Thesis (M.S.)--University of Wisconsin--Madison, 1937. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 19-20).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1965. / Typescript. Vita. Description based on print version record. Includes bibliographical references.

No abstract available. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1975.

by Tsang Yuen Shan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 98-110). / ACKNOWLEDGMENTS --- p.I / ABSTRACT --- p.II / CONTENTS --- p.IV / ABBREVIATIONS --- p.VII / LIST OF FIGURES --- p.IX / LIST OF TABLES --- p.XII / Chapter 1. --- INTRODUCTION --- p.1 / Chapter 1.1 --- Biochemistry of zinc --- p.1 / Chapter 1.2 --- Zinc within the body --- p.2 / Chapter 1.2.1 --- Roles of zinc in general biochemical processes --- p.4 / Chapter 1.2.2 --- Zinc and zinc-binding proteins (ZnBPs) --- p.9 / Chapter 1.3 --- Zinc in brain --- p.12 / Chapter 1.3.1 --- Distribution of zinc in brain --- p.13 / Chapter 1.3.2 --- Roles of zinc in brain 、 --- p.14 / Chapter 1.4 --- Summary --- p.18 / Chapter 1.5 --- Aim of Study --- p.20 / Chapter 2. --- MATERIALS AND METHODS --- p.22 / Chapter 2.1 --- Detection of zinc binding proteins --- p.22 / Chapter 2.1.1 --- Experimental animal --- p.22 / Chapter 2.1.2 --- Subcellular fractionation of porcine brain --- p.22 / Chapter 2.1.2 --- Determination of protein concentration --- p.24 / Chapter 2.1.3 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) --- p.24 / Chapter 2.1.4 --- Two dimensional polyacrylmide gel electrophoresis (2D-PAGE) --- p.25 / Chapter 2.1.5 --- "Fixing, staining and destaining" --- p.28 / Chapter 2.1.6 --- Western blotting for SDS-PAGE --- p.29 / Chapter 2.1.7 --- 65Zn binding --- p.30 / Chapter 2.1.8 --- Autoradiography --- p.30 / Chapter 2.1.9 --- Data quantification --- p.31 / Chapter 2.2 --- Identification of the 21 kD ZnBP --- p.31 / Chapter 2.2.1 --- N-terminal sequencing of the 21 kD ZnBP --- p.31 / Chapter 2.2.2 --- In situ cyanogen bromide (CNBr) cleavage --- p.32 / Chapter 2.2.3 --- Tricine SDS-PAGE --- p.33 / Chapter 2.2.4 --- Isolation of myelin --- p.34 / Chapter 2.2.5 --- Extraction of the 21 kD ZnBP --- p.35 / Chapter 2.2.6 --- Comparison of the mobility of the 21 kD ZnBP with that of bovine myelin basic protein (MBP) in three different gel electrophoresis system --- p.35 / Chapter 2.3 --- Characterization of the zinc binding properties of MBP --- p.38 / Chapter 2.3.1 --- Concentration-dependence of MBP on 65Zn binding --- p.38 / Chapter 2.3.2 --- Effect of other divalent cations on 65Zn binding to the 21 kD ZnBP --- p.38 / Chapter 2.3.3 --- Effect of pH on 65Zn binding to the 21 kD ZnBP --- p.39 / Chapter 2.3.4 --- Effect of modification of histidine residues in MBP on 65Zn binding --- p.39 / Chapter 2.4 --- Effect of zinc on the interaction between MBP and membrane- lipid --- p.40 / Chapter 2.4.1 --- Effect of zinc on MBP-induced phospholipid vesicle aggregation --- p.40 / Chapter 2.4.2 --- Effect of some divalent cations on MBP-induced phospholipid vesicle aggregation --- p.41 / Chapter 3. --- RESULTS --- p.42 / Chapter 3.1 --- Distribution of ZnBPs in porcine brain --- p.42 / Chapter 3.1.1 --- Subcellular distribution of ZnBPs in porcine brain --- p.42 / Chapter 3.1.2 --- "Comparison of the subcellular distribution of ZnBPs in brain, heart and liver" --- p.45 / Chapter 3.1.3 --- Regional distribution of ZnBPs in porcine brain --- p.48 / Chapter 3.1.4 --- Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of total brain homogenates --- p.51 / Chapter 3.2 --- Identification of the 21 kD ZnBP --- p.58 / Chapter 3.2.1 --- N-terminal sequencing of the 21 kD ZnBP --- p.58 / Chapter 3.2.2 --- CNBr cleavage of the 21 kD ZnBP and the N-terminal sequencing of CNBr cleaved fragment --- p.59 / Chapter 3.2.3 --- Detection of the 21 kD ZnBP in myelin fraction and extraction of the 21 kD ZnBP by chloroform-methanol extraction --- p.59 / Chapter 3.2.4 --- Comparison of properties of the 21 kD ZnBP with bovine MBP on three electrophoresis systems --- p.61 / Chapter 3.2.5 --- CNBr cleavage patterns of the 21 kD ZnBP and the bovine MBP --- p.64 / Chapter 3.3 --- Characterization of the zinc binding properties of MBP --- p.69 / Chapter 3.3.1 --- Concentration-dependence of MBP on 65Zn binding --- p.69 / Chapter 3.3.2 --- Effect of other divalent cations on 65Zn binding to the 21 kD ZnBP --- p.69 / Chapter 3.3.3 --- Effect of pH on 65Zn binding to the 21 kD ZnBP --- p.71 / Chapter 3.3.4 --- Effect of modification of histidine residues in MBP on 65Zn binding --- p.73 / Chapter 3.4 --- Effect of zinc on the interaction between MBP and membrane- lipid --- p.76 / Chapter 3.4.1 --- Effect of zinc on MBP-induced phospholipid vesicle aggregation --- p.76 / Chapter 3.4.2 --- Effect of other divalent cations on MBP-induced phospholipid vesicle aggregation --- p.81 / Chapter 4. --- DISCUSSION --- p.83 / Chapter 5. --- CONCLUSIONS --- p.96 / Chapter 6. --- REFERENCES --- p.98

Includes bibliographical references (leaves 234-280) / xxviii, 292, [72] leaves : ill. (some col.), plates (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Medicine, 2002

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No / (51)Cr-labeled, superparamagnetic, iron oxide nanoparticles ((51)Cr-SPIOs) and (65)Zn-labeled CdSe/CdS/ZnS-quantum dots ((65)Zn-Qdots) were prepared using an easy, on demand, exchange-labeling technique and their particokinetic parameters were studied in mice after intravenous injection. The results indicate that the application of these heterologous isotopes can be used to successfully mark the nanoparticles during initial distribution and organ uptake, although the (65)Zn-label appeared not to be fully stable. As the degradation of the nanoparticles takes place, the individual transport mechanisms for the different isotopes must be carefully taken into account. Although this variation in transport paths can bring new insights with regard to the respective trace element homeostasis, it can also limit the relevance of such trace material-based approaches in nanobioscience. By monitoring (51)Cr-SPIOs after oral gavage, the gastrointestinal non-absorption of intact SPIOs in a hydrophilic or lipophilic surrounding was measured in mice with such high sensitivity for the first time. After intravenous injection, polymer-coated, (65)Zn-Qdots were mainly taken up by the liver and spleen, which was different from that of ionic (65)ZnCl2. Following the label for 4 weeks, an indication of substantial degradation of the nanoparticles and the release of the label into the Zn pool was observed. Confocal microscopy of rat liver cryosections (prepared 2 h after intravenous injection of polymer-coated Qdots) revealed a colocalization with markers for Kupffer cells and liver sinusoidal endothelial cells (LSEC), but not with hepatocytes. In J774 macrophages, fluorescent Qdots were found colocalized with lysosomal markers. After 24 h, no signs of degradation could be detected. However, after 12 weeks, no fluorescent nanoparticles could be detected in the liver cryosections, which would confirm our (65)Zn data showing a substantial degradation of the polymer-coated CdSe/CdS/ZnS-Qdots in the liver.

No description available.