To ensure effective detection of bio-particles, it is crucial to understand the effects of collection method and archiving conditions on the survivability of bioaerosols, consequently, the survivability of the spore-forming Bacillus globigii (BG) and MG1655 Escherichia coli (E. coli), was determined after collection. The survivability was defined as the culturable fraction of the archived bacteria/culturable fraction of the as-collected bacteria. The bacteria were aerosolized for up to four days at room temperature (RT, 25 degrees C) and at 4 degrees C and collected in a 100 L/min wetted wall cyclone (WWC) and a 12.5 L/min SKC BioSampler. Aqueous solutions of 0.01% Tween-20 and 30% Ethylene Glycol (EG), with or without 0.5% ovalbumin (OA), were used as the collection fluids. Antifoam B (A-F), at a concentration of 0.2% (V:V) was added to the BG samples containing OA. In general, samples archived at 4 degrees C showed higher survivability than at RT. The survivability were more stable in EG than in Tween-20 especially for BG, very likely due to the surfactant effect of the Tween-20, which would remove the spore coat and initiate germination. In the WWC, adding OA significantly increased the survivability of BG in EG and in Tween-20, especially at RT. Similar effect of OA was found for E. coli samples stored in EG, suggesting that OA might be beneficial in maintaining the survivability. Adding A-F increased the survivability of BG in EG. In the SKC, neither the addition of OA nor A-F seems to have a beneficial effect on the survivability of the spores in EG samples. The best collection fluid for maintaining survivability in the WWC is EG+A-F for BG, and EG+OA for E. coli. However, in the SKC, EG is the best for BG collection and Tween-20 for E. coli. Viability transfer ratios, VTR, (cells surviving collection at time zero/viable cells aerosolized) were calculated for both devices. A performance ratio was calculated as the VTR of the WWC/VTR of the SKC. The geometric mean of the performance ratio is 1.51+/-0.83 for BG and 2.60+/-0.16 for E. coli, indicating that viability transfer ratio of the WWC is typically higher than that of the SKC.

In this study, a system for the collection of bioaerosols emitted from the mail sorting process was designed and characterized. Two different wetted-wall cyclones, the JBPDS cyclone and the glass cyclone sampler (GCS), were evaluated as system collection devices. These devices operate at 780 L/min and have a D50 of ~ 1 μm. A trimming impactor with a D50 of 10 μm was used upstream of the collection devices. Using two reference probes, the cyclone liquid outputs were compared with aerosol collected on filters and the output of an Aerosol-to-Hydrosol Transfer Stage (AHTS). The mass emission rate of the postal sorting machine was 3.15 mg/min and found not to vary significantly with flow rates above 700 L/min. On average, greater than 66% of the mass collected had a Da < 10 μm. Using a Coulter Counter, the volume median diameter (volume equivalent) for both device hydrosol outputs was 4.18 μm. For the effluent aerosol, the volume median diameter was 12.5 μm. For a bioaerosol release, this study found that greater than 65% (by volume) of the material released had a Da greater than 7.2 μm. Using filters, it was found that on average, 95% of the bioaerosol particles emitted had a Da less than 10 μm. According to the reference data, the expected number of bioaerosol particles in 1.5 times that collected by the GCS and 5.5 times that collected by the JBPDS cyclone for a one milligram release. The time constant for the system in response to a letter release was found to be 1.3 minutes for the GCS and 1.75 minutes for the JBPDS cyclone. A final note to this study states that the probe dimensions were incorrectly developed, therefore affecting the aspiration efficiency of the probes. In turn, this may have affected the outcome of some of the results. A plot is given at the end of the paper showing to what extent the results may have been affected. It is recommended that further experimental studies be performed to verify the results in this study.