Rotational grazing is being promoted by some land managers, government agencies, and conservation groups as superior to season-long grazing for improving pasture and cattle production, and for grassland bird conservation; however, the assumed benefits have not been comprehensively studied. In 2011 and 2012, I examined the effects of twice-over rotational grazing on the nesting success of grassland songbirds in southwestern Manitoba, Canada. I monitored nesting attempts and collected structural vegetation data for five species of obligate grassland bird (n=110) and one shrub-nesting species (n=41). Nesting analyses were conducted using logistic exposure models. Twice-over grazing had a significantly negative effect on the nesting success of the ground nesting species, including Savannah sparrows (Passerculus sandwichensis). Grazing system did not have an effect on vegetation structure. The results are consistent with other rotational grazing studies, and suggest that twice-over rotational grazing does not benefit grassland songbirds in mixed-grass prairie habitats. / October 2016