Thesis (M.S.)--Northern Michigan University, 2008. / Bibliography: leaves 107-114.

Thesis (Ph. D.)--Auburn University, 2008. / Abstract. Vita. Includes bibliographical references.

Thesis--Københavns universitet. / "Translation by Niels Haislund"--T.p. verso. Includes bibliographical references (p. [128]-132).

Thesis--Københavns universitet. / "Translation by Niels Haislund"--T.p. verso. Includes bibliographical references (p. [128]-132).

Hybridization studies based on the prototrophic selection technique, involving the use of auxotrophic mutants of strains of all accepted species of the genus Kluyveromyces, are reported. Two main groups of mutually interfertile taxa were established within the genus. The first group comprises Kluyveromyces bulgaricus, Kluyveromyces cicerisporus, Kluyveromyces dobzhanskii, Kluyveromyces drosophilarum, Kluyveromyces fragilis, Kluyveromyces lactis, Kluyveromyces marxianus, Kluyveromyces phaseolosporus, Kluyveromyces vanudenii and Kluyveromyces wikenii. The second group consists of Kluyveromyces dabzhanskii, Kluyveromyces drosophilarum, Kluyveromyces laotis, Kluyveromyces vanudenii and Kluyveromyces wiokerhamii. Hybrids were also detected in crosses involving Kluyveromyces drosophilarum and Kluyveromyces waltii as well as Kluyveromyces marxianus and Kluyveromyces thermotolerans. In terms of the concept of the biological species and in compliance with the requirements of the International Code of Botanical Nomenclature, taxa which hybridize with Kluyveromyces marxianus and form fertile recombinants at frequencies observed in intraspecific crosses, are accepted as varieties of Kluyveromyces marxianus. Hybridization was observed between Kluyveromyces marxianus var. lactis and the presumed imperfect forms of some Kluyveromyces species, namely Candida kefyr, Candida macedoniensis and Torulopsis sphaerica. Recombination was not detected in crosses involving Kluyveromyces marxianus var. marxianus and representatives of other yeast genera, i.e. Pichia, Saccharomyces, Torulaspora and Zygosaccharomyces. Conclusions regarding the relationship between members of the genus Kluyveromyces, reached on the basis of this investigation are compared with those reported by other workers, who based their investigations on phenotypic characteristics as well as on the determinations of mol % G+C and DNA-DNA homology studies.

No description available.

No description available.

The aim of this work was to establish the relative contribution of genetic regulation of the <I>PYK1</I>, <I>PFK1</I> and <I>PFK2</I> genes to the control of glycolysis. A series of isogenic mutant strains were constructed where the promoters and 5' untranslated sequences of the <I>PYK1, PFK1</I> and <I>PFK2</I> genes were replaced with those from <I>PGK1</I>. In addition , a second series of mutant strains were constructed where synthesis of Pyk1p and Pflkp was driven by the <I>PGK1_Δuas</I> promoter. These latter series of mutants were designed to contain weak expression of Pyklp and Pflkp. Analysis of UKC1 (<I>PGK::PYK1)</I> in shake flask cultures revealed similar growth rates on glucose and on lactate and similar rates of ethanol production and glucose consumption to those of the wild-type strain. This suggested that the native genetic regulation did not appear to play a significant role in the control of glycolysis. Nonetheless, analysis of this strain in the fermentor revealed that genetic regulation of <I>PYK1</I> may be important in co-ordinating Pyk1p synthesis, under the conditions studied. Analysis of YKC11 (<I>PGK_Δuas::PYK1)</I> in both shake flask and fermentor experiments showed that genetic control was important in maintaining Pyk1p levels in order to sustain glycolytic flux. Shake flask analysis of the single and double <I>PFK</I> mutants under the control of the <I>PGK1</I> promoter revealed that the genetic regulation of the <I>PFK1</I> and <I>PFK2</I> genes did not appear to be important in the control of glycolysis. Weak expression of the <I>PFK1</I> and <I>PFK2</I> genes, under the control of the <I>PGK_Δuas</I> promoter showed the importance of genetic regulation in maintaining Pflkp levels to support glycolytic flux, under the conditions studied.

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2007. / Title from title screen (site viewed Mar. 27, 2008). PDF text: xxiii, 235 p. : ill. (some col.) ; 3 Mb. UMI publication number: AAT 3284719. Includes bibliographical references. Also available in microfilm and microfiche formats.

Thesis (M.Sc. Hons.) -- University of Western Sydney, Hawkesbury, 1999. / Thesis submitted for the degree of Master of Science (Hons.). Includes bibliographical references (leaves 69-75).