Thesis (Ph. D.)--Ohio State University, 2008.

Thesis (Ph. D.)--University of Chicago, Committee on Developmental Biology, March 2003. / Includes bibliographical references. Also available on the Internet.

Thesis (Ph. D.)--University of Oregon, 1999. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 63-69). Also available for download via the World Wide Web; free to University of Oregon users. Address: http://wwwlib.umi.com/cr/uoregon/fullcit?p9957571.

Thesis (Ph. D.)--Virginia Commonwealth University, 2010. / Prepared for: Dept. of Life Sciences. Title from resource description page. Includes bibliographical references. Unavailable until 5/12/2015.

Thesis (Ph.D.)--Aberdeen University, 1989. / Title from web page (viewed on Mar. 4, 2010). Accompanying offprints stored in end pocket. With: A combined radio and acoustic transmitter for fixing direction and range of freshwater fish (RAFIX) / J.D. Armstrong ... et al. J. Fish Biol. 33, 879-884. With: An acoustic telemetry system for monitoring the heart rate of pike, Esox Lucius L. and other fish in their natural environment / J.D. Armstong ... et. J. Exp Bio 1989: 143, 549-552. With: Effects of implanted dummy transmitters on mortality, growth and tissue reaction in rainbow trout, Salmo gairdneri / M.C. Lucas . J. Fish Bio. 35, 577-587. Includes bibliographical references.

Thesis (M.S.)--Washington State University, May 2006. / Includes bibliographical references (p. 54-57).

Thesis (Ph. D. in Cell and Developmental Biology)--Vanderbilt University, May 2006. / Title from title screen. Includes bibliographical references.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Includes bibliographical references (p. 137-161).

Thesis (M.S. in biochemistry)--Washington State University, December 2007. / Includes bibliographical references (p. 36-47).

The proteome is in constant flux and therefore it is important to understand the contribution of protein dynamics to the function of an organism. The zebrafish (Danio rerio) is a recognised model organism that is widely used to investigate physiological processes. The focus of this thesis was to develop a method to calculate the rates of synthesis of heart proteins of zebrafish on a proteome-wide scale. The initial stage of the project involved the optimisation of a method to characterise the protein complement of individual zebrafish hearts. It was concluded that for the rapid screening of proteins 1-dimensional gel electrophoresis in conjunction with high resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) was the most appropriate experimental approach. In order to determine the rates of protein synthesis, zebrafish were administered with a stable isotope-labelled amino acid ([2H7] L-leucine) via the diet and its incorporation into heart proteins was monitored over an 8 week time course. Using this method it was possible to calculate the synthesis rates of over 600 proteins. The experimental strategy was then applied to define the changes in protein synthesis rates in hearts from zebrafish that were subjected to chronic unpredictable stress (CUS). A variety of stressors on zebrafish comprising air emersion, net chasing or net confinement were employed to model CUS. These approaches were validated by a parallel behavioural analysis. The results revealed that glycolytic and gluconeogenic enzymes as well as proteins involved in hypoxia had significantly altered synthesis rates in response to induced stress. This thesis describes for the first time a proteomics approach to determine the rates of synthesis of individual proteins in the zebrafish and its application to investigate the effects of stress conditions on heart proteome dynamics.