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Prebiotic activity of isomalto-oligosaccharidesKetabi, Ali 06 1900 (has links)
Isomalto-oligosaccharides (IMO) with α(1→6) and α(1→4) glucosidic linkages are produced by enzymatic conversion of starch. Isomalto-oligosaccharides are partially digested in the intestine but little information is available regarding their metabolim in vivo. It was the aim of this study to investigate IMO metabolism by lactobacilli and bifidobacteria and to determine the effect of IMO diet on intestinal microbiota in a rodent model, and a rodent model for inflammatory bowel disease (IBD).
Different strains of bifidobacteria and lactobacilli were grown in de Man Rogosa Sharpe media with IMO as sources of carbohydrates. Substrates and metabolites of carbohydrates metabolism were analyzed. Lactobacilli metabolized isomaltose whereas isomalto-triose and higher degree of polymerisation (DP) oligosaccharides were metabolized by bifidobacteria first. To determine the modulating effects of IMO in the intestine, a group of six F344 rats were fed IMO diet for six weeks and compared to rats fed control diet. Assessment of intestinal microbiota and their metabolites was performed by PCR- denaturing gradient gel electrophoresis (PCR-DGGE), quantitative PCR (qPCR) and quantification of short chain fatty acids (SCFA). The Lactobacillus group was one of the dominant bacterial taxa in the fecal samples of rats. Isomalto-oligosaccharides selectively stimulated lactobacilli and increased their diversity in rats compared to those on control or inulin diet.
The potential health benefit of IMO was evaluated in a rodent model for IBD. Three groups of HLA-B27 rats were fed IMO, fructo-oligosaccharides (FOS) or control diet for 12 weeks. The Lactobacillus group and bifidobacteria numbers were increased significantly in the fecal samples of rats fed IMO or FOS diet respectively. Numbers of Enterobacteriaceae family were significantly increased in the rats fed IMO or FOS diet compared to the control group. Clostridium coccoides group, Clostridium leptum group, Clostridium cluster XI and total number of bacteria were significantly decreased in the rats fed FOS diet compared to the rats fed control diet. Moreover, IMO and FOS diets showed a unique effect on intestinal microbiota compared to the control diet. Cecum histology scores showed a significant decrease of inflammation in the rats fed IMO or FOS diet compared to the rats fed control diet. / Food Science and Technology
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Effects of heparin on 2,4-dinitrobenzene sulfonic acid-induced ulcerative colitis and ethanol-induced gastritis in rats馮漢生, Fung, Hon-sang. January 2000 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Effects of zinc on 2,4-dinitrobenzene sulfonic acid-induced ulcerativecolitis in rats陸皓開, Luk, Ho-hoi. January 2000 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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Prebiotic activity of isomalto-oligosaccharidesKetabi, Ali Unknown Date
No description available.
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The place of counselling in the care of people affected by inflammatory bowel diseaseThomas, Gillian B. January 1996 (has links)
No description available.
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Effects of zinc on 2,4-dinitrobenzene sulfonic acid-induced ulcerative colitis in rats /Luk, Ho-hoi. January 2000 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 73-90).
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Effects of heparin on 2,4-dinitrobenzene sulfonic acid-induced ulcerative colitis and ethanol-induced gastritis in rats /Fung, Hon-sang. January 2000 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 60-75).
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Effects of heparin on 2,4-dinitrobenzene sulfonic acid-induced ulcerative colitis and ethanol-induced gastritis in ratsFung, Hon-sang. January 2000 (has links)
Thesis (M.Med.Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 60-75). Also available in print.
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Effects of zinc on 2,4-dinitrobenzene sulfonic acid-induced ulcerative colitis in ratsLuk, Ho-hoi. January 2000 (has links)
Thesis (M.Med.Sc.)--University of Hong Kong, 2000. / Includes bibliographical references (leaves 73-90). Also available in print.
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Immunologyical mechanisms in primary primary sclerosing cholangitis : the role of the anti-neutrophil cytoplasmic antibodyBansi, Devinder Singh January 1997 (has links)
No description available.
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