No description available.

WRINKLED 1(WRI1), a member of AP2/EREBP class of transcription factors regulates carbon allocation between glycolytic and fatty acid biosynthetic pathway. Additionally, among the four WRI1 paralogs in Arabidopsis, WRI3 and 4 but not WRI2, are also able to increase fatty acid content in different tissues. While the role of WRI1 is well established in seeds, the potential or WRI1 or its paralogs as master regulators in oilrich nonseed tissues is poorly understood. Recent transcriptome studies of avocado (Persea americana) mesocarp revealed that the ortholog of WRI2, along with WRI1 and WRI3 was highly expressed during oil accumulation.Through transient expression assays, wefurther demonstrated thatbothPaWRI1 andPaWRI2 can accumulate oil in tobacco leaves. We conducted a comprehensive and comparative in silico analysis of WRI paralogs from a dicot, monocot and a basal angiosperm to identify distinct features associated with function. These data provide insights into the possible evolutionary changes in WRI1 homologs and allow for identification of new targets to enhance oil biosynthesis in diverse tissues.

Thin film heaters, corrosion-resistance electrode, thin film inductors / Stretchable electronics are soft and light weight. Compared with conventional wafer-based electronics, which are rigid and planar, stretchable electronics can conform to curved surfaces and movable parts. The unique properties of stretchable electronics enable their integration with the human body, and open the door for ever more compelling applications, such as advanced surgical tools, wearable monitoring electronics, implantable prosthesis, and many others. However, the development of stretchable electronics is still at an early stage since their mechanical robustness and electrical performance are still far from satisfying. In this work, I have developed a method to fabricate thin film stretchable devices by solvent-assisted transfer of wrinkled thin films from rigid polystyrene (PS) substrates to elastomeric polydimethylsiloxane (PDMS) substrates. Using this approach, structured thin films containing multiple materials and hybrid structures could be lifted off simultaneously, facilitating the fabrication of stretchable thin film devices. With this approach, I have built corrosion-resistant stretchable electrodes, stretchable thin film heaters, and stretchable thin film inductors. These applications demonstrate the simplicity and effectiveness of this stretchable electronics fabrication strategy. Finally, I made the first step towards fabricating dye-sensitized solar cells (DSSCs) with room temperature processes, including the preparation of mesoporous TiO2 layers through mechanical compression and the integration of an interdigitated electrode that was fabricated solely by bench-top patterning, alignment, and sputtering deposition. These steps lay the foundation for the future development of stretchable DSSC. I anticipate that the fabricated stretchable thin films electronic components will contribute to the advancement of wearable and implantable electronics. / Thesis / Doctor of Philosophy (PhD) / Electronics that can be deformed and conform to the irregular surfaces are attractive because they can be better integrated with the human body. For example, they could improve disease diagnostics and therapeutic treatments by providing wearable continuous monitoring devices and more advanced surgical tools. In this work, I created wrinkled thin films that could be affixed onto an elastic substrate and stretched. The principle of operation of these wrinkled devices mimics the way that the wrinkled skin on our knuckles and elbows allows us to bend our fingers and elbows. This approach makes wrinkled thin films stretchable and could lead to robust electronic devices. I have showcased this approach building a corrosion-resistant stretchable electrode, thin films heaters that can closely conform to joints, and a spiral-shaped inductor that could be used to wirelessly transfer data or power wearable devices. I believe that this work will contribute to the development of electronics that can be worn or implanted in the human body.

This work identifies transcription factors (TF) controlling triacylglycerol (TAG) synthesis and accumulation in plant tissues. TAG plays vital role in plants and are used by humans. Most plants accumulate oil in the seed, but some species accumulate oil in other tissues. The Wrinkled1 (WRI1) TF has been shown to regulate oil accumulation in multiple species and tissues. Here, four WRI homologues in avocado were identified, their phylogeny was examined and three of them were cloned into expression vectors for further characterization. However, WRI1 likely does not act alone in regulation of TAG accumulation in plants. Additional candidate TFs were identified by using transcriptome data from a variety of species, and cloned into expression vectors. Future studies will be able to use this information to better understand regulation of TAG accumulation, which will allow increased oil accumulation in plants for various human uses.

x, 164 p. : ill. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. / We study the topology of the space of metrics of positive scalar curvature on a compact manifold. The main tool we use for constructing such metrics is the surgery technique of Gromov and Lawson. We extend this technique to construct families of positive scalar curvature cobordisms and concordances which are parametrised by Morse functions and later, by generalised Morse functions. We then use these results to study concordances of positive scalar curvature metrics on simply connected manifolds of dimension at least five. In particular, we describe a subspace of the space of positive scalar curvature concordances, parametrised by generalised Morse functions. We call such concordances Gromov-Lawson concordances. One of the main results is that positive scalar curvature metrics which are Gromov-Lawson concordant are in fact isotopic. This work relies heavily on contemporary Riemannian geometry as well as on differential topology, in particular pseudo-isotopy theory. We make substantial use of the work of Eliashberg and Mishachev on wrinkled maps and of results by Hatcher and Igusa on the space of generalised Morse functions. / Committee in charge: Boris Botvinnik, Chairperson, Mathematics; James Isenberg, Member, Mathematics; Hal Sadofsky, Member, Mathematics; Christopher Phillips, Member, Mathematics; Michael Kellman, Outside Member, Chemistry

No description available.

Triacylglycerol (TAG) is a class of lipid molecules composed of three fatty acyl chains esterified to a glycerol backbone. In plants, TAG is synthesized in various tissues and serves as a carbon and energy source. Oil biosynthesis is well understood in oilseeds however how plants store oil in non-seed tissue is yet to be determined. In Avocado (Persea americana), a basal angiosperm, TAG is exclusively accumulated in mesocarp tissue and therefore is emerging as a model system to uncover underlying mechanisms of TAG biosynthesis in tissues other than seed. The mesocarp of Avocado fruit contains ~60-70% of oil by dry weight. Recent transcriptome studies revealed that the TAG biosynthesis is transcriptionally regulated in non-seed tissues. In seed tissues, TAG biosynthesis is regulated by many seed maturation factors directly or indirectly through downstream transcription factor WRINKLED1 (WRI1). Transcriptome studies revealed that in addition to ortholog of WRI1, orthologs for WRI2 and WRI3 were also highly expressed in avocado mesocarp during the period of oil accumulation. Based on the transcriptome data, I hypothesize that putative WRI genes (WRI1, 2, 3) of avocado enhance oil content in nonseed tissues. Currently, cloning of Putative PaWRI 1, 2 and 3 genes into a binary vector, followed by agrobacterium-mediated transformation to generate transient and stable transient lines, is underway. Full-length cDNA for PaWRI genes (1 & 2) were amplified and cloned into pK34 entry vector followed by sequence confirmation. PaWRI genes (1 & 2) were subcloned into pB110 destination vector and will be transformed into agrobacterium for their integration into the plants. Cloning of WRI3 is still ongoing. Transient expression of putative PaWRI 1, 2 and 3 genes, will be validated using tobacco leaf assay, are expected to enhance oil accumulation in leaf tissues. Agrobacterium bearing PaWRI genes and a viral silencing protein (p19) will be co-infiltrated on to the underside of Nicotiana benthamiana leaves. Infiltrated plants will be placed in growth room with 16:8 light/dark cycle. Four days post infiltration, infected leaf areas will be harvested and TAG content and composition will be determined by gas chromatography coupled with flame ionization detector. Functional validation of these orthologs is expected to reveal the preferred WRI isoform that likely participates in regulation of oil biosynthesis in avocado mesocarp. Additionally, this work may also elucidate the differences between regulation of TAG accumulation in seed and non-seed tissues and identify new targets to enhance TAG biosynthesis in plants.

Triacylglycerol (TAG) is a class of lipid molecules composed of three fatty acyl chains esterified to a glycerol backbone. In plants, TAG is synthesized in various tissues and serves as a carbon and energy source. Oil biosynthesis is well understood in oilseeds however how plants store oil in non-seed tissue is yet to be determined. In Avocado (Persea americana), a basal angiosperm, TAG is exclusively accumulated in mesocarp tissue and therefore is emerging as a model system to uncover underlying mechanisms of TAG biosynthesis in tissues other than seed. The mesocarp of Avocado fruit contains ~60-70% of oil by dry weight. Recent transcriptome studies revealed that the TAG biosynthesis is transcriptionally regulated in non-seed tissues. In seed tissues, TAG biosynthesis is regulated by many seed maturation factors directly or indirectly through downstream transcription factor WRINKLED1 (WRI1). Transcriptome studies revealed that in addition to ortholog of WRI1, orthologs for WRI2 and WRI3 were also highly expressed in avocado mesocarp during the period of oil accumulation. Currently, cloning of Putative PaWRI 1, 2 and 3 genes into a binary vector, followed by agrobacterium-mediated transformation to generate transient and stable transient lines, is underway. Transient expression of putative PaWRI 1, 2 and 3 genes, using tobacco leaf assay, are expected to enhance oil accumulation in leaf tissues. Stable expression of PaWRI 1, 2, and 3 in Atwri-/- is expected to restore oil accumulation in seeds. TAG content and composition will be determined by gas chromatography coupled with flame ionization detector. Functional validation of these orthologs is expected to reveal the preferred WRI isoform that likely participates in regulation of oil biosynthesis in avocado mesocarp. Additionally, this work may also elucidate the differences between regulation of TAG accumulation in seed and non-seed tissues and identify new targets to enhance TAG biosynthesis in plants.

No description available.

This research program focused on the behaviour of low cycle fatigue (LCF) of wrinkled pipes, and was designed to develop the LCF life prediction models for the wrinkled pipes. It consisted of three phases of work, which are strip tests, full-scale pipe tests, and finite element analysis (FEA). In strip tests, 39 strip specimens were tested by a complete-reversed stroke-controlled method to investigate the effects of bend angle, bend radius, and stroke range on the low-cycle fatigue (LCF) life. Also, the LCF behaviour was explored by viewing the spectra of key variables and their corresponding hysteresis loops. The failure mechanism was discussed by examining the fracture surfaces. Two LCF life prediction models, life-based and deterioration rate-based, were developed and their prediction results were evaluated. In full-scale pipe tests, two specimens were tested according to a complicated loading procedure. The loading was a combination of axial load, bending moment, and internal pressure; and it consisted of monotonic loading stage and cyclic loading stage. Based on those two tests, the global and local behaviour were investigated, the failure mechanism was studied and the application of the developed LCF life prediction models was discussed. In FEA, three numerical models were developed and they were the strip model, the half-pipe model and the full-scale pipe model. In the strip model, the residual stresses and strains were analyzed and discussed. In the half-pipe model, the effects of pipe geometry, internal pressure, and global deformation on the wrinkle geometry were studied and discussed. In the full-scale pipe model, the full-scale pipe tests were simulated and both the global behaviour and local behaviour were discussed. From this research program, some important conclusions were obtained. The wrinkle geometry is found to be greatly related to the pipe geometry, internal pressure, and global deformation. The global deformation has become localized after the wrinkle is fully developed. The opening deformation cycle is more detrimental to wrinkled pipes than the closing deformation cycle. The test results also show that the seam weld governs the failure of wrinkled pipes if the pipes are subjected to cyclic axial deformation. The LCF life prediction models developed from this research program demonstrate good prediction capacity when they are applied to both strip tests and full-scale pipe tests. / Structural Engineering