Microplastics (MPs) pollution is a global ongoing issue in freshwater ecosystems. The threat of MPs has only recently been recognized and research is needed to better understand how widespread this threat is. Plastic litter is the ultimate source of MPs and we might therefore expect MPs to be a greater threat in areas of high human use. To improve our understanding of how MPs pollution might vary with human land use, we will survey for MPs in freshwater streams. To find the variation of land use this study would be sampling three major types of streams: forested, urban, and agricultural. MPs will be sampled from three streams in each landscape using 1 L grab samples. The freshwater will be filtered and MPs in each landscape will be compared using ANOVA. Each stream site will be sampled five times for a total of forty-five grab samples. The samples will take place in early spring, and sampling after a storm will be avoided to prevent bias data. The results of the project will improve our understanding of where MPs are a potential threat in East Tennessee, as well as our broader understanding of how human activity influences the distribution of this threat.