Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2018-01-26T17:43:24Z No. of bitstreams: 1 RAFAEL_DOS_SANTOS_MAZZUCA_DIS.pdf: 2501773 bytes, checksum: 8e77692cbd2f35eb9907d89c485c3237 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-02-05T11:21:14Z (GMT) No. of bitstreams: 1 RAFAEL_DOS_SANTOS_MAZZUCA_DIS.pdf: 2501773 bytes, checksum: 8e77692cbd2f35eb9907d89c485c3237 (MD5) / Made available in DSpace on 2018-02-05T11:44:18Z (GMT). No. of bitstreams: 1 RAFAEL_DOS_SANTOS_MAZZUCA_DIS.pdf: 2501773 bytes, checksum: 8e77692cbd2f35eb9907d89c485c3237 (MD5) Previous issue date: 2017-03-10 / Everyyear the number of people with diabetes increases considerably. By the year 2014, more than 414 million cases of the disease have been registered worldwide. A major problem is the increasing number of diabetic patients who develop ulcers in the lower limbs, a complication mainly due to inadequate glycemic control, besides the imbalance in the inflammatory phase during the process of tissue regeneration, which often leads to amputation of the lower limbs.The increase number ofcases andcomplications derived from the diabetesrequire the development of new therapeutic strategies to minimize the development of these ulcers to more severe cases new therapeutic strategies are necessary, aiming to minimize the development of these ulcers thatleads to a poor quality of life due to ulcers, and ultimately, amputations. Cell therapy associated with the use of biomaterials, produced through tissue engineering processes, represents a new alternative for current treatments. The use of bone marrow mononuclear cells has shown to be an important ally for the healing process, since they are a good quality and abundant cells of easy access. The purpose of this study was to evaluate whether the use of a biomaterial as a biological dressing, associated or not with bone marrow mononuclear cells, would improve the regenerative response of ulcer lesions, reducing the time of healing.Streptozotocin was used to induce diabetes in Wistar rats. To simulate an ulcer wound, a punch was created on the animal's back.Themacroscopic analysishave shown that the animals treated with the biomaterial associated with the bone marrow mononuclear cells, had a significantly better regeneration rate in the first 2 days after the formation the wound formation, when compared with the other groupswhere only the biomaterial was applied or did not received any treatment.In addition, histological sections stained with hematoxylin and eosin, allowed to define the degree of regeneration that each animalhave undergone, taking into account the reepithelialization and abundance of inflammatory infiltrate.Our results indicate that the use of the bone marrow mononuclear cellsfraction associated with the biomaterial may be a therapeutic ally in the regeneration of diabetes ulcers. / A cada ano o n?mero de pessoas com diabetes aumenta consideravelmente. At? o ano de 2014, foram registradosmais de 414 milh?es de casos da doen?aem todo o mundo. Um grande problema ? o crescente aumento non?mero de casos de pacientes diab?ticos que desenvolvem ?lceras nos membros inferiores, complica??o decorrente principalmentedo controle glic?micoinadequado, al?m do desequil?briona fase inflamat?ria durante o processo de regenera??o tecidual, que muitas vezes,acabalevando ? amputa??o dos membros inferiores. A partir deste panorama, doaumento de casos da doen?a e tamb?mdoaumento no n?mero de complica??es derivadas da diabetes, s?o necess?rias novas estrat?gias terap?uticas, que visem minimizar o desenvolvimento destas ?lceras at? casos mais graves.A terapia celular associada ao uso de biomateriais, produzidos atrav?s de processos deengenharia tecidual, representam uma nova alternativa para tratamentos atuais. O uso da fra??ode c?lulasmononuclearesda medula ?ssea temse mostrado um importante aliado neste processo, por ser uma fonte de f?cil acesso e com a possibilidade de obten??o de grande quantidade de c?lulas. Este estudoteve como objetivo principal avaliar se o uso de um biomaterial como curativo biol?gico, associado ou n?o ?s c?lulas da fra??o mononuclear da medula ?ssea, seria capaz de melhorara resposta regenerativa de les?es ulcerosas,reduzindoo tempo de cicatriza??o.Por meio do uso da estreptozotocina,foi poss?vel criar um modelo animal de diabetesem ratos Wistar, seguido da realiza??o deuma les?o no dorso do animal para simular uma ferida ulcerosa, frequentemente presente em membros inferiores de diab?ticos.Por meio de an?lises macrosc?picas, com o uso de imagens, foi poss?vel observar que os animais tratados com o biomaterial associado?fra??o de c?lulas mononuclearesda medula ?ssea, tiveram uma taxa de regenera??osignificativamente melhor nos primeiros 2 dias ap?s a forma??o da les?o, quando comparado com os outros gruposaos quais foram aplicados somente o biomaterial ou sem tratamento.Al?m disso, an?lises dos cortes histol?gicos corados comhematoxilinaeeosina, possibilitaramdefiniro grau de regenera??o de cada animal, levando em considera??o a reepiteliza??o da les?o e a abund?ncia de infiltrado inflamat?rio.Os resultados obtidosindicam que o uso da fra??o de c?lulas mononucleares da medula ?ssea associado ao biomaterial pode ser um aliado terap?utico na regenera??o de ?lceras de diabetes.