Approximately 10,000 tones of Hg are deposited annually as a result of anthropogenic activities. This increased Hg burden is known to have adverse neurological and reproductive effects on Common loons. A positive correlation between mercury (Hg) and selenium (Se) has been reported to exist in marine mammals and various species of marine and aquatic piscivorous birds. It has been hypothesized that the Hg/Se interactions may involve in the multiple mechanisms of Hg detoxification. This study focused on the suggested Hg/Se complex that forms in association with specific proteins. Specifically, this project focuses on the nature of these interactions in different tissues from wild Common loons (Gavia immer) that have been collected by the Canadian Wildlife Service. The Hg and Se concentrations in the various tissues were quantified using AAS. MALDI-TOF-MS and protein sequencing characterized the nature of the Hg/Se complex binding protein. Among the tissues, the liver had the highest concentrations of Hg and Se followed by kidney; muscle and brain. A strong association between Hg and Se was found in liver, kidney and eggs whereas there was no association in muscle and brain. In contrast brain and muscle had highest percentage of organic Hg suggesting that only inorganic Hg is associated with Se. Two Hg-Se binding protein complexes were found in liver both in the 15,200-15,300 Da range while one such complex in the same weight range was found in kidneys, when sequenced it was found that these proteins were the alpha A chain of Hemoglobin. The protein complex found in eggs was unique and although it was impossible to fully sequence it, it represents an unknown protein. The role of Se in Hg toxicity in eggs warrants further study.

Heavy metals can serve as a significant component of pollution in watersheds. In this study, ICP-MS analysis was used to determine the heavy metal content (Cd, Cr, Cu, Mn, Pb, and U) in water and sediment of three different Costa Rican watersheds: the Tarcoles River, the Terciopelo Creek, and the Cacao Creek. While the metal content of each the Terciopelo Creek and Cacao Creek was expected to be lower than that of the Tarcoles River, the results showed that this held true only for Cr and Pb. Elevated levels of the other four metals (Cd, Cu, Mn, and U) in the Terciopelo and Cacao Creeks are likely due to natural, lithogenic origin instead of anthropogenic input.

Bacteriological data from 1980 to 1991 were reviewed to determine whether coliform bacteria occurred seasonally and spatially within a midwestern city's distribution system. Coliform bacteria are used as microbiological indicator organisms to determine if a public water supply is safe for consumption. The public water_ distribution system examined had at least a twelve year history of the presence of coliform bacteria. Previous investigations have described the occurrence of the coliform bacteria as sporadic because there were no apparent patterns to their presence. An analysis of bacteriological data has not previously been performed to specifically detect seasonal and spatial occurrences of coliform bacteria.This study attempted to determine if seasonal or spatial patterns of coliform occurrences exist within the in the dominant coliform species. Data indicate that the highest percentage of coliform positive samples occurred in the summer, followed by fall, then winter, with spring having the lowest percentage of coliform positive samples. While Enterobacter cloacae was the dominant coliform species during the spring, summer and fall, Klebsiella oxytoca was the dominant coliform during the winter. Coliform occurrence throughout the distribution system was variable among the 43 sample sites. The percentage of positive samples from the various sites ranged from 0% to 10.5%. The five sites with the highest percentage of coliform positive samples were at the extremities of the distribution system. E. cloacae was dominant at 88% of the sites. K. oxytoca was dominant at 9% sites, which typically had a low percentage of coliform positive samples. / Department of Biology

Freshwater ecosystems will likely become sinks for future silver loadings as a result of increased nanosilver (n-Ag) use in industrial and commercial applications. A series of bioassays was performed to assess how n-Ag toxicity may be influenced by abiotic factors associated with natural freshwater ecosystems. Additionally, these bioassays provide insight into how environmentally relevant concentrations of n-Ag may sublethaly affect the freshwater benthic gastropod, Physa acuta, that plays pivotal roles in maintaining the structure and function of freshwater ecosystems. In sediment with no benthic organic carbon (BOC), gastropod vital rates decreased in treatments containing any n-Ag, gastropods in sediment with relatively low BOC appeared to trade off growth for reproduction at high n-Ag treatments, while gastropod vital rates in high BOC sediment remained unaffected at all nanosilver treatments. Sediment type may abate nanosilver toxicity as a result of organic carbon content. Effects of n-Ag on gastropod vital rates were not dependant on pH, suggesting aqueous pH does not directly influence n-Ag toxicity. Nanosilver (0.2 μg/L) stressed gastropods, altering their growth and reproduction tradeoff dynamics. Nanosilver concentrations modeled to exist in natural freshwaters, disrupted gastropod ability to detect and respond to a natural predator, while greater n-Ag concentrations stimulated gastropods to exhibit contaminant avoidance behavior and thereby attempted to flee their habitat. This study provides direction in understanding how adverse n-Ag effects may be influenced by abiotic parameters, while assessing sublethal effects of n-Ag on freshwater gastropods that are likely to occur in natural freshwater ecosystems, given current estimates of environmental n-Ag concentrations. / Department of Biology

The objective of this research, conducted between November 1972 and March 1974, was to determine the effects of pollution on the benthic macroinvertebrates of Big Lick Creek. Big Lick Creek, a tributary of the Mississinewa River, is located in east central Indiana and has a permanent length of about 32.2 km. Sewage and industrial waste from the cities of Dunkirk and Hartford City are discharged into this stream.The composition and distribution of macroinvertebrate populations, collected with artificial substrate samplers, were used to determine the extent of stream pollution. Samplers consisted of cylindrical barbecue baskets (28 x 18 cm) filled with 12 concrete spheres 7.5 cm in diameter. Samplers anchored to the stream bed at five locations bracketed major sources of pollution. Chemical and physical determinations were performed to provide a general background for existing biological conditions.Significant chemical and bacteriological conditions encountered in the stream below the sewage effluents of Dunkirk and Hartford City, Indiana were as follow: (1) dissolved oxygen minima of 2 ppm or less; (2) free carbon dioxide maxima in excess of 60 ppm; (3) increases in nutrients such as the various forms of nitrogen and phosphate; and (4) drastic increases in fecal coliform densities.Twenty-six species of benthic macroinvertebrates were collected during the study with marked reductions in the number of species occurring below the effluents of Dunkirk and Hartford City. The average number of species occurring below these polluting effluents was 10.5 as compared to 22 farther downstream in recovery zones.Limiting and selective effects of organic pollution were indicated by the composition of the benthic community at each station. Tubificids, tolerant of excessive organic pollution and associated low dissolved oxygen levels, constituted more than 99 per cent of the benthic communities sampled below Dunkirk and Hartford City. Limnodrilus hoffmeisteri, L. udekemianus, and Tubifex tubifex were the dominant species. A maximum tubificid density of 13,050/m2 was obtained below Hartford City.Samples taken from a recovery zone 14.8 km downstream from Dunkirk and a corresponding zone 10.1 km below Hartford City showed more diverse fauna. In the recovery zone below Dunkirk, species other than tubificid represented 17 per cent of the total number of invertebrates collected. In the analogous zone below Hartford City, these species constituted 89 per cent of the total taken. Chironomid larvae comprised 13 per cent of the total obtained in the recovery zone downstream from Dunkirk, and 89 per cent in the similar zone below Hartford City. Chironomus was the dominant chironomid genus.All species collected, in numbers sufficient to be considered, were members of an indicator association (Gaufin 1958) characteristic of organically enriched environments. According to the pollution classification system of Goodnight and Whitley (1961), Stations 1 through 4 were heavily polluted and Station 5 was in good condition.

Automatic river quality monitoring (ARQM) is potentially an important tool in water quality management for the National Rivers Authority (NRA) and similar organisations worldwide. The information produced by ARQM systems must be used in the most effective way and fully integrated with the manual monitoring effort. The status and development of ARQM systems in the freshwater and estuarine River Thames catchment are discussed and a practical appraisal of the design, operation and maintenance requirements given. Data capture, verification and presentation methods are developed and the use of ARQM data for real time management and subsequent analysis is advocated. Examples of data from the freshwater ARQM system are given which emphasise the variability of freshwater quality and the need for a comprehensive understanding of the behaviour of rivers before management decisions are made. The use of ARQM data for assessing the compliance of rivers with River Quality Objectives is examined. With respect to the tidal Thames, data processing methods to correct for the tidal movement of the waterbody are developed. ARQM data are used to highlight the principal factors affecting the water quality of the tidal Thames. The importance of the use of ARQM information in the effective management of the tidal Thames is discussed and operational examples demonstrate how it may be utilised as a basis for management decisions. The application of ARQM to the sub-tropical environment of the River Ganges, India, is investigated. An ARQM system has been designed and prototypes are operational. Extensive site surveys were carried out and the water quality status of the Ganges is discussed. Recommendations for the improvement and future development of ARQM systems are made. The use of ARQM information and its potential for improving the management of rivers is discussed.

The basic theme of this study is that determination of the economic value of water resources is a necessary condition for rational decision-making and management of these environmental assets, and their associated public goods, in developing countries. The research particularly evaluates the contingent valuation (CV) method as a technique for evaluating increments and decrements in environmental and natural resource service flows, and estimates households' evaluations for improvements in river water quality and connections to piped water supply for domestic uses. The study objectives were to (a) estimate the economic value of piped water supply and improved water quality in the Nzoia River Basin, Kenya, (b) evaluate the feasibility of using the CV technique to value an environmental amenity and its related quasi-public service in rural settings where respondents have limited education and monetary resources, (c) examine the role of temporal dimensions of bid payments (i e, frequency of payments) in contingent values for environmental commodities, (d) empirically investigate embedding effect bias in contingent valuation of improvements in river water quality improvement in a less developed economy, and (e) evaluate the role of water connection charges in households' willingness to hook onto piped water supply in Webuye Division, Kenya. Empirical analysis and estimates of the non-market value which local people assign to water quality in the Nzoia River and a private household water connection is based on a detailed survey of a representative sample of 311 households in Webuye Division of Bungoma District, Kenya. In an on-site survey carried out in May through September 1995, contingent markets were developed for the two goods, (1) improved river water quality, and, (2) provision of a private connection to water supply. The corresponding willingness to pay (WTP) values are explained using Ordinary Least Square regression models. Whatever the good, the WTP is seen to increase with income. However, the effects of other factors are more specific to the contingent good. In order of strength, the other determinants of WTP "quality" are sex, age, household ranking of status of domestic water source, distance from river to household residence, the other factors affecting WTP "connections" are existing source of water supply, household size, ranking of river water quality, and age of household head. On the whole, residents accepted the exercise of contingent valuation and were willing to pay important amounts (Ksh 459 and Ksh 386 on average per household per year, respectively, for goods 1 and 2). Discussion issues include policy significance of the resulting WTPs in terms of the demand for river pollution control and individual household water connections, the effect of the goods upon the CV evaluation process, the "Third World" impacts of frequency of payments in contingent valuation, including perceived-frequency and income-smoothing routes, the embedding effect in WTP values for water pollution abatement in the Nzoia River basin, the importance of pricing influences, specially payment profiles for initial connection charges, on household decisions to connect to piped water systems, and limitations of the study.

The effect of lake acidification was evaluated, in the field, in terms of its impact on both the structural and functional composition of the macrozoobenthic community. The littoral macrozoobenthic community and water chemistry of 45 Canadian Shield lakes was sampled. The water chemistry variables sampled included pH, total dissolved calcium, conductivity, and dissolved organic acid (measured as colour). Partial canonical correlation analysis and partial regression analysis were used to identify those components of the macrozoobenthic community that most directly reflected pH variability. This was done by first removing from the data that portion of the variability attributable to total dissolved calcium, conductivity, and dissolved organic acids. In addition, the spatial structure in the data was removed by identifying the geographic coordinates of the sampling sites. / In general, the results presented here are not consistent with previous studies where the response of the macrozoobenthic community was related to pH without consideration of confounding covariables. Snails, leeches, mayflies and crayfish have all been cited for their sensitivity to acidification. The present study found these taxa to reflect, not pH variability, but rather attributes of water hardness. Similar results were found for both total zoobenthic biomass and functional feeding group abundance.

White blood cell phagocytosis was investigated and used with avian blood, and assessed as a potential biomarker for mercury immunotoxicity in free ranging birds (common loons). Phagocytosis is an essential immunological function and can be measured using flow cytometry. The assay was assessed with in vitro exposure using whole blood and isolated white blood cells (WBC) from domestic chickens, and with in vivo exposure using whole blood from captive doves and wild loons. McHg at 0.1ppm significantly depressed phagocytic capacity of isolated WBCs without affecting their viability, but did not affect phagocytic activity when added to whole blood up to 50ppm. Also, no significant relationship between blood-Hg level and phagocytic capacity of WBCs was observed in ringed turtle doves fed McHg in their diets, nor in wild common loons having a range of blood-Hg concentrations. The phagocytosis assay is a convenient assay for use in field studies of free-living birds, but is not responsive to McHg exposure in birds, and so is not recommended as a biomarker of immunotoxicity in Hg-exposed loons.

No description available.