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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

The role of interleukin-8 as a neutrophil chemoattractant In bovine <i>pneumonic pasteurellosis</i>

Caswell, Jeffrey Llewellyn 01 January 1999 (has links)
The role of interleukin-8 (IL-8) as a neutrophil chemoattractant in bovine pneumonic pasteurellosis was investigated by characterizing: (1) the expression of IL-8 in the lesions of pneumonic pasteurellosis, (2) the ' in vitro' and 'in vivo' effects of recombinant bovine IL-8 on neutrophil chemotaxis, and (3) the importance of IL-8 as a neutrophil chemoattractant in this disease. The expression of IL-8 in bovine pneumonic pasteurellosis was assessed by Northern analysis, 'in situ' hybridization and enzyme-linked immunosorbent assay. The expression of IL-8 mRNA was elevated dramatically in lesional lung compared to non-lesional lung, viral pneumonia, and normal lung. 'In situ' hybridization revealed intense IL-8 mRNA expression in alveolar macrophages and neutrophils, and milder expression in several other cell types. Bronchoalveolar lavage (BAL) fluid from lesional lung contained 16.06 ± 4.00 ng/ml IL-8, but lower levels were present in non-lesional lung, viral pneumonia, and normal lung (mean ± SEM). Recombinant bovine IL-8 (rbIL-8) was produced using a pGEX expression system. The rbIL-8 induced <i>in vitro</i> chemotaxis and shape change of bovine neutrophils at doses as low as 6.3 ng/ml. After injection of 1.0 ng to 3.3 <math> <f> <g>m</g></f> </math>g of rbIL-8 into bovine skin, neutrophils marginated in vessels within 15 minutes and infiltrated the dermis by 1 hour after injection. These results demonstrate that bovine IL-8 is a neutrophil chemoattractant 'in vitro ' and 'in vivo', at levels equivalent to those present in pneumonic pasteurellosis. An ovine model of pneumonic pasteurellosis was developed to ascertain the effects of <i> in vivo</i> neutralization of IL-8 on the development of disease. At 0, 3, 8 and 20 hours after bacterial infection (p.i.), BAL fluid samples contained 6.5 ± 2.4, 110 ± 48, 554 ± 118, and 507 ± 70 ng/ml IL-8, respectively. Neutrophil infiltration was mild at 3 hours p.i. but progressively more intense at 8 and 20 hours p.i. Lambs were treated intravenously with up to 80 mg of an IL-8-neutralizing antibody prior to bacterial challenge. The antibody treatment failed to completely neutralize IL-8 activity, probably because the levels of IL-8 in the lung were unexpectedly high. The contribution of IL-8 to the neutrophil chemotactic activity of BAL fluid from cattle and lambs with pneumonic pasteurellosis was assessed. Pre-treatment of pneumonic BAL fluid samples with a neutralizing antibody to IL-8, compared to pretreatment with a control antibody, reduced the 'in vitro' neutrophil chemotactic activity significantly, by 15-63% in 9/10 samples. An extract of pneumonic bovine lung induced vigorous neutrophil infiltration following injection into bovine skin, and depletion of IL-8 from the extract reduced this neutrophil influx by 60%. These data indicate that IL-8 is an important neutrophil chemoattractant in both early and well-developed lesions of pneumonic pasteurellosis, but that mediators with actions redundant to those of IL-8 must also be present at these times.

Comparing diagnostic techniques for detecting intestinal parasites and phylogenetic analysis of Babesia in captive baboons

Gray, Kristene Marie. January 2009 (has links) (PDF)
Thesis (M.S.)--Oklahoma State University, 2009. / Vita. Includes bibliographical references.

Role of MMP2, MMP3 and MMP9 in the development of breast cancer brain and lung metastasis in a syngeneic rat model

Mendes, Odete Rodrigues, 1975- January 1900 (has links)
Thesis (Ph. D.)--Texas A&M University, 2005. / "Major Subject: Veterinary Pathology" Title from author supplied metadata (automated record created on Nov. , 09:45:50.) Vita. Abstract. Includes bibliographical references.

Tumor angiogenesis, O₂ saturation, glucose and amino acid metabolisms study using functional imaging

Xie, Xueyi. January 1900 (has links)
"Major Subject: Veterinary Pathology" Title from author supplied metadata (automated record created 2010-03-12 12:08:51). Includes bibliographical references.

The role of neutrophil recruitment in the pathogenesis of salmonella enterica serotype typhimurium-induced enteritis in calves

Nunes, Jairo Santos. January 1900 (has links)
"Major Subject: Veterinary Pathology" Title from author supplied metadata (automated record created 2010-03-12 12:08:51). Includes bibliographical references.

Molecular epidemiology of Pasteurella multocida respiratory disease in beef cattle

Taylor, Jared D. January 2008 (has links) (PDF)
Thesis (Ph. D.)--Oklahoma State University, 2008. / Vita. Includes bibliographical references.

The significance of bone marrow examination in certain diseases of the bovine and canine

Griffing, William James January 2011 (has links)
Digitized by Kansas State University Libraries

Correlation of clinical and pathological findings of avian encephalomyelitis virus in young chicks

Ostendorf, Joe H January 2011 (has links)
Digitized by Kansas State University Libraries

Molecular and immunological analysis of EHV-1 and -4 infections

Cox, Peter John January 1999 (has links)
No description available.

Early Tissue Migration of and Host Response to Brugia Pahangi in Gerbils

Porthouse, Kristina Houpe 05 November 2004 (has links)
The host-parasite interaction during early filarial nematode migration is poorly understood. The objective of this study was to develop a model of early cutaneous filarid migration using Brugia pahangi in the jird (gerbil) host and measure the histologic and cytokine responses during this period. Male gerbils were intradermally inoculated in the left hindlimb with 100 B. pahangi L3 then necropsied at 3 hours, 24 hours, 3 days, 7 days, and 28 days post-infection. Larvae were recovered and tissues collected for histology and cytokine measurement. At 3 hours, most larvae (96.3%) were recovered from tissues associated with the infection site. Migration away from the infection site occurred within 24 hours. By 7 days, larvae were dispersed throughout the lymphatic system, including the spermatic cord lymphatics. Larvae were identified on histologic exam at all time points and were located in the dermis, muscle, lymphatic vessels, and lymph nodes. Predominantly neutrophilic inflammation was frequently present around larvae in the dermis and muscle at 3 and 24 hours. Levels of the cytokines IL-6, TNF, IFN-gamma, and IL-4 were measured in the spleen and popliteal and renal lymph nodes. IL-6 and TNF both showed a peak at 3 hours followed by consistent decline in all tissues. No clear increase in expression was appreciated for IFN-gamma. IL-4 remained low through 7 days and rose by 28 days in all tissues. These results indicate the ability of filarid L3 to rapidly migrate through host tissue and they support intradermal gerbil infection as a model for early filariasis. Cytokine analysis and histology indicated an acute host inflammatory response following initial infection, with Th2 polarization occurring later in the course of infection.

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