timing, and frequency of freeze and thaw events is likely more important than snow depth when it comes to winter survival. Similar to rattlesnake hibernacula, turtle nests can be inundated for extended periods of time or exposed to extreme temperatures which reduces their chance of survival. The water storage dynamics of 22 hillslope and 12 hilltop moss cushions along with the temperature dynamics at nine locations were characterized. Although it was determined hilltop locations had greater water storage capabilities, continuously monitored moss cushions responded very quickly to rainfall events whereby sites were inundated for less than 12 hours. Average subsurface temperatures decreased with depth as did temperature fluctuations, both of which were positively correlated but not significantly affected by canopy openness. However, due to their tendency to grow on flat surfaces, not all moss cushions are suitable nesting sites. Moreover, temperatures that ensure proper development and equal ratios of male-to-female turtles were not achieved suggesting that this is a potential factor limiting the northern distribution of turtles in Ontario. In order to properly asses the vulnerability of these populations to threats including habitat loss and climate change, the habitat requirements of different species at critical life stages needs to be understood. Therefore, conservationists can use this study to implement mitigation strategies that consider impacts on the thermal and hydrological dynamics within reptile habitat. / Thesis / Master of Science (MSc)

I studied aspects of the ecology of the Murray River turtle, Emydura macquarii, to determine the impact of the introduced red fox, Vulpes vulpes. The fox is one of Australia's worst vertebrate pests through its predation on livestock and native mammals, but their impact on reptilian communities is not known. I conducted a large-scale mark-recapture study to evaluate population growth of E. macquarii in the Albury region of the upper Murray River by determining growth, reproduction and survival. The study was conducted downstream of the first, and largest, impoundment on the Murray River, Lake Hume. Emydura macquarii predominantly inhabit the lagoons in the upper Murray River, as the mainstream and Lake are possibly too cool to maintain metabolic processes. They are easily captured in hoop traps and the use of live decoys maximises trap success. Over 2000 hatchling turtles were marked and released into two lagoons between January 1997 and January 1998. Growth of these individuals is rapid over the first few years but declines towards maturity, and is indeterminate after maturity. Although growth annuli are not well defined, even on young individuals, the von Bertalanffy model describes the growth of both male and female E. macquarii. Male turtles mature at 5-6 years and females mature at 10-12 years. Female turtles may maximise reproductive potential by delaying maturity and producing one relatively large clutch (mean = 21 eggs) per year, which is positively correlated with body size (PL). Although primarily related to body size, clutch size varies annually because of environmental conditions. If winter and summer rainfalls are below average and temperatures are above average, E. macquarii may reduce clutch size to increase the chance of the eggs surviving. Nesting predominantly occurs during the first major rain-bearing depression in November. Habitat variables, including distance from water, nearest nest, and tree, and soil type were measured for each nest to determine characteristics that attract predators. Nests close to the shoreline and trees are heavily preyed on, and nests constructed in sand are less likely to be destroyed by predators. Foxes detect nests through a combination of chemical cues from eggs and slight soil disturbances, whereas birds only destroy nests observed being constructed during the day. Female turtles alter nesting behaviour and construct nests much further away from water when foxes were removed and as a result, nests are less dense and away from trees. Thus in high predation risk areas, turtles minimise emergence and search times to reduce the risk of direct predation by foxes. Predation is reduced when nests are in lower densities and away from trees, because predators increase search efforts when nests are in higher densities and birds are more likely to destroy nests close to trees. Reproductive success is further reduced in high predation risk areas because more nests are constructed in sandy substrates where clutch success is reduced compared to incubation in more dense substrates. Where predators are a significant source of mortality, prey may use indirect methods, such as chemical recognition, to avoid encounters. Nesting turtles did not avoid areas where fox odour was present, suggesting that they assess predation pressure from foxes by other mechanisms, such as visual recognition. However, an innate response occurs to the odour of a once common predator on the Murray River, the eastern quoll (Dasyurus viverrinus), whereby turtles recognise and avoid nesting in areas where quoll odour is present. Therefore nesting turtles show a similar avoidance response to two different predators, using different mechanisms of detection. Similarly, predation risk may influence hatching times and nest emergence. The rate of embryonic development of E. macquarii may increase or eggs may hatch early so that the clutch hatches synchronously, thereby reducing the risk of predation through group emergence from the nest. Emydura macquarii reach densities of over 100 turtles.ha-1, with the majority of the population consisting of sexually mature individuals. Emydura macquarii has a Type III survival curve where mortality is extremely high in the egg stage (93% nest predation), remaining high over the hatchling stage (minimum survival rate- 10%), but decreasing rapidly throughout the juvenile stage (~70% juvenile survival). Adult survival is extremely high, with greater than 95% of adults surviving each year. Foxes through nest predation cause most mortality but a small proportion (~3%) of nesting adult females are killed by foxes each year. A removal program evaluated the impact of foxes. In 1996, fox numbers were monitored around four lagoons by spotlighting and non-toxic bait uptake. Foxes were removed from around two of the lagoons throughout 1997 and 1998, using spotlight shooting and 1080 bait poisoning. Fox numbers were continually monitored around all four lagoons during the study. Nest predation rates remained around 90% in all sites where foxes were present, but fell to less than 50% when foxes were removed. At the same time, predation on nesting female turtles was eliminated where foxes were removed. Demographic models using staged based survival schedules, together with growth and fecundity values for E. macquarii show a decline of 4% per year in these populations. Elasticity analyses shows that survival of adult female E. macquarii has the major influence on population stability and a reduction of nest predation alone is unlikely to address the population decline. Management options, such as reducing foxes prior to nesting around key lagoons, will stabilise the population decline, and eliminating foxes completely from certain areas with high dispersal potential, will promote recruitment of juvenile E. macquarii.

I studied aspects of the ecology of the Murray River turtle, Emydura macquarii, to determine the impact of the introduced red fox, Vulpes vulpes. The fox is one of Australia's worst vertebrate pests through its predation on livestock and native mammals, but their impact on reptilian communities is not known. I conducted a large-scale mark-recapture study to evaluate population growth of E. macquarii in the Albury region of the upper Murray River by determining growth, reproduction and survival. The study was conducted downstream of the first, and largest, impoundment on the Murray River, Lake Hume. Emydura macquarii predominantly inhabit the lagoons in the upper Murray River, as the mainstream and Lake are possibly too cool to maintain metabolic processes. They are easily captured in hoop traps and the use of live decoys maximises trap success. Over 2000 hatchling turtles were marked and released into two lagoons between January 1997 and January 1998. Growth of these individuals is rapid over the first few years but declines towards maturity, and is indeterminate after maturity. Although growth annuli are not well defined, even on young individuals, the von Bertalanffy model describes the growth of both male and female E. macquarii. Male turtles mature at 5-6 years and females mature at 10-12 years. Female turtles may maximise reproductive potential by delaying maturity and producing one relatively large clutch (mean = 21 eggs) per year, which is positively correlated with body size (PL). Although primarily related to body size, clutch size varies annually because of environmental conditions. If winter and summer rainfalls are below average and temperatures are above average, E. macquarii may reduce clutch size to increase the chance of the eggs surviving. Nesting predominantly occurs during the first major rain-bearing depression in November. Habitat variables, including distance from water, nearest nest, and tree, and soil type were measured for each nest to determine characteristics that attract predators. Nests close to the shoreline and trees are heavily preyed on, and nests constructed in sand are less likely to be destroyed by predators. Foxes detect nests through a combination of chemical cues from eggs and slight soil disturbances, whereas birds only destroy nests observed being constructed during the day. Female turtles alter nesting behaviour and construct nests much further away from water when foxes were removed and as a result, nests are less dense and away from trees. Thus in high predation risk areas, turtles minimise emergence and search times to reduce the risk of direct predation by foxes. Predation is reduced when nests are in lower densities and away from trees, because predators increase search efforts when nests are in higher densities and birds are more likely to destroy nests close to trees. Reproductive success is further reduced in high predation risk areas because more nests are constructed in sandy substrates where clutch success is reduced compared to incubation in more dense substrates. Where predators are a significant source of mortality, prey may use indirect methods, such as chemical recognition, to avoid encounters. Nesting turtles did not avoid areas where fox odour was present, suggesting that they assess predation pressure from foxes by other mechanisms, such as visual recognition. However, an innate response occurs to the odour of a once common predator on the Murray River, the eastern quoll (Dasyurus viverrinus), whereby turtles recognise and avoid nesting in areas where quoll odour is present. Therefore nesting turtles show a similar avoidance response to two different predators, using different mechanisms of detection. Similarly, predation risk may influence hatching times and nest emergence. The rate of embryonic development of E. macquarii may increase or eggs may hatch early so that the clutch hatches synchronously, thereby reducing the risk of predation through group emergence from the nest. Emydura macquarii reach densities of over 100 turtles.ha-1, with the majority of the population consisting of sexually mature individuals. Emydura macquarii has a Type III survival curve where mortality is extremely high in the egg stage (93% nest predation), remaining high over the hatchling stage (minimum survival rate- 10%), but decreasing rapidly throughout the juvenile stage (~70% juvenile survival). Adult survival is extremely high, with greater than 95% of adults surviving each year. Foxes through nest predation cause most mortality but a small proportion (~3%) of nesting adult females are killed by foxes each year. A removal program evaluated the impact of foxes. In 1996, fox numbers were monitored around four lagoons by spotlighting and non-toxic bait uptake. Foxes were removed from around two of the lagoons throughout 1997 and 1998, using spotlight shooting and 1080 bait poisoning. Fox numbers were continually monitored around all four lagoons during the study. Nest predation rates remained around 90% in all sites where foxes were present, but fell to less than 50% when foxes were removed. At the same time, predation on nesting female turtles was eliminated where foxes were removed. Demographic models using staged based survival schedules, together with growth and fecundity values for E. macquarii show a decline of 4% per year in these populations. Elasticity analyses shows that survival of adult female E. macquarii has the major influence on population stability and a reduction of nest predation alone is unlikely to address the population decline. Management options, such as reducing foxes prior to nesting around key lagoons, will stabilise the population decline, and eliminating foxes completely from certain areas with high dispersal potential, will promote recruitment of juvenile E. macquarii.

The beaches of Broward County, Florida are a prevalent nesting site for loggerhead (Caretta caretta) sea turtles, however extensive beach erosion is threatening critical nesting habitat. Beach renourishment, the process of transporting offshore or upland sediment onshore, is a widely used method of replenishing lost sand. However, renourishment can negatively affect sea turtle nesting habitat by increasing beach compaction; the resistance to applied pressure in pounds per square inch (psi). Increased sand compaction impedes the digging of the female which affects nesting success. The influence of beach compaction on sea turtle nesting patterns has never been previously examined over the course of a nesting season on Hillsboro and Deerfield Beach. Therefore, this study was designed to examine beach compaction data for Hillsboro, a mostly natural beach, and Deerfield, a completely renourished beach, during the 2010 nesting season and analyze the compaction data against 2010 nest and false crawl (FC, non-nesting emergence) data. Compaction readings were collected during every other week March-October using a soil compaction meter at every other street address along three beach positions, the dune base, mid-beach, and average high tide line (HTL); and at three depths, 15 cm, 30 cm, and 45 cm. Values were not statistically different throughout the season for each beach, so seasonal mean compaction values were used for each beach position and depth. Hillsboro compaction values were rarely over 500 psi (35 kg/cm2), even at 45 cm depth. Deerfield compaction values exceeded the 600 psi (42kg/cm2) measurement limit of the meter in approximately 60% of the compaction values at 30 cm or 45 cm depth. Sand compaction data was analyzed for any trends between beaches as well as within each beach. Historical data shows higher loggerhead nesting success, the number of nests/total number of crawls (including FC) x 100, on Hillsboro Beach than on Deerfield Beach. The average beach compaction values were compared to nesting success and to nest and FC density within each station area. There was a significant inverse relationship (p<0.05) between beach compaction and nesting success at each of the beach positions and depths, when both Hillsboro and Deerfield Beaches were analyzed together, except at the Mid 30 cm and Dune 45 cm depth. The strongest relationship for the combined beaches was at the HTL 15 cm depth (R2=0.3821, p<0.001). When Hillsboro was analyzed alone, beach compaction and nesting success was only significantly inversely related (R2=0.0875, p<0.02) at the HTL 15 cm depth. This demonstrates that while increased beach compaction may partially influence nesting success, there are likely other beach characteristics that contribute to nest site selection of loggerheads in Northern Broward County. The inverse relationship between Hillsboro mean beach compaction and nest density (nests per meter) was significant only at the HTL 15cm depth (p<0.002) and the inverse relationship between mean beach compaction and false crawl density (FC per meter) in Hillsboro was only significant at the Dune 15 cm (p<0.019) and the Dune 30 cm (p<0.038) depths. Although, increased beach compaction was expected to relate to higher FC density, FC density showed a significant inverse relationship to mean beach compaction at all Deerfield Beach positions and depths in and this suggests off-shore factors may be affecting nest site selection.