Brain damage in the first week of life is behaviorally and anatomically devastating for a rat. I investigated the use of pre- and/or postnatal experience as interventions that might improve the outcomes in rats with postnatal day 4 (P4) frontal cortex lesions. Prenatal maternal tactile stimulation or maternal complex housing facilitated recovery in P4 lesion animals and produced changes in brain organization. Post-lesion tactile stimulation also was found to be beneficial possibly via experience dependent changes in FGF-2 expression. Levels of FGF-2 were increased in both skin and brain after tactile stimulation and correlated with behavioral and anatomical changes. Direct post-lesion administration of FGF-2 had similar effects. These results are the first demonstration that prenatal experience can be prophylactic for postnatal brain injury and that behavioral experience can act on brain organization via enhanced trophic factor expression originating in skin. / xxi, 221 leaves : ill. ; 28 cm.

Basic fibroblast growth factor (FGF-2) is a trophic molecule involved in a number of functions within the central nervous system (CNS), including a prominent role in the regulation of CNS responses to injury. Prior studies suggest that rats recover differently from injury inflicted to different regions and at different ages throughout development, and that FGF-2 might underlie this phenomenon. This thesis examined whether the functional and structural outcome following bilateral injury to the motor cortex inflicted at postnatal day (P10) could be ameliorated by exogenous administration of a growth factor (FGF-2). Four complimentary studies were conducted that each assessed the role of FGF-2 in mediating recovery from bilateral motor cortex injury inflicted at P10. We found that FGF-2 improves physiological, anatomical, and functional outcome from bilateral lesions to motor cortex at P10. / xiii, 171 p. : ill. ; 28 cm.

Basic fibroblast growth factor (bFGF) has been implicated in the brain's trophic response to injury. This thesis examined the effects of endogenous bFGF on brain plasticity and recovery of behavioral function following cortical injury in adult rats. The first experiment investigated the post-lesion time course of the astrocytic expression of bFGF. Subsequent experiments examined the effects of injury-induced bFGF on neuroonal morphology, cortical morphology, and post-lesion behavioral deficits. Following motor cortex injury, endogenous bFGF prevented neuritic degeneration in layer V pyramidal neurons in Zilles' area Fr2 and promoted recovery of function in the Whishaw Reaching Task. Housing rats in an enriched environment prior to cortical injury enhanced the expression of bFGF but did not increase cortical thickness nor reduce post-lesion behavioral deficits (relative to laboratroy-housed rats). Collectively, these experiments indicate that injury-induced bFGF plays a role in potentiating recovery from brain damage. This implies that bFGF may be beneficial as a treatment following brain injury. / x, 123 p. ; 28 cm.

The current studies investigate the behavioral and anatomical changes after lesions at different ages of the cingulate cortex. Rats received lesions of the posterior cingulate cortex (PCing) or the anterior and posterior cingulate cortex (Total) at: postnatal day 4 (P4); day 10 (P10), or in adulthood (P120). Rats were trained in the Morris water maze, the Whishaw reaching task, conditioned taste aversion (CTA), and their activity was monitored over 48 hours. The general finding was a significant behavioral recovery on P10 animals regardless the size of the lesion. This recovery was associated with an increase in dendritic arborization in P10 animals with the PCing removed and a partial regeneration of the midline tissue in the Total P10 animals. These results suggest that damage to the cingulate cortex at P10 is associated with substantial behavioral and anatomical plasticity and that removal of the frontal midline tissue stimulates a regenerative process in more posterior cortex that does not occur otherwise. / ix, 111 leaves : ill. ; 29 cm.

This thesis examined the role of exogenous basic fibroblast growth factor (FGF-2) in stimulating recovery after early cortical injury. Rats with medial prefrontal cortex (MFC), posterior parietal cortex (PPC), or sham lesions at postnatal day 3 (P3) received one of three variations of FGF-2 treatment: postnatal FGF-2 that was either pre-mixed or prepared daily, or prenatal FGF-2, and tested in adulthood. Behavioral tests used were: 1) the Morris Water task and, 2) the Whishaw Tray Reaching task. The level of functional recovery attained was dependent on FGF-2 preparation and the developmental period. MFC lesion rats showed good recovery but there was a differntial effect of pre and postnatal FGF-2 that appeared to be related to task. PPC rats showed greater recovery after postnatal rather than prenatal treatment. Anatomical changes were restricted to groups with relatively good functional recovery. These findings suggest a multifunctional role of FGF-2 in the injured brain. / xvi, 223 leaves : ill. ; 29 cm.

Previous work has shown that removal of the midline frontal cortex at seven to ten days of age is followed by recovery of function correlated with apparent spontaneous generation of new tissue in the lesion cavity. The question asked in the present thesis was whether the removal of the regrown tissue in adulthood would block normal function. Rats that received P10 frontal lesions underwent second lesions at P160, and were compared to rats with only P10 or P160 lesions. Rats with P10 + P160 lesions were severely impaired on a spatial learning task, especially relative to the P10 lesion-only rats. In a second experiment, rats with P10 + P160 lesions were given intra-ventricular infusions of a cocktail of three growth factors. The animals with growth factors showed marked behavioral recovery, although there was no cell regeneration. The results of these experiments suggest that filled-in tissue in neonatally lesioned rats is functional. / viii, 74 leaves : col. ill. ; 28 cm.

The current work explores the behavioural and anatomical consequences of unilateral neonatal bulbectomy (OBX) in male and female rats at postnatal day 1 (PI) and P10. In adulthood the animals underwent a battery of motor and cognitive tests, and diffuse effects of early brain injury on the development of behavior were found. Disturbing olfactory sense input during development affected motor output. Rats normally display an equal distribution of right or left paw preference. In this study, both OBX sexes showed a shifted paw preference to the ipsilesional side, and forelimb deficits were found in a skilled reaching task. Lesion animals also showed enhanced performance on a visually driven spatial cognitive test. Cross-modal compensatory changes may be responsible. Morphological changes within the cerebral cortex are described, including bulbar changes, enlarged but fewer glomeruli, smaller accessory olfactory bulb, decreased downstream connectivity, and a rostral shift of the forebrain toward the olfactory bulb. Changes to the lateral cortex were found in both intact and lesion hemispheres, along with dendritic changes in the forelimb reaching area. Cellular regeneration within the lesion bulb was indicated. Changed shape and relative size increases compared to the intact bulb were found. BrdU labeling showed increased mitotic activity in P10 lesion animals. These findings demonstrate that the impact of olfactory injury during early development goes well beyond odor perception and discrimination, and that olfactory inputs during development significantly contribute to the development of the neocortex. / xix, 195 leaves : ill. (some col.) ; 29 cm.

Thesis (M.Tech.: Chiropractic)-Dept.of Chiropractic, Durban Institute of Technology, 2003 / Medial Tibial Stress Syndrome Type II (MTSS), otherwise known as shin splints, accounts for approximately 13% of injuries in American runners. Van Mechelen (1992) reported that 37-57% of recreational runners experience an injury over the course of a year, from which 54-75% of all injuries are caused by overuse. The American Medical Association defines shin splints as “pain and discomfort in the leg from repetitive activity on hard surfaces, or due to forceful, excessive use of foot flexors. The diagnosis should be limited to musculoskeletal inflammations excluding stress fractures and ischemic disorders.” (Thacker et al., 2002) Treatment protocols vary from biomechanical interventions (orthotics), to non-steroidal anti-inflammatory drugs and modalities such as ultrasound all with varying degrees of success (Noakes, 2001). Apart from therapeutic interventions it is the overriding symptom of pain, which patients are left with (Noakes, 2001). A therapeutic intervention called periosteal pecking has received increased interest with regards to symptomatic treatment of shin splints. Periosteal pecking is a form of *dry needling in which the tip of the needle contacts the periosteum (Raso,1997). The aim of this study is to establish the effect of periosteal pecking in the clinical setting with and against that of an established intervention, namely therapeutic ultrasound.

The aim of this study was to investigate which protocols Occupational Therapists (OT’s) use when rehabilitating clients after flexor tendon repairs, and to investigate the therapist’s knowledge regarding these protocols, to guide therapists and institutions in using effective methods within the South African context. A questionnaire was sent to OT’s in South Africa. Of the 32 responses, 50% had more than 10 years experience and 50%, less experience. 81.2% were private practitioners and 28% worked in government. The trend of protocol use was as follows: 18.8% used a Duran-type passive mobilization protocol, 25% used a Kleinert-type protocol - a passive flexion protocol (but labelled an active mobilization protocol in literature as it allows active extension of the fingers), 28.1% used Early Active Mobilization and 3.1% used an Immobilization-type protocol. 64.5% of the sample used static splints, 9.7% used dynamic splints and 25.8% used a combination. Most (83.3%) continued the splint at 4 weeks but only 26.6% were using the splint at 6 weeks. At week 1, 30% allowed active flexion of the fingers, whereas at week 4 and 5, 60% allowed active flexion. The referring doctor and confidence in one’s own skills were the main factors influencing protocol choice. Resources available influenced the protocol choice, which can be problematic in South Africa. Access to literature was mostly through textbooks (90.6%), although journal articles were accessed (internet - 50%, hard copy - 62.5%). More than half of the sample attended courses regularly. Most therapists were happy with their outcomes, regardless of which protocol used. Therapists need to build their confidence, realising the efficacy of various protocols is similar, according to research. Thus whatever factors influence protocol choice, they will likely not be critical to good outcomes. / Thesis (M.O.T.)-University of KwaZulu-Natal, Durban, 2012.

Introduction: Garment work is repetitive and detailed and requires constant use of the hands. It is no surprise that garment workers are at high risk for developing repetitive strain injuries (RSI’s) (MFL Occupational Health Centre, 1999). Work-related upper limb disorders, popularly known as RSI’s, affect over 370,000 people in Great Britain with 86,000 new cases recorded in 2010. This costs employers almost £300 million in lost working time, sick pay and administration (The Chartered Society of Physiotherapy, 2007). There is however no statistics documented on RSI’s among garment workers found for South Africa. Aim: To determine the prevalence of signs and symptoms of carpal tunnel syndrome and de Quervains tenosynovitis. Method: A study using quantitative data was used. A validated questionnaire consisting of open-ended and closed questions was utilized. Data was collected from two hundred subjects of varying age, gender and ethnic group. Information on signs and symptoms and possible risk factors of RSI’s were obtained. The visual analogue scale was used to assess pain, a goniometer to measure active range of movement, the Phalens test, Reverse Phalens test and Finkelsteins test was used to assess the signs and symptoms of the two occupational repetitive strain disorders. Data analysis: All data was captured and analysed using the Statistical Package for Social Sciences (SPSS version 15). Descriptive statistics such as mean, standard deviation, proportions, median, mode and interquartile range was used to summarize the data. Pearson’s Chi Square tests and Fishers Exact tests were used to test for association between two categorical variables. Independent Samples t-tests were used for the difference in age distribution between participants that presented with carpal tunnel syndrome and de Quervains tenosynovitis and of those who did not present with them. The level of significance was set at 0.05. Bar graphs, tables and pie charts were used to depict the results. Results/Discussion: The results of this study indicated that 59% of participants presented with signs and symptoms of de Quervains tenosynovitis and 63% of participants presented with signs and symptoms of carpal tunnel syndrome. The prevalence of carpal tunnel syndrome and de Quervains tenosynovitis was 42% and 43% respectively among garment workers in the eThekwini district. In addition, 100% of participants stated that they work under the following conditions, applying weight through the arms, repeated movement, work with their arms in unsupported positions, fast hand movements and holding or grasping for more than 2 hours continuously per day. Seventy two and a half percent of participants stated that their work entailed using vibratory tools for prolonged hours. Pearson’s Chi Square tests showed no association of use of vibratory tools to de Quervains tenosynovitis (P=0.666) or to carpal tunnel syndrome. This is inconsistent with the findings of the study completed by Leclerc et al. (1998) who stated that different dimensions of exposure to physical workload are widely recognised as risk factors. These risk factors include rapid hand motions, repetitive bending and twisting of the hands and the wrist, fast work pace, repetitive grasping with the fingers, mechanical stress at the base of the palm and the palm and the use of vibratory tools (Leclerc et al. 1998). Conclusion: This study has identified the prevalence of signs and symptoms of carpal tunnel syndrome and de Quervains tenosynovitis among garment workers. It has also shown that a significant percentage of garment workers presented with symptoms of burning, tingling, itching and numbness in their hands as well as feelings of swollen and ‘useless’ hands. A significant number presented with functional limitations to certain activities of daily living suggestive of the presence of carpal tunnel syndrome. De Quervains tenosynovitis was indicated when a significant number of participants presented with pain, tenderness or swelling over the radial aspect of the wrist as well as functional limitations to certain activities of daily living. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2012.