The contamination of heavy metals in the environment is a looming concern worldwide. Egeria densa (Planch) (Submerged aquatic plant) from two ponds: Site A with co-ordinates (32º 48’22.04”S; 26°48’58.79” E) and Site B with co-ordinates (32°48’33.25”S; 26°48’33.25”S) in Alice (Eastern Cape) was evaluated for its ability to absorb heavy metals, phytochemical constituents, antimicrobial activity and ultra-structure using standard analytic procedures. Cadmium (Cd), copper (Cu), iron (Fe), lead (Pb), manganese (Mn), and zinc (Zn) were measured in water, sediments and plant. The concentrations of these metal elements were determined with use of Inductively Coupled Plasma- Optical Emission Spectrometry (ICP-OES). In sediments, the heavy metals (mg/kg) decreased in the order of their average concentration as follows: Fe (40.320) > Zn (1.259) > Pb (0.564) > Mn (0.186) > Cu (0.037) in Pond 1 whereas in Pond 2 Fe (61.527) > Cd (0.999) > Mn (0.648) > Pb (0.586) > Zn (0.156) > Cu (0.045). The highest concentration of Fe was detected in both sites and Cu being the least. The concentrations of the metals in the plants sample (from Pond 1) were found in order of Mn > Pb > Cu > Fe whereas cadmium and zinc were not detected, while the concentration in Pond 2 decreases in order of Zn > Mn > Pb > Cd > Fe > Cu. In the water samples, concentrations of heavy metals (mg/L) decreased in the order of their average concentrations as follows: Pb (35.36) > Fe (3.07) > Mn (0.238) > Cu (0.104), both cadmium and zinc were below the limit of detection in Pond 1, whereas in Pond 2 the concentrations decreased as follows: Pb (13.033) >Fe (1.69) > Cu (0.270) > Mn (0.248) > Cd (0.004) and Zinc was not detected. Phytochemical analyses of the plant extracts revealed the presence of phenols, flavonoids, proanthocyanidin, flavonols, saponins, alkaloid and tannins in all the extracts (water, acetone and n-hexane). Both acetone and water extracts, showed high concentration of proanthocyanidin, while tannin was the lowest in acetone extract. Antimicrobial evaluation using, Gram positive (Staphylococcus aureus, Bacillus pumilus, Bacillus cereus, Streptococcus pyogenes, Enterococcus faecalis) and Gram negative (Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris and Serratia marcescens) bacteria showed negative results for all the strain, except Streptococcus pyogenes which was inhibited at MIC of 0.1 mg/ml. Scanning electron microscopy (SEM) of ultra-structure of Egeria densa, showed that certain bacteria attached to the leaf, However more work has to be done on E. densa to verify the mechanism by which it accumulates heavy metals. The study shows that E. densa has a potential of accumulating heavy metals especial Manganese in plant.

Low cover crop biomass production is a major obstacle to the success of conservation agriculture currently promoted as panacea to the inherent problem of soil erosion and loss of soil productivity in the Eastern Cape (EC). Therefore, this study evaluated cover crop management strategies for optimizing biomass production for better soil cover, soil nitrogen (N) and phosphorus (P) fertility, weed control and maize yields. The strategies tested are cover crop bicultures, selection of an adapted lupin cultivar and seeding rate, and the feasibility of rain fed winter cover cropping. The cover crop experiments were carried in rotation with summer maize between the winter of 2009 and summer of 2010/2011. Biculture trial was carried out by seeding oat (Avena sativa) and vetch (Vicia dasycarpa) at three mixture ratios and as sole crops under irrigation. On a separate irrigated trial, two lupin cultivars (Lupinus angustifolius var Mandelup & Qualinock) were seeded to a range of seeding rate, 40 to 220 kg ha-1. To study the feasibility of rain fed winter cover cropping, oat, vetch, rye (Lolium multiflorum), barley (Hordeum vulgare), radish (Raphanus sativa) and triticale (Triticale secale) were relayed into a maize crop in February, March and April of 2010. The irrigated trials were followed with SC701 maize cultivar, whilst the rain fed trial was followed with DKC61-25 maize cultivar. Bicultures gave higher cover crop biomass than sole vetch, increasing with an increase in the oat component of the mixture. Increased N and P uptake was observed with bicultures compared to sole oat, however, the levels were comparable to sole vetch. Sole vetch increased soil inorganic N and P at maize planting, whilst the slow decomposition by sole oat residue resulted in mineral lock up. Bicultured cover crop residues had intermediate decomposition rates and resulted in optimum levels of inorganic N and P for prolonged periods compared to sole crops. Weed suppression by the bicultures was comparable to sole cover crops. Biculturing technology significantly (P<0.05) increased maize grain yield compared to sole oat and the yields were comparable to those from sole vetch. For lupins, 206 kg ha-1 seeding rate gave the optimum biomass yield. Weed dry weights in both cover crop and maize crop decreased with an increase in lupin biomass. Comparable soil total N and inorganic P values at maize planting, were observed from plots planted to 120, 180 and 220 kg ha-1. Maize grain yield increased with an increase in lupin seeding rate. The study on rain fed winter cover cropping had most cover crop species’ biomass decreasing with each delay in planting except for radish, which increased. Vetch produced the highest amounts of biomass from February and March planting whilst radish had the highest biomass in April planting. The two species resulted in the greatest N improvement compared to the other species. Regardless of the grazing, the grass specie residues managed to persist to the next cropping season and the residue remaining were comparable to that of radish and vetch. Late-planted cover crops had the greatest residue remaining than early-planted, as a result, April planted cover crops provided better weed suppression than March and April planted. Vetch provided the highest maize grain yield (4005 kg ha-1) whilst all other species tested had comparable grain yields. The results suggested that bicultures could be grown to give sufficient biomass for both weed suppression and soil fertility improvement. Furthermore, increasing lupin plant densities improve its function as a cover crop with respect to weed suppression, soil fertility improvement and maize yields. The study also showed that for dry land systems, February and March planted vetch and April planted radish can provide the greatest biomass and maize yield improvement.