D.Phil. (Zoology) / Fish parasites may cause disease and lead to commercial losses. In order to construct practical management systems to mitigate or manage potential adverse effects, information on basic biological variables is required. Compared to the number of internationally published papers on the subject, little is known about the monogenean parasite fauna of South African fishes. Monogeneans are largely ectoparasitic, thin, flattened, host- and site specific parasites with a simple life cycle involving a single host (often a fish). They range in size between 0.3 mm and 20 mm and are mostly bilaterally symmetrical with the body subdivided into a number of regions. Attachment organs are a necessary feature both anteriorly and posteriorly, with the morphology of the prominent posterior attachment organ (opisthaptor) highly variable between genera. It may contain suckers, clamps or large hooks (anchors) as well as marginal hooks. In some genera anchors (one to two pairs) are associated with spikes or accessory sclerites and are supported by a connecting bar. All these structures, together with the sclerotized male copulatory organ, are of taxonomic significance....

M.Sc. / The Vaal River is the main source of water supply to the central industrial, mining and metropolitan regions of South Africa, and is, therefore, strictly regulated by small dams and weirs. The Vaal Dam is the main regulator of water to the Vaal River and is of great importance as it supplies water for human consumption and also to the industrial powerhouse of South Africa. Situated at the confluence of its major tributaries, namely the Vaal and Wilge Rivers, and straddling the convergence of the borders of Gauteng, Free State and Mpumalanga provinces, the Vaal Dam is approximately 77 km south of Johannesburg. It is South Africa’s second-largest dam in terms of area, and third-largest in terms of volume, and is a key component in South Africa’s water supply infrastructure. Gauteng, as well as the surrounding provinces are reliant on it for their water supply. Water flow fluctuations are deemed to be important as they could negatively impact upon the water quality. Knowledge of the relationship between fluctuating water flows and water quality is important as strategies can then be devised on this basis to improve the freshwater situation of the country, the associated management systems, and treatment technologies. By establishing the nature of the respective relationships between high water flow and the selected water quality parameters, ways could be found of reducing the costs of water quality problems, such as eutrophication. The various relationships between high water flow and the selected water quality parameters of the Vaal Dam that were established during the course of this study are as follows: In terms of pH, the study found that in the event of a decline in the water flow, the pH value increased and the water body became more alkaline. A decrease in water flow was also found to be associated with an increase in EC. An increase in water flow was found to be associated with a decrease in pH, EC and Chlorophyll-a. An increase in water flow up to a certain level was found to be associated with an increase in nitrates, sulphates and COD.

M.Sc. (Zoology) / The world’s ever increasing human population has lead to an almost unimaginable amount of waste being released in to the aquatic environment every day. Aquatic systems are faced with the greatest risk due to the fact that water is an indispensable resource required for industrial and agricultural processes. In recent years there has been a dire need for the monitoring and rehabilitation of aquatic systems. As a result many biological monitoring programmes were set into place in an attempt to manage this problem. The use of aquatic organisms as sentinels for biomonitoring studies has been wildly accepted with the majority of biomonitoring research focusing on the use of various fish and invertebrate species as aquatic sentinel organisms. However, the use of parasites as sentinel organisms is a relatively new field and as a result there has been little published work on the use of monogenean ectoparasites parasites as such sentinels. The bioaccumulation and subsequent biomagnification of metals by certain parasite species is well known, with bulk of the published work focusing on endoparasites (cestodes, acanthocephalans & nematodes), these published studies indicate that some species of endoparasites exhibit a remarkable ability to biomagnify metals in concentrations that far exceed that of their respective hosts as well as the ambient environment. Thus this project aims to assess the bioaccumulation and biomagnification of metals by the ectoparasitic diplozoon. The study site that was chosen for this project was the Vaal Dam; this site was deemed appropriate due to its near pristine condition and major economic importance. This meant that this particular site is ideal for the testing of a new biomonitoring system. All field sampling was performed around UJ Island (26°52.249’S; 20°10.249’E) from February 2011 to April 2011. A total of 29 Labeo umbratus (Smith, 1841) were collected with the aid of gill nets and three sediment samples were also taken using a grab sampler. Water quality data was obtained from the Rand Water Analytical Facility in Vereeniging as this organisation routinely monitors of the water quality parameters and the metal concentrations within the surface waters of the Vaal Dam, this information was obtained with the aid of data loggers situated in the dam...

M.Sc. / There are 39 known species of Lamproglena to date. A review of all species including the species name, the author, as well as an accompanying sketch and information regarding the distribution, pathology and host preference is given. Lamproglena clariae is a copepod gill parasite found on Clarias gariepinus. It has been observed that they cause extensive gill tissue proliferation, which may interfere with respiration. It is uncertain on which tissue type Lamproglena feeds, as red blood is never apparent in the gut of Lamproglena monodi, whereas in the cases of both L. clariae and L. barbicola they undoubtedly feed on blood, proven by the frequently blood-filled gut. Even thought the mouthparts are not fully understood as yet, it is deduced that feeding involves only the maxillae and maxillipedes. Many poecilostomes lacks mandibles. Also, in the lernaeids the mandible is appendage is the smallest and from the 39 known Lamproglena species, the mandibles are described in only eight species. This study examines the digestive tract and the structure of the mouthparts using both light and scanning electron microscopy and tracts the nerves to establish if L. clariae does possess mandibles. Specimens were collected from Clarias gariepinus in the Vaal Dam after the fish were euthanised and the gills removed. The samples were fixed in an aceto formaldehyde alcohol solution and preserved in 70% ethanol prior to dehydration and embedding in resin. Serial section were made at 5 pm and stained with a trichrome stain. A schematic as well as graphic reconstruction of the digestive system is presented. The digestive system consist of three predominant parts, the fore, mid and hindguts. The foregut has a cuticularised epithelium. The oesophagus has a muscle layer iv which stretched and contracts in order to aid feeding and it extends into the midgut via a funnel-like structure. There are four designated zones within the midgut. The hindgut is a simple, cuticle lined tube. Cell structures are discussed. The bilobed protocerebrum in L. clariae is present. The deutocerebrum innervates the antennules. The tritocerebrum consist of two lobed parts and innervates the antennules. The ventral nerve cord is situated posterior to the cephalon. It protrudes dorsally, from where the posterior commissures innervates. The anterior commissures innervates closer to the tritocerebrum. The nerves leading to the mandibles are innervated from the anterior commissure. The more posteriorly located commissure innervates the nerve to the seta located between the antennae and the maxillae. Both the nerves to the maxillae and the endopodite to the maxillae are innervated from this commissure. Mandibles were left out of previous species descriptions of L. clariae. Other Lamproglena species descriptions, showing the mandibles, revealed slender looking mandibles located in close proximity to the mouth opening. This is contrary to the stout mandibles of L. clariae. Future research topics are highlighted and discussed

Prof. A. Avent-Oldewage

Submitted in fulfillment of the requirements of the Degree of Master of Technology: Chemistry, Durban University of Technology, 2014. / This project arose out the need for a simple method to analyse NOM on a routine basis. Water samples were obtained from the Vaal dam, which is one of the dams used by a hydroelectric power station. Analysis was preceded by separation of NOM into the humic and non-humic portions. The humic portion was separated into two fractions by employing a non-ionic resin (DAX-8) to separate humic acid from fulvic acid. High performance size exclusion chromatography (HPSEC), equipped with an Ultraviolet( UV) detector and an Evaporative Light Scattering (ELS) detector connected in series, was used to obtain molecular weight distribution information and the concentration levels of the two acids. Mixed standards of polyethylene oxide/glycol were employed to calibrate the selected column. Suwanee River humic acid standard was used as a certified reference material. The molecular weight distributions (MWDs) of the isolated fractions of humic and fulvic acids were determined with ELSD detection as weight-average (Mw), number-average (Mn) and polydispersity (ρ) of individual NOM fractions. The Mw/Mn ratio was found to be less than 1.5 in all the fractions, indicating that they have a low and narrow size fraction. An increase in Mn and Mw values, with increasing wavelength for all three humic substances (HS) examined was observed. The HS, isolated from the dam water, was found to be about the same molecular weight as the International Humic Acid Standard (IIHSS). For the fulvic acid standard, the molecular weight was estimated to be around 7500 Da. Characterization of NOM was done to assist in the identification of the species present in the water. FTIR-ATR was used to as a characterization tool to identify the functional groups in the structure of the humic and fulvic acid respectively present in the Vaal Dam. Analysis of the infrared (IR) spectra indicated that the humic acids of the Vaal dam have phenolic hydroxyl groups, hydroxyl groups, conjugated double bond of aromatic family (C=C), and free carboxyl groups. The isolation method has proved to be applicable and reliable for dam water samples and showed to successfully separate the humic substances from water and further separate the humic substances into its hydrophobic acids, namely, humic and fulvic acids. It can be concluded that the Eskom Vaal dam composes of humic substance which shows that the technique alone gives a very good indication of the characteristics of water. The HPSEC method used, equipped with UV and ELSD was able to identify the molecular weight range of NOM present in source water as it confirmed that the Eskom Vaal dam contains humic substances as humic acid and fulvic acid and these pose a health concern as they can form disinfectant byproducts in the course of water treatment with chemicals. FTIR characterization was successful as important functional groups were clearly assigned. Lastly, the use of the TOC and DOC values to calculate SUVA was also a good tool to indicate the organic content in water. It is recommended to use larger amounts of water must be processed to obtain useful quantities of the humic and fulvic acid fractions.

The relationship between Man and Nature can be divided into three ‘eras.’ The first era refers to a time when Man was dominated by Nature, governed by her fluctuating seasons and at the mercy of her erratic moods. Man’s advancement brought into being the Machine, which enabled Man to move into a second era. This era brought about a brief state of equilibrium between Nature and Man, before Man advanced even further and faster into a third era in which Man and Machine dominated Nature. Man’s appropriation of Nature’s resources is his most intimate experience of the Natural world. However, in this third phase of development, Man has negated the ‘natural’ source of Nature’s resources and has thus disconnected himself from her. As a result of this disconnection, the third era is characterised by an imbalance which is leading towards the possibility of Man tipping off the scales entirely. This thesis explores the possibility of a fourth era in which Man and Nature once again achieve an equilibrium of mutually beneficial symbiosis. A fourth era would be the setting of a mend in the severed Man-Nature relationship. A ‘reconnection’ could be achieved through a renewed understanding of the systems, both natural and technological, that supply Man with Nature’s resources. The ‘Machine’ that is the Vaal Dam Wall and its surrounds was chosen as a locus for the theoretical application of a form of inhabited resource infrastructure that takes steps towards a fused Man-Nature entity. The dam wall is a significant piece of existing infrastructure that plays a major role in the capture, storage, and distribution of water to people in Gauteng, South Africa’s economic powerhouse. A legible, multi-use intervention sensitively placed on this site could reconnect the water-users to the natural water resource that is so vital to their livelihoods. The intervention is in the form of a visitor centre which incorporates water purification and hydroelectric power generation as well as a management facility for the Department of Water Affairs, the entity with jurisdiction over the Vaal water system and dam wall site. The Vaal Dam Visitor Centre could provide much-needed infrastructure to the chosen site in its un-realised capacity as a locus for tourism, education, research and management. In doing so, the Centre could be the ‘Machine’ that reconnects Man to Nature through responsible use and understanding of her resources.

M. Tech. (Chemical Engineering, Faculty of Engineering and Technology), Vaal University of Technology. / A novel technology in the treatment of river water, which involves an electrochemical treatment technique to produce domestic or drinking water is being investigated using aluminium and iron electrodes in an electrochemical circuit. Coagulation and flocculation are traditional methods for the treatment of polluted water. Electrocoagulation presents a robust novel and innovative alternative in which a sacrificial metal anode treats water electrochemically. This has the major advantage of providing mainly active cations required for coagulation and flocculation, without increasing the salinity of the water. Electrocoagulation is a complex process with a multitude of mechanisms operating synergistically to remove pollutants from the water. A wide variety of opinions exist in the literature for key mechanisms. A lack of a systematic approach has resulted in a myriad of designs for electrocoagulation reactors without due consideration of the complexity of the system. A systematic, holistic approach is required to understand electrocoagulation and its controlling parameters. An electrocoagulation-flotation process has been developed for water treatment. This involved an electrolytic reactor with aluminium and/or iron electrodes. The water to be treated (river water) was subjected to coagulation, by Al(III) and Fe(II) ions dissolved from the electrodes, resulting in floes floating after being captured by hydrogen gas bubbles generated at the cathode surfaces. Apparent current efficiencies for AI and Fe dissolution as aqueous Al(III) and Fe(II) species at pH 6.5 and 7.8 were greater than unity. This was due to additional chemical reactions occurring parallel with electrochemical AI and Fe dissolution: oxygen reduction at anodes and cathodes, and hydrogen evolution at cathodes, resulting in net (i.e. oxidation plus reduction) currents at both anodes and cathodes. Investigation results illustrate the feasibility of ferrous and aluminium ion electrochemical treatment as being a successful method of water treatment. Better results were achieved under conditions of relatively high raw water alkalinity, relatively low raw water turbidity, and when high mixing energy conditions were available.

The main purpose of this study is to determine the difference in water quality of the rivers between the Katse and Vaal Dams (Wilge River and Vaal Dam reservoir sub-catchments) after the construction of the Lesotho Highlands Water Project. These rivers include the Ash, Liebenbergsvlei and Wilge Rivers. The temporal changes in water constituents, namely: electrical conductivity, chemical oxygen demand, pH, turbidity, ammonia, calcium, manganese and chlorophyll a, at selected water sampling points were analysed to clarify if Katse Dam water has had any impact on the water quality of the Ash, Liebenbergsvlei and Wilge Rivers and the Vaal Dam. The water quality was studied over an eleven-year period from November 1994 until December 2005. This includes a five-year period prior to, and a six-year period following the completion of the Katse Dam. The Ash, Liebenbergsvlei and Wilge Rivers fall within the Wilge sub-catchment, and the Vaal Dam falls within the Vaal Dam reservoir sub-catchment. Both the aforementioned sub-catchments form part of the Vaal River catchment. Physical, chemical and microbiological sampling results were obtained from Rand Water. The results were compared with the in-stream water quality guidelines as set by the Vaal Barrage Catchment Executive Committee. The results of the selected constituents were depicted visually in the form of graphs. Trends in the constituents over the period were then determined. The graphs were divided into two sections namely, pre-Katse Dam (before 1999) and post-Katse Dam (1999 to 2005). Differences in water quality before and after the construction of the Katse Dam were determined from sampling and chemical analysis at six locations, and hence evaluations were made whether the release of Katse Dam water has had a significant effect on the water quality results in the Vaal River System. The water quality results with respect to the different water constituents illustrated a distinct change in water quality over the period. Northwards, towards the Vaal Dam, the difference in water quality became less apparent. Sampling points throughout the study area experienced decreases in: electrical conductivity, chemical oxygen demand, turbidity, ammonia, and manganese. Hence, the release of Katse Dam water into the Vaal River system has had a ii positive influence on the water quality and thus changed the riverine environments in the Vaal River system. The high quality water from the Katse Dam that enters the Vaal River system thus initially increases the quality of the water in the recipient system with a lesser effect downstream. The result is an improvement of water quality in the upper reaches of the Vaal River system and no significant influence on the Vaal Dam itself. However, the change in water quality may have a detrimental effect on the river environment as a result of the increased volume of water entering the system and the resultant soil erosion, which serves for further studies. Consequently, the advantageous high quality water from the Lesotho Highlands is not being optimally utilised, hence the proposed recommendation by Rand Water to alternatively transfer Katse Dam water via a gravity-fed pipeline to the Vaal Dam thereby receiving the full benefit of high quality water, leaving river environments unaltered and possibly lowering purification costs. / Prof. J. T. Harmse Prof. H. J. Annegarn