This study focuses on how and why people become ready to interact with certain information systems (IS) based on their previous experiences with the same and/or similar systems. User-system interaction can be regarded as a mediated and collaborative human activity between a user and a system with the motive of transforming raw information into useful outcome. Using Activity Theory as a paradigm, this study conceptualizes a user-system interaction model that specifies the mediating relationships involved. Based on the user-system interaction model, this study proposes a psychological construct, Information System Interaction Readiness (ISIR), that indicates how an individual is prepared and willing to interact with a system within a user context. This construct advances a developmental view of how previous IS experiences may affect user future behavior. Compared with other constructs as predictors of user behavior, such as computer self-efficacy and intention to use, ISIR takes how IS user behavior is mediated into account. To operationalize and measure the ISIR construct, this study develops a measurement instrument for ISIR using the technique of facet analysis and the semantic differential scale type. To explore how user experiences with a system lead to the formation of ISIR, this study identifies the psychological antecedents of ISIR. This enables the discussion of how general IS capabilities, including interactivity, personalization and context-awareness, may affect ISIR through these antecedents. Because ISIR is a user-, system- and context-specific construct, this study also identifies and discusses the personal and situational factors that may affect ISIR. Putting all these relationships together results in a research framework of ISIR. To validate the ISIR measurement instrument and test the ISIR research framework, several laboratory studies were conducted. The results indicated that the ISIR instrument was valid and the ISIR framework was sound. Finally, the contributions and limitations of this study are discussed.