Bacteria were isolated from water at Alder Slough, Oregon. Of 15 isolates grown successfully in the medium employed, 100% showed measurable uptake of Zn-65. A growth curve was established for one isolate, a gram-negative rod designated AS-1. Increasing Zn-65 uptake was found generally to correspond with increasing growth of AS-1. The optimum growth temperature for this isolate was 31°C, with very little growth at 37.5°C and 6.7°C. Growth and Zn-65 uptake occurred in a 35 ppt medium from a temperature of 31.0°C to 13.9°C but little growth and no Zn-65 uptake was observed at 8.6°C and 4.4°C. Growth and Zn-65 uptake in a 0 ppt medium occurred from 31.0°C to 5.0°C, with little growth but measurable Zn-65 uptake at 8.9°C and 5.0°C. It was found that 200 ppm Mg added to a culture which had already taken up Zn-65 failed to displace the zinc from the cells. A interaction of the energy source, Casamino Acids, with the Zn-65 spike influenced the results. When cells were lysed, spiked with Zn-65, and the debris removed by centrifugation, 84.8% of the Zn-65 remained in the supernatant. But when cells grown first in Zn-65 spiked medium were lysed and centrifuged, 74.4% of the Zn-65 was found in the debris. It was concluded that bacterial uptake of Zn-65 and other metal cations must be considered in determining the fates of these materials released into the environment. Chemical adsorption phenomena were determined to be a major factor controlling this uptake, with other chemical and biological factors, such as competitive binding by the medium and active uptake or exclusion by the cells, exerting a significant influence that requires further investigation to characterize. / Graduation date: 1971

Thesis (Ph. D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xiii, 155 p.: ill (some col.). Includes abstract and vita. Advisor: Tammy Bray, Nutrition Program. Includes bibliographical references (p. 139-155).

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. [119]-125.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1963. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--Wisconsin. / Includes bibliographical references (leaves 84-112).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1975. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

This research was designed to study the perinatal and postweaning effects of the interaction between ethanol and low dietary zinc during gestation and lactation in the rat. Pregnant rats were fed liquid diets containing either 2 or 10 μg zinc/ml with or without 30% of kcal from ethanol throughout gestation and lactation. The liquid diet formulation was nutritionally adequate to insure offspring growth and survival during lactation. At weaning, dams and five of eight offspring from each litter were killed by exsanguination under sodium pentobarbital anesthesia. The remaining offspring were orally inoculated with Streptococcus mutans and fed a caries-promoting diet for six weeks. The low zinc diet produced a moderate zinc deficiency in dams as evidenced by a decrease in tissue zinc content, serum alkaline phosphatase activity, and urinary zinc concentration. Despite the presence of high zinc content in the diet, ethanol antagonized maternal zinc status to a level typical of that produced by the low zinc diet. The lowest zinc status, however, was found when low dietary zinc and ethanol were combined. The maternal interaction between ethanol and zinc also depressed offspring serum zinc and alkaline phosphatase activity in a similar manner but the magnitude was smaller. The maintenance of a lower than normal maternal tissue zinc and decreased maternal urinary excretion of zinc suggested a maternal attempt to support the growth and development of offspring despite zinc deficiency. Physiological consequences of ethanol-antagonized zinc status were evidenced by depressed activity of maternal and offspring serum alkaline phosphatase, increased maternal urinary excretion of hydroxyproline, decreased offspring molar enamel and dentin zinc content, increased dental caries score, and decreased cross-linking structure of mandibular second molar enamel. The liquid diet developed in the present study was nutritionally adequate and allowed for the investigation of a single nutrient deficiency, zinc, in ethanol fed rats during gestation and lactation without confounding effects of general malnutrition. Although the direction of interaction was predominately an effect of ethanol on zinc rather than the effect of zinc on ethanol, this study clearly indicates that zinc deficiency is an important consequence of maternal ethanol ingestion. / Graduation date: 1984

The specific activity of ⁶⁵Zn (nanocuries ⁶⁵Zn/g total Zn) was determined in various organs of the common coastal mussel, Mytilus californianus Conrad, collected from six locations along the Pacific Coast. These organs included the gills, mantle, foot, reproductive organs, adductor muscle, and viscera. After ashing and dissolving with nitric acid (8 M), each tissue was analyzed for ⁶⁵Zn by gamma-ray spectrometry. The determination of total zinc concentrations of the various organs was accomplished by atomic absorption spectrophotometry as well as by neutron activation analysis. Variable amounts of both stable zinc and radioactive ⁶⁵Zn were observed within the specific tissues from a given location. The ⁶⁵Zn specific activities of the tissues of organisms from a particular station however, tended to be uniform, at least within the uncertainty of the measurements. Both radiozinc and ⁶⁵Zn specific activity decreased with distance from the Columbia River mouth, whereas the stable zinc tended to remain uniform for a specific tissue. Tissues high in radiozinc were also high in stable zinc and conversely. An estimate of the input specific activity from the mussel' environment (food and/or water) was calculated from a simple model resulting in a value similar to zooplankton values sampled from within the Columbia River plume. Comparison of the concentrations of zinc determined by neutron activation with those determined by atomic absorption spectrophotometry indicated a linear relationship between the two methods. Results of atomic absorption measurements were approximately 27% larger than the results of neutron activation analysis, indicating the presence of a systematic error. The higher values attained by atomic absorption are attributed to evaporation during storage of the ash solution which would lead to an increase in zinc concentration. In considering the two methods of analysis, economy of both time and expenses favors the atomic absorption method over that of neutron activation. / Graduation date: 1971

Includes bibliographical references (leaves 234-280) / xxviii, 292, [72] leaves : ill. (some col.), plates (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 2002

Thesis (M.S.)--Rutgers University, 2009. / "Graduate Program in Chemistry." Includes bibliographical references (p.54-56).