INTRODUCTION: The compatibility of EndoSequence BC Sealer (BC Sealer; Brasseler USA, Savannah, GA) for warm vertical compaction has been questioned due to changing physical properties under higher temperature. The aim was to evaluate the effect of heating on the physical properties (flowability and radiopacity) of a new calcium-based root canal sealer (EndoSequence BC Sealer HiFlow [HiFlow]) in comparison with EndoSequence BC Sealer. METHODS: The flow, and radiopacity of the 2 sealers were measured according to ISO 6786/2012 at different temperatures. Forty real printed plastic teeth (incisor) were used to evaluate the flowability of the two Standard EndoSequence BC Sealer and HiFlow EndoSequence BC Sealer into the lateral and apical accessory canals. These evaluation was done with two obturation techniques including single cone and warm vertical condensation. RESULT: The mean flowability was ranging from 22.25 mm to 9.52 mm. The results indicate that there is a statistical difference between the flowability of the two calcium silicate based sealers under all three different temperatures (37˚C, 100˚C and 150˚C). Radiopacity was measured at two different temperatures including 21˚C and 100˚C. The mean gray value ranged from 213.55 to 202.25. Results showed that HiFlow is more radiopaque and there is a statistically significant difference at both temperatures. The results of single cone and warm vertical obturation techniques show that there is no significant difference between the flowability of the two calcium silicate based sealers into the lateral and apical accessory canals at 21°C and there is a significant difference at higher temperatures. CONCLUSIONS: HiFlow BC sealer had higher flow and was more radiopaque, especially at high temperatures, which are generated by the commonly used warm vertical compaction technique.