This work is focused on the study of physiology and proteome of the the mite Varroa destructor and on comparison with the honeybee Apis mellifera. Varroa is currently a major problem for beekeeping, because infects most of the colonies. The control of the mite can not be done without residues both in the hive and for example in the honey or other resources used by the man. Another problem can also be the simultaneously discussed issue of the connection with the Colony Collapse Disorder. The internal anatomy of V. destructor was studied by using paraffin histology. On histological sections stained using hematoxylin and PAS was possible to observe the mite digestive system, but also egg, ovaries or brain. The primary aim of this study was to identify the proteins of mite V. destructor and bee A. mellifera as a host of this parasite. For the electrophoretic separation was used two- dimensional gel electrophoresis, where the second dimension was carried out using 12% and 15% SDS-PAGE. The most abundant spots were selected for analysis using MALDI TOF/TOF mass spectrometry. The most abundant protein identified in samples of V. destructor was hexamerin, arginine kinase or hemelipoglycoprotein precursor. Hexamerins were also identified as the major proteins in the pupae samples. On the contrary the main...

Arsenophonus is vertically and horizontally transmitted parasitic bacteria and strengthens its transfer through phenomenon called son killing. Arsenophonus has been detected in the arthropod hosts, insects, ticks and the garden spider Araneus diadematus. The aim of this study is detection parasitic bacteria Arsenophonus in honeybees and its parasite Varroa destructor. We find out that bacteria Arsenophonus is present in both hosts and it is identified as Arsenophonus nasoniae. Detection of bacteria Arsenophonus is discovered for the first time in Varroa destructor mites. We proved that the frequency of bacteria Arsenophonus in mites Varroa destructor is significantly higher than in honeybees. This study shows that the mite Varroa destructor could act as a vector for transmission parasitic bacteria Arsenophonus among honeybees. Results of this study could lead to the future application of Arsenophonus as a biological control for the mite Varroa destructor.