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This work describes an implementation of an iterative algorithm to design two-zone binary phase diffractive optical elements (DOEs) which produce a specific irradiance distribution in both the axial and transverse directions under high numerical aperture focusing. The full vector character of a polarized beam is rigorously accounted for using the vector diffraction integrals. The axial and tran verse irradiance distributions are calculated for applications involving multiphoton direct la er writing, specifically, a novel method known as resolution augmentation through photo induced deactivation or, RAPID. In the RAPID method two beams are used, one for excitation and the other for de-excitation. By having both the excitation and de-excitation beams, the resolution achievable using the RAPID method is maximized.

In the age where quick turn around time and high speed manufacturing methods are becoming more important, quality assurance is a consistent bottleneck in production. With the development of cheap and fast computer hardware, it has become viable to use machine vision for the collection of data points from a machined part. The generation of these large sample points have necessitated a need for a comprehensive algorithm that will be able to provide accurate results while being computationally efficient. Current established methods are least-squares (LSQ) and non-linear programming (NLP). The LSQ method is often deemed too inaccurate and is prone to providing bad results, while the NLP method is computationally taxing. A novel method of using support vector regression (SVR) to solve the NP-hard problem of cylindricity of machined parts is proposed. This method was evaluated against LSQ and NLP in both accuracy and CPU processing time. An open-source, user-modifiable programming package was developed to test the model. Analysis of test results show the novel SVR algorithm to be a viable alternative in exploring different methods of cylindricity in real-world manufacturing.

The coat protein of satellite panicum mosaic virus (SPMV) was used to stabilize viral vector gene inserts in planta. A Potato virus X (PVX) vector carrying the SPMV capsid protein (CP) gene was successfully stabilized through three serial passages in Nicotiana benthamiana from the upper non-inoculated leaves following rub inoculation. The presence of SPMV CP expression from the PVX vector was confirmed by necrotic lesions that occur only when SPMV CP is present and by western blot and reversetranscription PCR analyses. In addition, PVX-SPCP was co-inoculated onto N. benthamiana with a Tomato bushy stunt virus vector carrying a green fluorescent protein gene, which normally does not yield GFP expression in upper tissue due to loss of the insert. However, upon co-inoculation with PVX-SPCP, upper non-inoculated leaves exhibited GFP accumulation based on green fluorescence by UV illumination at 488 nm and western blot analysis. GFP expression was more abundant in upper non-inoculated N. benthamiana leaves as well as systemic tissues when the co-inoculation experiments were performed at 20°C compared to 25°C. These results suggest that SPMV CP is a viable molecular tool for stabilizing viral vector gene inserts in planta.

The purpose of this work is to evaluate the ratio of the vector and axial vector coupling constants in the beta-decay of Rb^87. Particular attention is given to determining the sign of the ratio. All this is accomplished mainly by a conic analysis of the beta-spectrum and the calculation of a matrix element ratio. The results obtained indicate that the ratio of the coupling constants is negative. Due to uncertainties in nuclear matrix elements, only an approximate absolute value for the ratio can be secured. These results are consistent with other present evidence. / Thesis / Master of Science (MSc)

Lorentz TEM observations of magnetic nanoparticles contain information on the magnetic and electrostatic potentials of the sample. These potentials can be extracted from the electron wave phase shift by separating electrostatic and magnetic phase shifts, followed by 3D tomographic reconstructions. In past, Vector Field Electron Tomography (VFET) was utilized to perform the reconstruction. However, VFET is based on a conventional tomography method called filtered back-projection (FBP). Consequently, the VFET approach tends to produce inconsistencies that are prominent along the edges of the sample. We propose a model-based iterative reconstruction (MBIR) approach to improve the reconstruction of magnetic vector potential, A(r). In the case of scalar tomography, the MBIR method is known to yield better reconstructions than the conventional FBP approach, due to the fact that MBIR can incorporate prior knowledge about the system to be reconstructed. For the same reason, we seek to use the MBIR approach to optimize vector field tomographic reconstructions via incorporation of prior knowledge. We combine a forward model for image formation in TEM experiments with a prior model to formulate the tomographic problem as a maximum a posteriori probability estimation problem (MAP). The MAP cost function is minimized iteratively to deduce the vector potential. A detailed study of reconstructions from simulated as well as experimental data sets is provided to establish the superiority of the MBIR approach over the VFET approach.

Arthropod-borne viruses, arboviruses, have the ability to replicate in both vertebrates and invertebrates and are transmitted to susceptible vertebrate hosts by vectors such as mosquitoes and ticks. Ticks are important vectors of many highly pathogenic arboviruses, including the flavivirus tick-borne encephalitis virus (TBEV) and the nairovirus Crimean-Congo haemorrhagic fever virus. In contrast, alphaviruses are principally mosquito-borne and have been isolated only rarely from ticks; ticks have not been implicated as their vectors. Nevertheless, the alphavirus Semliki Forest virus (SFV) replicates in cell lines derived from many different tick species, including those of the genus Ixodes, which includes vectors of TBEV and its lesspathogenic relative Langat virus (LGTV). In vertebrate cells, arboviruses generally cause cytopathic effects; however, arbovirus infection of arthropod cells usually results in a persistent low-level infection without cell death. While little is known about antiviral immunity in tick cells, the immune system of other arbovirus vectors such as mosquitoes has been studied extensively over the last decade. In insects, pathways such as RNA interference (RNAi), JAK/STAT, Toll, Imd and melanisation have been implicated in controlling arbovirus infection, with RNAi being considered the most important antiviral mechanism. In tick cells, RNAi has been shown to have an antiviral effect, but current knowledge of other immunity pathways is limited and none have been implicated in the antiviral response. In the present study, SFV and LGTV replication in selected Ixodes spp. tick cell lines was characterised and the Ixodes scapularis-derived cell line IDE8 was identified as a suitable cell line for this project. Potential antiviral innate immunity pathways were investigated; putative components of the tick JAK/STAT, Toll and Imd pathways were identified by BLAST search using available sequences from well-studied arthropods including the fruit fly Drosophila melanogaster. Using gene silencing, an attempt was made to determine whether these pathways play a role in controlling SFV and LGTV infection in tick cell lines. Selected genes were silenced in IDE8 cells using long target-specific dsRNA and cells were subsequently infected with either SFV or LGTV. Effects of gene silencing on virus replication were assessed by quantitative real time PCR (qPCR) or luciferase reporter assay. Effects on infectious virus production were measured by plaque assay. Replication of the orbivirus St Croix River virus (SCRV), which chronically infects IDE8 cells, was also quantified by qPCR after silencing of selected genes. Interestingly, SFV or LGTV infection of IDE8 cells resulted in a significant increase in SCRV replication, possibly as a result of interference with antiviral pathways by SFV and LGTV or possibly due to diversion of cellular responses from sole control of SCRV. No evidence for an antiviral role for the JAK/STAT or Toll pathways was found in IDE8 cells. However, an antiviral effect was observed for protein orthologues putatively involved in the RNAi response. Argonaute proteins play an important role in translation inhibition and target degradation mediated by RNAi, and silencing of selected Argonaute proteins resulted in a significant increase in SFV and SCRV replication. The carboxypeptidase CG4572 is essential for an efficient antiviral response in D. melanogaster, and supposedly involved in the systemic RNAi response. A putative tick orthologue of CG4572 was identified and this appeared to be involved in the antiviral response in IDE8 tick cells. When expression of CG4572 was silenced and cells subsequently infected with SFV or LGTV, replication of both viruses was significantly increased. In addition, it was shown that three mosquito orthologues of CG4572 also had an antiviral role against SFV in Aedes mosquito cells. In conclusion, of the tick cell lines investigated, IDE8 provided a suitable model system for investigating tick cell responses against arboviruses and new insight into the nature of the tick cell antiviral response was gained.

Hyper-wideband communications represent the next frontier in spread spectrum RF systems with an excess of 10 GHz instantaneous bandwidth. In this thesis, an end-to-end physical layer link is implemented featuring 16k-OFDM with a 4 GHz-wide channel centered at 9 GHz. No a priori channel state information is assumed; channel information is derived from the preamble and comb pilot structure. Due to the unique expansive spectral properties, the channel estimator is primarily composed of least squares channel estimates combined with a robust support vector statistical learning approach using autonomously selected parameters. The system’s performance is demonstrated through indoor wireless experiments, including line-of-sight and near-line-of-sight links. Moreover, it is shown that the support vector approach performs superior to linear and cubic spline inter/extrapolation of the least squares channel estimates.

International Telemetering Conference Proceedings / October 30-November 02, 1995 / Riviera Hotel, Las Vegas, Nevada / Video compression is typically required to solve the bandwidth problems related to the transmission of instrumentation video. The use of color systems typically results in bandwidth requirements beyond the capabilities of current receiving and recording equipment. The HORACE specification, IRIG-210, was introduced as an attempt to provide standardization between government test ranges. The specification provides for video compression in order to alleviate the bandwidth problems associated with instrumentation video and is intended to assure compatibility, data quality, and performance of instrumentation video systems. This paper provides an overview of compression methods available for instrumentation video and summarizes the benefits of each method and the problems associated with different compression methods when utilized for instrumentation video. The affects of increased data link bit error rates are also discussed for each compression method. This paper also includes a synopsis of the current HORACE specification, a proposed Vector HORACE specification for color images and hardware being developed to meet both specifications.

published_or_final_version / Nursing Studies / Master / Master of Nursing