Thesis (Ph. D.)--Institute of Paper Chemistry, 1942. / Includes bibliographical references (p. 68).

Thesis (Ph. D.)--Institute of Paper Chemistry, 1940. / Includes bibliographical references (leaves 44-45).

Thesis (Ph. D.)--Institute of Paper Chemistry, 1941. / Bibliography: leaves 91-93.

Thesis (M.S.)--University of North Carolina at Wilmington, 2003. / Includes bibliographical references (leaves : [51]-54).

Photodissociation methods have been explored for structural analysis of peptides and oligosaccharides. Ultraviolet photodissociation (UVPD) was applied to carboxylated derivatized peptides and reducing end derivatized oligosaccharides which offer selective dissociation and specific fragmentation pathways in comparison to CID. Upon UVPD of the modified peptides at carboxylate comprised of reduced y ions and increased immonium ions. The derivatized oligosaccharides via reductive amination and hydrazide conjugation can undergo highly efficient 355 nm UVPD and offer different fragmentation pathways. Both derivatization methods upon UVPD yielded [superscript 0,2] A-type ions, however reductive amination and hydrazide conjugation produced dominant [superscript 0,1] A and [superscript 2,4] A-type ions, respectively. Ultraviolet photodissociation at 193 nm (ArF laser, 6.4 eV / photon) has been applied to sialylated oligosaccharides and glycans which were analyzed in negative mode due to their acidic condition. Primarily, UVPD provides a greater array of fragment ions including cross-ring cleavages and dual cleavage internal ions in comparison to CID. In addition, the UVPD generates unique fragment ions which arise from site-specific cleavage of the trial substituent of the sialic acid residue. UVPD of doubly deprotonated sialylated oligosaccharides produced mostly singly deprotonated fragment ions, whereas the product ions in the CID spectra were overwhelmingly doubly charged ions, an outcome attributed to the more extensive cleavages of sialic acid residue upon UVPD. Although electron transfer dissociation (ETD) has shown superior capabilities for the characterization of post-translational modifications of peptides due to its non-eragodic property, ETD has intrinsic drawback arising from its significant dependence on the charge state of the selected precursor ion. Precursor ions in low charge states tend to undergo charge reduction, often preferentially relative to production of the informative cand z-type ions. In order to increase charge states of peptides and ETD efficiencies, peptides were derivatized at their carboxylate groups via attachment of amine with fixed charge or hydrophobic group. The carboxylate-derivatized peptides exhibited higher ETD efficiencies relative to underivatized peptides along with greater numbers of diagnostic fragment ions. The carboxylate derivatization strategy in combination with ETD for proteomics applications by the proteolytic digestion, the derivatization, and LC-MS purification was demonstrated with Cytochrome C.

The "DNA Damage Hypothesis" pertaining to the evolution of sex was tested using Hydra littoralis. DNA damage was produced by irradiating whole live hydra with ultraviolet light. A curve of uv light dosage vs. survival was constructed. Estimations of threshold fluence and LD50 were made from the survival curve. In four separate experiments, using various combinations of environmental temperatures, uv doses, and number of doses, frequencies of asexual and sexual reproduction were observed and compared. The hydra that received uv treatments did not show an increase in the consequent amount of sexual reproduction, and actually showed a decrease. An increase in the amount of sexual reproduction following DNA damage is predicted by the DNA damage hypothesis, therefore these results do not support this theory. The data was also used to make contradictory observations regarding the "stress hypothesis" for the occurrence of sexual reproduction in hydra.

The use of smartphone for the detection of biological constituents is becoming a useful tool as a point-of-care (POC) device and diagnostics. When combined with microfluidic paper analytic devices (μPAD) and particle immunoassays, we have the ability to detect bacterial pathogens with sensitivity and specificity. Environmental conditions as well as variability in smartphone imaging and the cellulose in paper microfluidics however can sometimes easily interfere with the detection of small signal changes. Combining this issue with the detection of pathogens in blood (our model biological sample of interest) becomes difficult with such a platform because of the complexity of the sample matrix. However, in this research we take a novel approach at utilizing polystyrene’s auto-fluorescence and the high energy of UV LEDs in a particle immunoassay in order to increase our signal change. We first characterized how the smartphone actually responds to UV light (275-385 nm) with respect to the RGB components in its images. We were then able to determine a favorable response using the 385 nm UV LED. The detection of green fluorescence by polystyrene particles was possible by analyzing the smartphone’s image in the green channel. There was a significant difference in signal change with blood samples including polystyrene versus just blood samples with a normalized signal intensity change of 2.5 (150%). The detection of polystyrene fluorescence was translated into a field deployable prototype, where preliminary trials showed promising results in detecting Escherichia coli in blood with a current limit of detection of 50 CFU/ml. With further experimentation and optimization the limit of detection could be improved to 10 CFU/mL, making it a very useful tool in the detection of blood borne pathogens to prevent complications with onset bacteremia and the more serious cases of sepsis. This assay platform could provide an easy to use solution with detection in a short time (assay time of 1 min) compared to the lengthy blood culture monitoring or biomarker detection.

The effect of increased stratospheric dust on the polarization and intensity of sunlight scattered by the terrestrial atmosphere in the spectral region near the ultraviolet transmission cutoff is examined. Particular interest is given to radiation reflected to space or transmitted to the surface in the direction perpendicular to the incident solar beam in a plane containing the sun and the local vertical. Theoretical results are presented from radiative transfer calculations using a simple single scattering model and a four layer model in which ground reflection and multiple scattering were treated. Results show that the polarization state of transmitted radiation of .2975 μ for an incident solar zenith angle of 70° is sensitive to dust loading above 50 mb. The depolarization caused by a moderate increase in stratospheric dust loading is found to be roughly comparable to that caused by ground reflection and tropospheric aerosol. The polarization of light reflected to space is found to be very sensitive to high altitude dust scattering while being much less sensitive to other sources of depolarization. Results show that increasing the amount of stratospheric dust can cause either an increase or a decrease in the daily dosage of ultraviolet radiation at the surface, depending on the altitude profile of the dust, the latitude and the season. Preliminary experimental results or polarization monitoring by a ground-based instrument are presented and discussed. Sky light polarization ratios in the direction normal to the solar beam at .2975 μ and .300 μ based on data collected in the spring and summer of 1980 from the roof of the Physics-Atmospheric Sciences building on the campus of The University of Arizona are presented and discussed. The stratospheric dust problem is considered in terms of stratospheric aerosol enhancement resulting from volcanic activity.

No description available.

Cancer is the second leading cause of death in the United States, and its early diagnosis is critical to improving treatment options and patient outcomes. In autofluorescence (AF) imaging, light of controlled wavelengths is projected onto tissue, absorbed by specific molecules, and re-emitted at longer wavelengths. Images of re-emitted light are used together with spectral information to infer tissue functional information and diagnosis. This dissertation describes AF imaging studies of three different organs using data collected from fresh human surgical specimens. In the ovary study, illumination was at 365 nm, and images were captured at 8 emission wavelengths. Measurements from a multispectral imaging system and fiber optic probe were used to map tissue diagnosis at every image pixel. For the colon and pancreas studies, instrumentation was developed extending AF imaging capability to sub-300 nm excitation. Images excited in the deep UV revealed tryptophan and protein content which are believed to change with disease state. Several excitation wavelength bands from 280 nm to 440 nm were investigated. Microscopic AF images collected in the pancreas study included both cultured and primary cells. Several findings are reported. A method of transforming fiber optic probe spectra for direct comparison with imager spectra was devised. Normalization of AF data by green reflectance data was found useful in correcting hemoglobin absorption. Ratio images, both AF and reflectance, were formulated to highlight growths in the colon. Novel tryptophan AF images were found less useful for colon diagnostics than the new ratio techniques. Microscopic tryptophan AF images produce useful visualization of cellular protein content, but their diagnostic value requires further study.