Includes bibliographical references. The aim of this work is the further characterisation of the citrus exocortis viroid (CEV) replication complex in a tomato host system. The study employed three approaches. i. To raise polyclonal antiserum against a putative catalytic component of the CEV replication complex, the second largest subunit of tomato RNA polymerase II--ii. To develop a cell-free system for CEV RNA synthesis--iii. To use the polyclonal antiserum to the second largest subunit of RNA polymerase II, and a commercially available monoclonal antibody to the largest subunit, for antibody inhibition and immunoprecipitation experiments using the cell-free system.

Includes bibliographical references. / vi, 86, [56] leaves, [10] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The aim of this work is the further characterisation of the citrus exocortis viroid (CEV) replication complex in a tomato host system. The study employed three approaches. i. To raise polyclonal antiserum against a putative catalytic component of the CEV replication complex, the second largest subunit of tomato RNA polymerase II--ii. To develop a cell-free system for CEV RNA synthesis--iii. To use the polyclonal antiserum to the second largest subunit of RNA polymerase II, and a commercially available monoclonal antibody to the largest subunit, for antibody inhibition and immunoprecipitation experiments using the cell-free system. / Thesis (Ph.D.)--University of Adelaide, Dept. of Plant Science, 1997

Bibliography : leaves 92-99. / v, 99, [37] leaves, [14] leaves of plates : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Biochemistry, 1992