Road networks have significant impacts on ecosystems through deforestation, rapid land conversion and wildlife-vehicle collisions. Road ecology seeks to analyze the spatial and biological patterns of collisions to understand the effect of roadways and best inform transportation planning in mitigating these threats. 215 km of roadway BR-262, that bisects critical habitat of the Brazilian Cerrado and Pantanal in southwest Brazil, was analyzed between April 2011 and June 2012 to assess how biological, physical and landscape characteristics affect clustering of collisions. 518 collisions, representing 40 species, were found to be seasonally clustered between the unimodal rain and flood pulse in a February-March-April window and were spatially clustered over the study area in relation to cash crop and cattle ranching land-use and the rural low-lying Pantanal floodplain. Mitigation seeks to work with local communities and transportation agencies to facilitate fauna safe crossing through the roadway that do not conflict with drivers. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2015. / FAU Electronic Theses and Dissertations Collection