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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Inmunodiagnóstico de neoplasias vesicales en bovinos con hematuria vesical enzoótica bovina utilizando la anti-citoqueratina y anti-vimentina

Montes Flores, Karim Mirella January 2007 (has links)
En el presente estudio se procesaron 14 muestras embebidas en parafina de un total de 28 con procesos neoplásicos vesicales de bovinos con hematuria vesical enzoótica bovina (HVEB). De las 14 muestras: 01/14 ha sido diagnosticada como Papiloma, 04/14 como Carcinomas y 09/14 presentan en el mismo tejido vesical Carcinomas y Hemangiosarcomas, además de describirse en 02 muestras la presencia de “Nidos Angioblásticos”, que aparentemente son células endoteliales agrupadas con características de malignidad y se encontraron en el mismo tejido de dos Hemangiosarcomas. Por consiguiente, se tienen 5 muestras de neoplasias epiteliales, 3 de ellas bien diferenciadas y 2 poco diferenciadas; 9 neoplasias mixtas (epitelial y mesenquimal); de las epiteliales, 4 fueron bien diferenciadas y 5 poco diferenciadas; y de las mesenquimales, 6 fueron bien diferenciadas y 3 poco diferenciadas. Para el desarrollo del presente trabajo se utilizó el Método Inmunohistoquímico de la Estreptavidina-Biotina Marcada con los anticuerpos comerciales pre-diluidos AntiCitoqueratina, para el diagnóstico de las neoplasias epiteliales y mixtas (epitelialmesenquimal) del lado epitelial; y la Anti-Vimentina para el diagnóstico de las neoplasias mixtas (epitelial-mesenquimal) del lado mesenquimal; y así corroborar el origen blastodérmico de las células neoplásicas y de los nidos angioblásticos mencionados. Los citoplasmas de las células neoplásicas de origen epitelial se tiñeron de color marrón con el anticuerpo Anti-Citoqueratina; asimismo, los citoplasmas de las células neoplásicas de origen mesenquimal se tiñeron de igual color con el anticuerpo Anti-Vimentina. Todas las células neoplásicas (incluyendo las poco diferenciadas) resultaron positivas al anticuerpo del cual se sospechaba su origen, corroborando al 100% su diagnóstico histopatológico. Las células de los “Nidos Angioblásticos” reaccionaron positivamente con el anticuerpo Anti-Vimentina confirmando su origen mesenquimal; por lo tanto, también se demuestra que son células endoteliales malignas en proliferación debido a sus características histopatológicas como estados primigenios al desarrollo de un hemangiosarcoma. Palabras Claves: Bovinos, Vejiga, Inmunohistoquímica, HVEB, neoplasias, anticitoqueratina, anti-vimentina. / -- In the present study, 14 paraffin-embedded samples were processed, with a total of 28 samples with vesical neoplastic processes of cattle with bovine enzootic vesical hematuria (BEVH). Of the 14 samples: 01/14 has been diagnosed as papilloma, 04/14 as carcinomas, and 09/14 presented in the same bladder tissue, carcinoma and haemangiosarcoma, also described in 02 samples the presence of so-called “angioblastic nests”, that are apparently endothelial cells grouped with features of malignancy and were found in the same tissue of two haemangiosarcomas. Therefore, there were 5 samples of epithelial neoplasms, including 3 well-differentiated and 2 poorly differentiated, 9 mixed tumors (epithelial and mesenchymal); of epithelial neoplasms, 4 were well differentiated and 5 poorly differentiated, and of mesenchymal neoplasms, 6 were well differentiated and 3 poorly differentiated. For the development of this work, there were used the immunohistochemical method of streptavidin-biotin marked with the pre-diluted commercial antibodies anti-cytokeratin, for the diagnosis of epithelial and mixed neoplasms (epithelial-mesenchymal) to the epithelial side, and the anti-vimentin in the diagnosis of mixed neoplasms (epithelial-mesenchymal) to the mesenchymal side, and thus corroborate the blastoderm origin of neoplastic cells and nests angioblastic mentioned. The cytoplasm of the neoplastic cells of epithelial origin were stained brown with anti-cytokeratin antibody; also, the cytoplasms of the neoplastic cells of mesenchymal origin were stained the same color with anti-vimentin antibody. All neoplastic cells (including poorly differentiated) were positive to antibody which was suspected source, corroborating to 100% the histopathological diagnosis of neoplasms diagnosed. The cells of the "angioblastic nests" reacted positively with antivimentin antibody confirming their mesenchymal origin and therefore also shown to be malignant endothelial cells proliferating due to its histopathologic features as primordial state to development of a haemangiosarcoma. Keywords: bovines, vesical bladder, immunohistochemical, BEVH, neoplasms, anti-cytokeratin, anti-vimentin. / Tesis

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