The term Traditional Ecological Knowledge (TEK) is generally interpreted as the knowledge held by Indigenous communities about their environment and the cultural frameworks in which such knowledge is transmitted. There is no universally accepted definition of TEK and interpretations can vary between Western and Indigenous lenses. TEK as an academic field has gained a vast literature base; however, there is a dearth of literature in the wildlife field that expresses TEK through an Indigenous lens. There has been growing attention on TEK in the wildlife profession, warranting further exploration of how Indigenous and Western scientific paradigms may be used together in natural resources conservation. Herein, I present my doctoral research, where I focused on a culturally sensitive approach in the pursuit of TEK studies. I conducted interdisciplinary research with the Yurok Tribe of northwestern California in two parts: (1) a human dimensions study where I interviewed Yurok people regarding TEK and their relationship with wildlife and (2) a wildlife survey on Yurok ancestral lands where I used genetic analyses of scats as a noninvasive method for determining species presence and diet. In addition, I developed a synthesis document regarding historical aspects of Indian Country in the United States and philosophical contexts of TEK as science to facilitate dialogue regarding cultural sensitivity in wildlife research with a TEK component. During an internship with the National Park Service (NPS), I developed a guidance document to provide resources regarding TEK in wildlife conservation and a case study detailing how I navigated my doctoral research. My dissertation consists of five manuscripts, each formatted for a specific journal or the NPS.