Work includes certification procedures for experimental aeroplanes. Producer must answer individual work for right procedure certification. Office UCL give works and control production procedure aeroplane. Stationery (applications, confirmation, tec.) are part of work and is need make and feed. Part of work is VUT 001 MARABU and technical characteristics aeroplane.

>Magister Scientiae - MSc / In the standard (‘concordance’) model of Cosmology, there is a fundamental assumption that the Universe is statistically isotropic and homogeneous on large scales, known as the Cosmological Principle. The Cosmological Principle requires that the dipole anisotropy apparent in the CMB should also be observed in galaxy number counts if this signal occurs due to the aberration and Doppler effects from our peculiar motion. This thesis will investigate the accuracy with which the cosmic kinematic dipole can be determined by comparing real data from NRAO VLA Sky Survey (NVSS) catalog with the simulated sky maps following its specifications. The mock maps are generated using FLASK code which assumes a lognormal distribution for the radio count density field from z=0 to z =4 and taking as an entry an angular power spectrum from CAMB which assumed a flat ΛCDM cosmology and a redshift distribution. After analising the kinematic dipole, we turn to the analysis of statistical isotropy in the catalog. We used ANalysis Of Variance (ANOVA) test on patches in the sky of different radii as one of the statistical tools for the analysis. We found that as we go to a higher radius for the patches, we have a better agreement between the theory and the observation as expected. We also saw that the more we are rigorous on the rejection criteria, the smaller is the discrepancy between the observed and simulated number count distribution in the sky. We found an optimum choice of 25◦ as patch size, and if the accepted patches have a maximum of 30% of their pixels masked. Therefore, we find that the NVSS data agrees with the fundamental assumption of statistical isotropy at angular scales > 20◦.

Submitted by VANESSA JORDÃO MARCATO FERNANDES null (vanessamarcato@yahoo.com.br) on 2017-01-30T18:15:29Z No. of bitstreams: 1 fernandes_vjm_tese.pdf: 25329126 bytes, checksum: 92c837eb39ae9af74c15ccf278cd2a84 (MD5) / Approved for entry into archive by LUIZA DE MENEZES ROMANETTO (luizamenezes@reitoria.unesp.br) on 2017-02-03T16:39:11Z (GMT) No. of bitstreams: 1 fernandes_vjm_dr_prud.pdf: 25329126 bytes, checksum: 92c837eb39ae9af74c15ccf278cd2a84 (MD5) / Made available in DSpace on 2017-02-03T16:39:11Z (GMT). No. of bitstreams: 1 fernandes_vjm_dr_prud.pdf: 25329126 bytes, checksum: 92c837eb39ae9af74c15ccf278cd2a84 (MD5) Previous issue date: 2016-12-19 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Esse trabalho propõe o desenvolvimento de um método para a extração automática de contornos de telhados de edifícios com a combinação de dados de Varredura a LASER Aerotransportado (VLA) e dados fotogramétricos e campos aleatórios de Markov (MRF). Inicialmente, um Modelo Digital de Superfície normalizado (MDSn) é gerado através da diferença entre o Modelo Digital de Superfície (MDS) e o Modelo Digital de Terreno (MDT), obtidos a partir da nuvem de pontos LASER. Em seguida, o MDSn é segmentado para a obtenção dos polígonos que representam objetos altos da cena. Esses polígonos são projetados na imagem para restringir o espaço de busca para a segmentação da imagem em regiões. Esse processo possibilita a extração de polígonos na imagem que representem objetos altos. O processo de identificação de contornos de telhados, em meio aos objetos altos detectados na imagem, na etapa anterior, é realizado através da otimização de uma função de energia estabelecida com base em MRF que modela propriedades específicas de contornos de telhados de edifícios. No modelo MRF são utilizados tanto os polígonos extraídos da imagem quanto os extraídos dos dados VLA. A função de energia é otimizada pelo método Algoritmo Genético (AG). O método proposto nesse trabalho foi avaliado com base em dados reais - imagens aéreas de alta resolução e dados VLA. Os resultados obtidos na avaliação experimental mostraram que a metodologia funciona adequadamente na tarefa de extrair os contornos de telhados de edifícios. A função de energia proposta associada ao método de otimização AG diferenciou corretamente os contornos de telhados de edifícios dos demais objetos altos presentes nas cenas. Os contornos de telhados extraídos apresentam boa qualidade, o que é evidenciado por meio dos índices de completeza e correção obtidos pela avaliação numérica. Com base nos índices médios obtidos para cada experimento, têm-se as médias de completeza e correção para os experimentos iguais a 90,96% e 98,99%, respectivamente. Os valores máximos de completeza e correção são de 99,19% e 99,94%, respectivamente, e os valores mínimos de 78,08% e 97,46%, respectivamente. Os menores valores de completeza estão associados às áreas de oclusão por vegetação e presença de sombras. / This paper proposes a method for the automatic extraction of building roof contours through a combination of Airborne Laser Scanner (ALS) and photogrammetric data, and Markov Random Field (MRF). Initially, a normalized digital surface model (nDSM) is generated on the basis of the difference between the digital surface model and the digital terrain model, obtained from the LiDAR point cloud. Then the nDSM is segmented to obtain the polygons representing aboveground objects. These polygons are projected onto image to restrict the search space for image segmentation into regions. This process enables the extraction of polygons in the image representing aboveground objects. Building roof contours are identified from among the aboveground objects in the image by optimizing a Markov-random-field-based energy function that embodies roof contour specific properties. In the MRF model are used both polygons extracted from image and from ALS data. The energy function is optimized by the Genetic Algorithm (GA) method. The method proposed in this work was evaluated based on real data - high-resolution aerial images and ALS data. The results obtained in the experimental evaluation showed that the methodology works adequately in the task of extracting the contours of building roofs. The proposed energy function associated with the GA optimization method correctly differentiated the building roof contours from the other high objects present in the scenes. The extracted roof contours show good quality, which is evidenced by the indexes of completeness and correctness obtained by numerical evaluation. Based on the mean indexes obtained for each experiment, the average completeness and correctness for the experiments were equal to 90.96% and 98.99%, respectively. The maximum completeness and correctness values are 99.19% and 99.94%, respectively, and the minimum values are 78.08% and 97.46%, respectively. The lowest values of completeness are associated to the vegetation occlusion areas and presence of shadows. / FAPESP: 2012/22332-2

The chemokine, Monocyte Chemoattractant Protein (MCP-1), enhances integrin mediated monocyte adhesion to the vascular endothelium during inflammation. In this study, we demonstrate that MCP-1 promotes rapid sub-second adhesion of THP-1 cells to Vascular Cell Adhesion Molecule-1 (VCAM-1), but not to Intercellular Cell Adhesion Molecule-1 (ICAM-1). MCP-1 activates membrane tethered Very Late Antigen 4 (VLA-4, α4β1), but not necessarily cytoskeleton anchored VLA-4. Activated tethered VLA-4 bonds tremendously increased the period of time monocytes remain bound from hundreds of milliseconds to several seconds and also increased the distance over which immunologic surveillance occurs from several microns up to 20 microns along the endothelium. Lastly at the single molecule level, MCP-1 stimulated tethered VLA-4 bonds exhibit increased resistance to pulling force. In conclusion MCP-1 increased tethered VLA-4 bond resistance to force providing a mechanism for monocyte recruitment to the endothelium.

Le rôle central de VLA-4 et de CXCR4 dans le processus dimplantation médullaire des cellules souches hématopoïétiques (CSH) est bien documenté tandis que la fonction de VLA-5 est plus discutée. Plusieurs études ont rapporté que lamplification ex vivo des CSH en présence de cytokines exogènes réduisait leurs capacités de repeuplement médullaire ; le maintien dune interaction appropriée dans lenvironnement médullaire étant critique pour la conservation dune implantation efficace. Les travaux présentés contribuent à lidentification des changements fonctionnels des récepteurs dadhérence impliqués dans le processus implantatoire des CSH cultivées. En première partie, les interactions statiques et dynamiques de LTC-IC générées ex vivo vis-à-vis de ligands du stroma médullaire ont été comparées aux LTC-IC natives. Lévaluation de ladhérence des LTC-IC in vitro sur les fragments de Fn et VCAM-1 a permis didentifier une altération de létat dactivité de VLA-4 et VLA-5 après expansion. Les adhérences statiques et dynamiques médiées par VLA-4 sur VCAM-1 et Fn étaient compromises contrairement au VLA-5 dont lactivité était augmentée après expansion. Dans un second temps, leffet de lexposition cytokinique sur la contribution respective de VLA-4, VLA-5 et CXCR-4 dans limplantation et le repeuplement médullaire a été examinée chez la souris NOD/SCID et NOD/SCID b2-/-. Il a été montré que limplantation et le repeuplement par des CSH natives sont dépendants de VLA-4, lexpansion ex vivo étant associée à linactivation de VLA-4 ce qui révèle le rôle joué par VLA-5 dans la reconstitution hématopoïétique in vivo. De plus, une diminution de lactivité de CXCR-4 peut induire une perte de sélectivité médullaire lors de limplantation de CSH amplifiées.

In our previous work we demonstrated that the integrin antagonist, HYD1, induced necrotic cell death in myeloma cell lines in vitro and in vivo as a single agent. In order to further delineate biomarkers of response to HYD1 we developed an isogenic drug resistant variant named H929-60. We show that the acquisition of resistance towards HYD1 correlates with reduced expression of the cleaved α4 integrin subunit and beta 1 integrin. Moreover, we demonstrate that HYD1 interacts with α4 integrin in myeloma cells. Consistent with reduced VLA-4 expression, the resistant variant showed ablated functional binding to fibronectin, VCAM-1 and the bone marrow stroma cell line, HS-5. The reduction in binding to extracellular matrices of the resistant variant translated to sensitivity to melphalan and bortezomib induced cell death in the bone marrow stroma co-culture model of drug resistance. Moreover, CD138 positive myeloma cells were more sensitive to HYD1 induced cell death compared to the CD138 negative fraction, and potency of HYD1 induced cell death significantly correlated with α4 integrin expression. We were also able to show that reducing α4 or β1 integrin using shRNA strategies was sufficient to cause resistance in myeloma cell lines. In addition we investigated the effects of cyclized variants of HYD1 to improve potency of the agent. One such compound, named HM-27, was determined to be 30 fold more active in H929 cells when compared to HYD1. HM-27 and HYD1 were determined to have similar mechanisms of action as H929-60 cells were shown to be resistant to both compounds when compared to H929 cells. We further characterized HM-27's mechanism of action by investigating what effects HM-27 induced Ca2+ oscillations had on HM-27 induced cell death. The increases in intracellular Ca2+ seen after treatment with HM-27 were determined to occur via release from ER stores and not through influx through plasma membrane channels. Inhibiting Ca2+ release from the ER also potentiated the effects of HM-27 in MM cells. Furthermore, inhibiting Ca2+ release from the ER was also shown to block the onset of autophagy after ER treatment. Treating cells with the lysosomotropic agent, chloroquine, was shown to potentiate the activity of HM-27 in vitro and ex vivo. HM-27 was also shown to have activity in an in vivo model with combination treatment containing bortezomib and HM-27 increasing mouse survival. Collectively our data indicate that VLA-4 expression is a critical determinant of response to HYD1 induced cell death. We also showed that increases in intracellular Ca2+ seen after treatment with HM-27 had a cytoprotective effect in MM cells. Moreover, neutralizing autophagy potentiates HM-27 induced cell death in vitro and ex vivo while combining bortezomib and HM-27 increased survival in vivo. These data continue to provide rationale for further pre-clinical development of HYD1 as a novel anticancer agent.

This thesis is aimed at investigating the possibilities of using vertical line array (VLA) data to image the gas hydrate stability zone (GHSZ) in the northern Gulf of Mexico. The presence of gas hydrate can be inferred from seismic evidence such as bottom simulating reflectors (BSRs) or changes in seismic velocity. The petroliferous northern Gulf of Mexico is noted for its obvious absence of BSRs, a characteristic it shares with other active passive margins with mobile salt and/or shale, which have high propagation velocities for seismic waves. This makes the imaging and the identification of the gas hydrates a challenging process with conventional seismic techniques. Therefore. new techniques in data acquisition. processing and analysis are sought to improve the imaging of complex areas. The new, unconventional seismic data acquisition technique used here is the VELA. This work defines a seismic processing flow that has been developed to extract velocity, travel-time and amplitude information from VLA data to predict the hydrate distribution over the surveyed area. Specialized amplitude versus offset analysis and inversion is applied to the VLA data using a Bayesian inversion approach to provide estimates and uncertainties of the viscoelastic physical parameters at an interface. This thesis will compare the inversion of the 2-D seismic reflection data collected in 1998 by the USGS and in 2002 by the Center for Marine Resources and Environmental Technology (CMRET) to the VLA data collected in 2002 and 2003 by the CMRET to assess the value of a VLA in monitoring changes in the near-surface sediments that can be associated with the presence of gas hydrate.

No description available.

This thesis is focused on a project of all metal aircraft by UL-2 and LSA rules. The background research of aircrafts in existing category has been made in the market. The concept, with whom this thesis deals with, has been projected thanks to this analysis. The second part of the thesis is focused on avionics equipments of aircrafts. Market analysis has been made and the way of medernizing small sports airfts' equipment in categories UL and LSA has been projected.

Radio Astronomy, the study of distant objects in the radio spectrum, is set at defiance against all other users of the electromagnetic spectrum. Where traditional use would fill the electromagnetic spectrum with as much transmission and information as possible, Radio Astronomy would rather no man-made signals existed. Since that is not possible, they grumble and have to deal with unwanted transmissions impinging upon their instruments. I have demonstrated that subspace projection can remove these unwanted signals from Radio Astronomical data in post processing. I demonstrate it both on simulated data and on data taken from the Very Large Array radio telescope.In the process of implementing the algorithm, I show that the self power terms contain necessary information about the array element responses to RFI sources. While the autocorrelation are not used in the normal synthesis imaging process, my work shows that RFI mitigation using Subspace Projection performs better with the autocorrelations retained when computing projection matrices. Furthermore, I demonstrate that proper data collection allows a significant decrease in error under subspace projection. Potential enhancements to subspace projection are also briefly considered.