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Epidermal growth factor network in zebrafish ovary.January 2008 (has links)
Tse, Chung Kwan Anna. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 86-105). / Abstracts in English and Chinese. / Abstract in English --- p.i / Abstract in Chinese --- p.iii / Acknowledgement --- p.v / Table of content --- p.vii / List of figures and tables --- p.xi / Symbols and abbreviation --- p.xiii / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Folliculogenesis --- p.1 / Chapter 1.1.1 --- In Mammals --- p.1 / Chapter 1.1.2 --- In Teleosts --- p.2 / Chapter 1.1.2.1 --- Vitellogenesis --- p.3 / Chapter 1.1.2.2 --- Oocyte Maturation --- p.4 / Chapter 1.1.3 --- Oocyte Control of Folliculogenesis --- p.5 / Chapter 1.2 --- Epidermal Growth Factor Family --- p.7 / Chapter 1.2.1 --- Epidermal Growth Factor Ligand Family --- p.7 / Chapter 1.2.1.1 --- Discovery --- p.7 / Chapter 1.2.1.2 --- Structure --- p.8 / Chapter 1.2.1.3 --- Shedding --- p.8 / Chapter 1.2.1.4 --- Functions --- p.9 / Chapter 1.2.2 --- Epidermal Growth Factor Receptor Family --- p.10 / Chapter 1.2.2.1 --- Structure --- p.10 / Chapter 1.2.2.2 --- Ligand Binding and Dimerization --- p.11 / Chapter 1.2.2.3 --- Signaling and Internalization --- p.12 / Chapter 1.2.3 --- EGF Family in Reproduction --- p.13 / Chapter 1.2.3.1 --- Reproductive Function of EGF Family --- p.13 / Chapter 1.2.3.2 --- EGF Network as Downstream Mediator of Gonadotropins --- p.15 / Chapter 1.3 --- Objectives of the Present Study --- p.18 / Chapter Chapter 2 --- Spatiotemporal Expression of the EGF Family in Zebrafish Ovary / Chapter 2.1 --- Introduction --- p.20 / Chapter 2.2 --- Materials and Methods --- p.22 / Chapter 2.2.1 --- Chemicals --- p.22 / Chapter 2.2.2 --- Animals --- p.22 / Chapter 2.2.3 --- Isolation of Different Tissues and Ovarian Follicles --- p.22 / Chapter 2.2.4 --- Separation of Oocyte and Follicle layers --- p.23 / Chapter 2.2.5 --- Embryo Collection --- p.23 / Chapter 2.2.6 --- Tyrphostin AG 1478 Treatment of Embryos --- p.23 / Chapter 2.2.7 --- Total RNA Isolation and Reverse Transcription --- p.24 / Chapter 2.2.8 --- Semi-quantitative and Real-time Polymerase Chain Reaction (PCR) --- p.24 / Chapter 2.2.9 --- Data Analysis --- p.25 / Chapter 2.3 --- Results --- p.27 / Chapter 2.3.1 --- Validation of Semi-quantitative PCR Quantification --- p.27 / Chapter 2.3.2 --- Tissue Distribution of the EGF Family --- p.27 / Chapter 2.3.3 --- Spatial Expression of the EGF and the Activin Families within the Follicles --- p.27 / Chapter 2.3.4 --- Temporal Expression of the EGF Family in Folliculogenesis --- p.29 / Chapter 2.3.5 --- Temporal Expression of the EGF Family in Embryogenesis --- p.29 / Chapter 2.3.6 --- Effect of Blocking EGFR on Embryonic Development --- p.30 / Chapter 2.4 --- Discussion --- p.31 / Chapter Chapter 3 --- Regulation of the EGF Family in Cultured Follicle Cells and Their Effects on the Expression of Activin Subunits / Chapter 3.1 --- Introduction --- p.55 / Chapter 3.2 --- Materials and Methods --- p.58 / Chapter 3.2.1 --- Chemicals --- p.58 / Chapter 3.2.2 --- Primary Follicle Culture --- p.58 / Chapter 3.2.3 --- Ovarian Fragment Incubation --- p.58 / Chapter 3.2.4 --- Total RNA Isolation and Reverse Transcription --- p.58 / Chapter 3.2.5 --- Semi-quantitative and Real-time Polymerase Chain Reaction (PCR) --- p.59 / Chapter 3.2.6 --- Microinjection --- p.59 / Chapter 3.2.7 --- Data analysis --- p.59 / Chapter 3.3 --- Results / Chapter 3.3.1 --- Expression of the EGF Family in Cultured Follicle Cells --- p.61 / Chapter 3.3.2 --- Effects of EGF on the EGF Family Expression in Cultured Follicle Cells --- p.61 / Chapter 3.3.3 --- Effects of BTC and HB-EGF on the Expression of EGF Ligands in Cultured Follicle Cells --- p.61 / Chapter 3.3.4 --- Effect of EGF Ligands on the Expression of Activin Subunits in Cultured Follicle Cells --- p.62 / Chapter 3.3.5 --- Effects of Actinomycin D on Ovarian Fragments --- p.62 / Chapter 3.3.6 --- Effects of Microinjecting Anti-EGF Morpholino on Full Grown Follicles --- p.62 / Chapter 3.4 --- Discussion --- p.64 / Chapter Chapter 4 --- General Discussion --- p.81 / References --- p.86
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Inhibin: its presence, regulation and function in the zebrafish, Danio rerio.January 2007 (has links)
Poon, Shui-Kei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 78-98). / Abstracts in English and Chinese. / Abstract (in English) --- p.ii / Abstract (in Chinese) --- p.iv / Acknowledgement --- p.vi / Table of content --- p.vii / List of figures --- p.x / Symbols and abbreviations --- p.xii / Chapter 1 General Introduction / Chapter 1.1 --- Structure of ovarian follicles --- p.1 / Chapter 1.2 --- Folliculogenesis and its control --- p.1 / Chapter 1.2.1 --- History of inhibin discovery --- p.4 / Chapter 1.2.2 --- Inhibin and its related proteins --- p.5 / Chapter 1.3 --- Inhibin --- p.6 / Chapter 1.3.1 --- Structure --- p.6 / Chapter 1.3.2 --- Function --- p.7 / Chapter 1.3.3 --- Signaling --- p.11 / Chapter 1.3.4 --- Expression --- p.14 / Chapter 1.3.5 --- Regulation --- p.15 / Chapter 1.4 --- Objectives of the present study --- p.19 / Chapter Chapter 2 --- Spatiotemperoal Expression Profiles of Inhibin a in the Zebrafish Ovary / Chapter 2.1 --- Introduction --- p.25 / Chapter 2.2 --- Materials and Methods --- p.27 / Chapter 2.2.1 --- Animals --- p.27 / Chapter 2.2.2 --- Chemicals --- p.27 / Chapter 2.2.3 --- Separation of oocytes and follicular layers --- p.28 / Chapter 2.2.4 --- Isolation of ovarian follicles --- p.28 / Chapter 2.2.5 --- RNA isolation and reverse transcription --- p.28 / Chapter 2.2.6 --- Real-time and semi-quantitative RT-PCR quantification of expression --- p.29 / Chapter 2.2.4 --- Data analysis --- p.30 / Chapter 2.3 --- Results --- p.30 / Chapter 2.3.1 --- Validation of semi-quantitative RT-PCR quantification --- p.30 / Chapter 2.3.2 --- Tissue distribution of inha expression --- p.30 / Chapter 2.3.3 --- Localization of inha expression within the ovarian follicle --- p.31 / Chapter 2.3.4 --- Further evidence for inha expression in the follicle layer --- p.31 / Chapter 2.3.5 --- Stage-dependent expression of inha in the ovarian follicles --- p.32 / Chapter 2.4 --- Discussion --- p.33 / Chapter Chapter 3 --- Regulation of Inhibin a Expression in vitro and in vivo and the Effect of Inhibin on Final Oocyte Maturation / Chapter 3.1 --- Introduction --- p.43 / Chapter 3.2 --- Materials and Methods --- p.46 / Chapter 3.2.1 --- Animals --- p.46 / Chapter 3.2.2 --- Chemicals and hormones --- p.46 / Chapter 3.2.3 --- Preparation of goldfish pituitary extract --- p.47 / Chapter 3.2.4 --- Ovarian fragment incubation --- p.47 / Chapter 3.2.5 --- Preparation of spontaneously matured follicles --- p.48 / Chapter 3.2.6 --- Intra-peritoneal injection --- p.48 / Chapter 3.2.7 --- RNA isolation and reverse transcription --- p.48 / Chapter 3.2.8 --- Real-time and semi-quantitative RT-PCR quantification of expression --- p.48 / Chapter 3.2.9 --- Data analysis --- p.49 / Chapter 3.3 --- Results --- p.49 / Chapter 3.3.1 --- Validation of semi-quantitative RT-PCR quantification --- p.49 / Chapter 3.3.2 --- Temporal change of basal inha expression in cultured ovarian fragments --- p.49 / Chapter 3.3.3 --- Effect of pituitary extract on the expression of inha --- p.50 / Chapter 3.3.4 --- Effects of recombinant zebrafish FSH and LH on inha expression --- p.51 / Chapter 3.3.5 --- Effect of forskolin on inha expression --- p.52 / Chapter 3.3.6 --- Involvement of PKA and p38MAPK in the pituitary extract and forskolin-induced up-regulation of inha expression --- p.52 / Chapter 3.3.7 --- Effect of recombinant zfFSH on the expression of inha in vivo --- p.53 / Chapter 3.3.8 --- Effects of inhibin on the basal and DHP-induced final oocyte maturation --- p.53 / Chapter 3.4 --- Discussion --- p.54 / Chapter Chapter 4 --- General Discussion --- p.71 / References --- p.78
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Aflatoxin B��� metabolism, CYP1A induction, and the influence of CYP1A induction on aflatoxin B��� metabolism in zebrafishTroxel, Claudia McLaughlin 03 October 1996 (has links)
Graduation date: 1997
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Characterization of HSCs in zebrafish using label-retaining strategy /Zhang, Jianbing. January 2009 (has links)
Includes bibliographical references (p. 80-91).
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Using zebrafish to identify new regulators of haematopoiesisSerbanovic-Canic, Jovana January 2013 (has links)
No description available.
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Identification and functional analysis of Zebrafish orthologs of genesChalla, Anil Kumar. January 2003 (has links)
Thesis (Ph. D.)--Ohio State University, 2003. / Document formatted into pages; contains 119 p. Includes bibliographical references. Abstract available online via OhioLINK's ETD Center; full text release delayed at author's request until 2005 Aug. 19.
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A study of the role of calcium ions during cytokinesis in cleavage stage zebrafish embryos /Lee, Karen Wing-man. January 2004 (has links)
Thesis (Ph.D.)--Hong Kong University of Science and Technology, 2004. / Includes bibliographical references (leaves 192-206). Also available in electronic version. Access restricted to campus users.
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The role of matrix metalloproteinases in zebrafish (danio rerio) embryogenesis and their regulation by glucocorticoidsHillegass, Jedd Michael. January 2008 (has links)
Thesis (Ph. D.)--Rutgers University, 2008. / "Graduate Program in Toxicology." Includes bibliographical references (p. 135-152).
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Spatiotemporal analysis of apoptosis patterns in the developing brain of the Brd2-knockdown zebrafish embryoMelville, Heather. January 2009 (has links)
Thesis (M.S.)--Villanova University, 2009. / Biology Dept. Includes bibliographical references.
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Sodium channels are required for cardiac cell-fate specification via a novel, non-electrogenic mechanism in zebrafishChopra, Sameer, January 1900 (has links)
Thesis (Ph. D. in Pharmacology)--Vanderbilt University, Dec. 2008. / Title from title screen. Includes bibliographical references.
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