The purpose of this study was to analyze the biology content, instructional strategies, and assessment methods of 100 biological science websites that were appropriate for Grade 12 educational purposes. For the analysis of each website, an instrument, developed from the National Science Education Standards (NSES) for Grade 12 Life Science coupled with criteria derived from the Web-based Inquiry (WBI) for Learning Science instrument (Bodzin, 2005) and other pertinent published educational literature, was utilized. The analysis focused on elucidating the appropriateness of the biology content, instructional strategies, and assessment tools of selected websites for facilitating the biological science education of Grade 12 students. Frequencies of agreement and disagreement of the content of each selected website with criteria included in the data collection instrument were used for alignment determination of the content of each website with the NSES. Chi-square tests were performed by Microsoft Excel to determine the statistical significance of differences of actual and expected 85% frequencies of alignment of the analyzed website parameters with indicators of alignment to NSES. Chi-square tests indicated that at a 0.05 level of significance there was an overall difference between the actual and expected 85% frequencies of alignment of biology content, instructional strategies and assessment methods with website indicators of alignment with the NSES (p < 0.05). Chi-square tests also indicated that there was a significant difference between the actual and expected frequencies of alignment of analyzed categories (biology content, instructional strategies, and assessment methods) of the sampled websites with website indicators of alignment with the NSES (p < 0.05). / Major findings of this study indicated that 3 out of 4 of the analyzed content attributes, 12 out of 13 of the instructional strategies, and all the assessment methods of the researched biological science websites were less than 85% aligned with the NSES. Only 11 out of 80 (13.75%) of the analyzed websites had collective biology content, instructional strategies, and assessment methods attributes that were 85% or more aligned with the NSES. Appropriately sequenced content that fostered acquisition of fundamental biology knowledge was the only content attribute with significantly more than 85% alignment with the NSES. Provision of illustrative examples to enhance understanding of facts and/or ideas in the context of a conceptual framework was the only instructional strategies attribute that was significantly more than 85% aligned with the NSES. Alignment of website attributes with the NSES has the potential to enhance the educational value of science websites. It is hoped that the findings of this study will motivate science website designers to comply with the NSES. Hope also exists that educators will be motivated to engage in standards-based reform measures for promoting scientific literacy among students. / by Joy E. Stewart. / Vita. / Thesis (Ed.D.)--Florida Atlantic University, 2010. / Includes bibliography. / Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.