Objectives: To detect and distinguish oral malignant and non-malignant lesions from clinical photographs using YOLO v8 deep learning algorithm. Methods: This is a diagnostic study conducted using clinical images of oral cavity lesions. The 427 clinical images of the oral cavity were extracted from a publicly available dataset repository specifically Kaggle and Mendeley data repositories. The datasets obtained were then categorized into normal, abnormal (non-malignant), and malignant oral lesions by two independent oral pathologists using Roboflow Annotation Software. The images collected were first set to a resolution of 640 x 640 pixels and then randomly split into 3 sets: training, validation, and testing – 70:20:10, respectively. Finally, the image classification analysis was performed using the YOLO V8 classification algorithm at 20 epochs to classify and distinguish between malignant lesions, non-malignant lesions, and normal tissue. The performance of the algorithm was assessed using the following parameters accuracy, precision, sensitivity, and specificity. Results: After training and validation with 20 epochs, our oral cancer image classification algorithm showed maximum performance at 15 epochs. Based on the generated normalized confusion matrix, the sensitivity of our algorithm in classifying normal images, non-malignant images, and malignant images was 71%, 47%, and 54%, respectively. The specificity of our algorithm in classifying normal images, non-malignant, and malignant images were 86%, 65%, and 72%. The precision of our algorithm in classifying normal images, non-malignant images, and malignant images was 73%, 62%, and 35%, respectively. The overall accuracy of our oral cancer image classification algorithm was 55%. On a test set, our algorithm gave an overall 96% accuracy in detecting malignant lesions. Conclusion: Our object classification algorithm showed a promising application in distinguishing between malignant, non-malignant, and normal tissue. Further studies and continued research will observe increasing emphasis on the use of artificial intelligence to enhance understanding of early detection of oral cancer and pre-cancerous lesions. Keywords: Normal, Non-malignant, Malignant lesions, Image classification, Roboflow annotation software, YOLO v8 object/image classification algorithm. / Oral Biology