Built-up timber beams consist of individual lumber laminations connected together using mechanical fasteners such as nails, bolts and screws. Lateral torsional buckling (LTB) is an important failure mode that needs to be considered in deep beams with long spans and insufficient lateral supports. Due to the mechanical connectors, built-up beams are expected to have a lower moment capacity compared with solid beams with similar dimensions. The behaviour of built-up beams is greatly affected by the stiffness of the fasteners joining the individual laminations and determining the level of partial composite action attained in the beam. The current research aims to investigate the buckling behaviour of timber built-up beams. This is done by initially investigating the important parameters that play a role in the behaviour through an extensive sensitivity analysis. The focus of the analysis is on the contribution of the connections, since the buckling behaviour of individual solid timber beam element has been relatively well-established. Input parameters for the connection properties are obtained from joint level experimental tests. Finally, recommendations for specific fastener patterns and accompanying reduction factors on the buckling capacity relative to equivalent solid sections are developed and proposed.