Typescript (photcopy) / x, 194, xxxvi leaves, [5] leaves of plates : ill. ; 31 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1975

The Type 2 Secretion System (T2SS) is responsible for the transport of toxins and enzymes across the outer membrane of many Gram-negative bacteria. A crucial component of the T2SS is a large pore, composed of a multimer of EpsD, named the secretin. This pore inserts in the outer membrane with the assistance of a pilotin (EpsS) or assembly factors (EpsAB), both of which are present within the genome of Vibrio cholerae. The goal of this study was to determine whether or not both assembly mechanisms operate on the same secretin assembly in V. cholerae. Protease deficient mutants generated from an insertion transposon library in V. cholerae epsAB were analyzed. The transposon was found to disrupt the operon encoding VC1702 and epsS. Mutant strains of V. cholerae were constructed or obtained that are deficient in epsA, epsB, epsC, and epsS. Double mutants were constructed that were deficient in epsA and epsS or epsB and epsS. These mutants were tested for assembly of the secretin and secretion of lipase, protease, and cholera toxin. The epsA and epsB mutants have slightly reduced levels of secretion and secretin assembly, while the levels in the pilotin mutant are drastically reduced. The double mutants had little to no assembly, and secretion was reduced to the levels of the control mutant epsC. In an attempt to restore function epsAB was over-expressed in all strains. It successfully complemented the epsA and epsB mutants, and restored levels of secretion to epsS levels in the double mutant, epsAS. In a similar manner to epsAB complementation, epsS was over-expressed. It was found to require the preceding gene VC1702 to complement. The operon, encoding both epsS and VC1702, could complement both epsA and epsS mutations and over-expression increased secretin assembly and secretion to levels greater than wild-type levels. Lastly, a phylogenomic analysis demonstrates that the EpsAB protein complex is found in most orders of the gamma proteobacteria and is ancestral. The pilotins appear to be a late acquisition as they are only found in the family Enterobacteriales.

Bibliography : leaves 147-185. / xi, 185, [6] leaves [16] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1990

Typescript (photocopy) / 132 leaves, [2] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1982

Thesis (Ph.D.) -- University of Adelaide, Dept. of Microbiology, 1975.

Thesis (Ph.D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1982. / Typescript (photocopy).

Thesis (Ph.D.) -- University of Adelaide, Dept. of Microbiology and Immunology, 1975. / Typescript (photcopy).

Thesis (M.Sc. (Tropical Medicine))--Mahidol University, 1978.

Thesis (M.S.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 42-47). Also available on the World Wide Web.

SILVA, G. C. Bioatividade de extratos de algas frente à bactérias com fatores de virulência, resistentes a antimicrobianos. 2012. 61 f. Dissertação (mestrado em Ciências Marinhas Tropicais) - Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, 2012. / Submitted by Nadsa Cid (nadsa@ufc.br) on 2015-04-20T19:31:37Z No. of bitstreams: 1 2012_dis_gcsilva.pdf: 1478354 bytes, checksum: 59498a440456387052763c8870f84f49 (MD5) / Approved for entry into archive by Nadsa Cid(nadsa@ufc.br) on 2015-04-20T19:31:51Z (GMT) No. of bitstreams: 1 2012_dis_gcsilva.pdf: 1478354 bytes, checksum: 59498a440456387052763c8870f84f49 (MD5) / Made available in DSpace on 2015-04-20T19:31:51Z (GMT). No. of bitstreams: 1 2012_dis_gcsilva.pdf: 1478354 bytes, checksum: 59498a440456387052763c8870f84f49 (MD5) Previous issue date: 2012 / Infection of aquatic organisms by Vibrios can lead to serious economic losses for farmers and expose consumers to serious health risks. Some Vibrio species carry factors of virulence making them potentially pathogenic to humans. The consequent widepread use of antibiotics by farmers has led to the selection of resistant strains in the environment. Due to the increasing incidence of bacteria resistant to antibiotics, much effort is currently invested in identifying natural products with bioactive potential. Macroalgae have been studied for their ample spectrum of biological, antioxidant, antifungal and agglutinating activities. The purpose of this study was to evaluate the antibacterial activity of hexane, methanol, acetone and ethanol-based extracts of Chlorophyta, Phaeophyta and Rhodophyta macroalgae against virulent víbrio strains resistant to antibiotics. The microorganisms used in the study included víbrio strains isolated from wild oysters purchased at a fish market, strains from shrimp hemolymph and the standard strains ATCC and IOC. The strains were challenged with extracts using the disk diffusion method. Ethanol was the most efficient solvent. Padina gymnospora inhibited the largest number of Vibrio species (57%) and produced the largest inhibition halos, followed by Ulva fasciata (40%) and Hypnea musciformes (30%). The smallest minimum inhibitory concentration was observed for ethanolic extracts of Padina gymnospora (512 μg/mL against Vibrio alginolyticus). The results suggest extracts of marine macroalgae may represent a potential source of natural biologically active products for use in the industry. / Bactérias do gênero Vibrio acometem a saúde de organismos aquáticos causando sérios prejuízos econômicos a aquicultores e problemas na saúde dos consumidores. Determinadas espécies são consideradas potencialmente patogênicas por possuírem fatores de virulência. Em virtude dos problemas causados por vibrioses, o uso de antimicrobianos em cultivo aquático tornou-se constante, e determinante na seleção de cepas resistentes. Com o aparecimento cada vez maior de bactérias resistentes a antimicrobianos, surgiu o interesse pela busca de produtos naturais com potencial bioativo. As algas tornaram-se alvo de pesquisas por serem responsáveis por um amplo espectro de atividades biológicas, antioxidantes, antifúngicos, aglutinantes, além de antibacterianos. Deste modo, este trabalho objetivou investigar a atividade antibacteriana de extratos hexânicos, metanólicos, acetônicos e etanólicos de macroalgas das classes Chlorophyta, Phaeophyta e Rhodophyta frente a víbrios com fatores de virulência, resistentes a antimicrobianos. Esses víbrios foram isolados de ostras selvagens comercializadas em uma feira de pescado, e de hemolinfa de camarão, além de cepas padrões ATCC e IOC e foram testados através de antibiograma pelo método de difusão em disco. Concluiu-se que: o álcool etílico foi o solvente mais eficaz na extração do composto ativo capaz de inibir o crescimento bacteriano; a Padina gymnospora foi a macroalga que apresentou a maior capacidade inibitória das espécies de Vibrio (57%) e as maiores zonas de inibição, seguida pela Ulva fasciata (40%) e por último a Hypnea musciformes (30%); O menor valor de CIM encontrado foi com o extrato etanólico da Padina gymnospora com 512 μg/ mL contra V. alginolyticus.Os resultados apresentados neste trabalho sugerem que os extratos de macroalgas marinhas poderiam servir como uma fonte potencial de produtos naturais biologicamente ativos para aplicação na indústria.