Acid whey is a by-product of the quarg production and arises in large volumes in dairies. A considerable disposal problem arises due to the lack of obtainable proceeds from acid whey utilisation. Additionally, sustainable and energy efficient treatment methods for high strength liquid wastes from dairies cleaning operation are needed to reduce the costs of wastewater treatment. Samples of acid whey and spent cleaning solutions from a quarg cheese production plant were collected. The composition and physical properties were analysed and evaluated against waste treatment process requirements. The occurrence of different waste streams, their volumes and frequencies were also investigated. A laboratory scale membrane nanofiltration plant was designed, and built for investigation of the volume reduction of cleaning process effluents with emphasis to treatment options for the filtration concentrates. The examination of the rheological properties of alkaline CIP wastewaters at different volume reduction ratios clearly shows that these effluents are Newtonian fluids even at high concentrations. The anaerobic biodegradability of acid whey and mixtures containing portions of alkaline CIP wastewaters at different volume reduction ratios was tested. Characteristic process kinetics for acid whey fermentation in batch mode was observed. The occurrence of a second lag-phase in mixtures containing larger portions of acid whey was identified as phase separation- due to rapid acidification of lactose. Anaerobic digestion (AD) was identified as a suitable treatment option for acid whey and alkaline CIP wastewaters. Four anaerobic digester types were designed with regard to their suitability for high strength waste treatment and were built and operated at laboratory scale. The reactors tested were: a) A Continuous Stirred Tank Reactor (CSTR); b) An Anaerobic Membrane Reactor (AMR); c) An Upflow Anaerobic Sludge Blanket (UASB) re- actor; and d) A novel two-stage process design consisting of a combined acidification and crystallization stage and a gaslift driven fluidised bed methanogenic stage. The operation of the AMR process and also of the UASB process with internal circulation and pH-control using alkaline CIP effluents was evaluated at high loading rates of 7.7 g•L-1•d-1 and 10.2 g•L-1•d-1 respective. However, in the experiments it was demonstrated that even with perfect biomass retention the operation of one stage anaerobic digestion at high loading rates caused process upsets. Precipitation and accumulation of milk minerals within the sludge was observed in all one stage experiments. The conclusions drawn from one stage studies led to the design of a novel high-rate diges- tion system to meet the demands of anaerobic digestion of acid whey and effluents from dairy plant cleaning. The design based on different high-rate industrial reactor designs and incorporate the ideas of staging, crystallisation of calcium salts prior to anaerobic di- gestion, fluidised bed and internal circulation reactors, and also jet-loop or gaslift reactors. The performance of the novel system when treating acid whey is comparable to the results of well designed, two-stage digesters treating cheese whey which is easier to digest.

Acid whey, a byproduct of Greek yogurt production, has little commercial value due to its low protein content and is also environmentally harmful when disposed of as waste. However, as a product of microbial fermentation, acid whey could be a rich source of beneficial metabolites associated with fermented foods. This study increases understanding of acid whey composition by providing a complete metabolomic profile of acid whey. Commercial and lab-made Greek yogurts, prepared with three different bacterial culture combinations, were evaluated. Samples of unfermented yogurt mix and cultured whey from each batch were analyzed. Ultra-high-performance liquid chromatography/tandem mass spectrometry metabolomics were employed to separate and identify 477 metabolites, including many with potential health benefits similar to those provided by yogurt, such as creatine and acetylcarnitine. Examples of other metabolites identified in the acid whey include beneficial phospholipids (1,2-dilinoleoyl-sn-glycero-3-phosphocholine) and sphingolipids; compounds with neuroprotective (glycerophosphorylcholine) or cardiovascular (betaine) benefits; antimicrobial compounds (benzoic acid), and anti-inflammatory compounds (citrulline). Compared to uncultured controls, acid whey showed decreases in some metabolites associated with microbial metabolism and increases in others. Metabolite production was significantly affected by combinations of culturing organisms, and production location. Differences between lab-made and commercial samples could be caused by different starting ingredients, or environmental factors or both.

No description available.

No description available.

No description available.

Made available in DSpace on 2017-07-21T18:53:24Z (GMT). No. of bitstreams: 1 Paola Lievore.pdf: 889646 bytes, checksum: 86e0b724d1cb1457641fa384d7c5ab5a (MD5) Previous issue date: 2013-02-27 / Acid whey is generated in large volumes as a by-product of the Quark cheese production. Quark cheese is the base for the Petit Suisse production. This acid whey has a potential use in the preparation of beverages due to its high nutritional value. It is rich in minerals with a high biological value and a source of protein. Fermented milk products are well accepted by consumers due to their probiotic properties, coming mainly from the specific lactic acid bacteria used in these products, providing health benefits. In this context, the use of liquid acid whey as a base for the preparation of fermented milk has shown to be an interesting alternative. Apart from the functional and nutritional enrichment of the fermented dairy beverage, coming from the acid whey, this use avoid the need of other conversion processes to get rid of it, reducing the environmental problems related to its disposal. This study aims to characterize the physicochemical properties of the acid whey resulting from the manufacture of Quark cheese, base of the Petit Suisse, and use it as an ingredient for the manufacture of fermented milk. The acid whey was collected fresh in the industry under study and subjected to analysis of pH, acidity, lactose content, fat, protein, moisture and solids during the period from November 2011 until November 2012. The fermented milk was prepared using the acid whey in the place of water in the original formula and reduction of fermentation time from 60 for 25 hours. All ingredients were blended with a mixer and pasteurized; the liquor was then characterized with respect to pH, Brix, acidity, protein, lactose, and reducing sugars. The product was subjected to multiple comparison tests using as standard the commercial product. Acceptance test and purchase intent were carried out. The values of the physicochemical evaluations of acid whey were treated statistically by Fisher Test, considering the mean and standard deviation. The results of the multiple comparison tests were analyzed by Variance Analysis. The results of physicochemical analyzes of acid whey show that the averages were similar to those found by several authors, but the protein contents were slightly higher. Significant differences were found between summer and winter for all parameters except lactose. The highest content of minerals, lactose and consequently total solids were observed during autumn. The multiple comparison tests showed that the fermented milk was considered by all trained judges as worse than the standard. Acceptance testing resulted in a 90 %acceptance, with averages of responses between the terms of the hedonic scale "liked very much" and "liked moderately". In the analysis of purchase intent it was found that 54 % of consumers who participated in the tasting would certainly buy the product and 38 % would like to buy. The use of acid whey for production of fermented milk proved feasible, besides adding value to the product under study allows the industry to reduce the discharge of acid whey as well as the water consumption. / O soro ácido é um subproduto da indústria de fabricação de queijos, gerado em grandes volumes. Apresenta potencial para ser utilizado na elaboração de bebidas em função do seu alto valor nutricional, sendo rico em minerais e fonte de proteínas biologicamente valiosas. Os leites fermentados são bem aceitos pelos consumidores em função de suas propriedades probióticas conferidas pelas bactérias ácido-láticas, proporcionando benefícios à saúde. Neste contexto, o aproveitamento do soro ácido líquido para a preparação de leites fermentados demonstra ser uma alternativa interessante de uso do subproduto. Além de enriquecer funcional e nutricionalmente a bebida, evita a necessidade de outros processos de conversão e possibilita a redução dos problemas ambientais relacionados ao seu descarte. Este trabalho tem por objetivo caracterizar fisico-quimicamente o soro ácido proveniente da fabricação de queijo tipo Petit Suisse e utilizá-lo como ingrediente para elaboração de leite fermentado. O soro ácido foi coletado fresco em uma indústria de laticínios e submetido às análises de pH, acidez, teor de lactose, gordura, proteínas, umidade e extrato seco durante o período de novembro de 2011 a novembro de 2012. O leite fermentado foi elaborado utilizando-se o soro ácido em substituição à água da fórmula original e com redução do tempo de fermentação da base de 60 para 25 horas. Todos os ingredientes foram pasteurizados e misturados com auxílio de um mixer, sendo a bebida caracterizada em relação a pH, brix, acidez, proteínas, lactose e açúcares redutores. O produto foi submetido ao teste de comparação múltipla utilizando-se como padrão o produto comercial, seguido de um teste de aceitação e de intenção de compra. Os valores das avaliações físico-químicas do soro ácido foram tratados estatisticamente pelo Teste de Fisher, considerando-se a média e o desvio padrão. Os resultados do teste de comparação múltipla foram analisados por ANOVA. Os resultados das análises físicoquímicas do soro ácido demonstram que as médias são similares às encontradas por diversos autores, sendo observados valores um pouco maiores para proteínas. Foram encontradas diferenças significativas no soro obtido no verão e no inverno para todos os parâmetros, exceto lactose. Os maiores teores de minerais, lactose e consequentemente de sólidos totais foram observados durante o outono. O teste de comparação múltipla evidenciou que o leite fermentado foi considerado por todos os provadores treinados como inferior ao padrão. O teste de aceitação resultou em 90 % de aceitação, com as médias de respostas entre os termos da escala hedônica “gostei muitíssimo” e “gostei moderadamente”. Na análise de intenção de compra constatou-se que 54 % dos consumidores que participaram da degustação certamente comprariam o produto e 38 % provavelmente comprariam. A utilização do soro ácido para elaboração de leite fermentado se mostrou tecnicamente viável, resultando em agregação de valor e redução do descarte desse subproduto bem como do consumo de água.

Encontrar formas de reutilizar os resíduos oriundos da produção de alimentos é uma necessidade cada vez mais presente nas indústrias tanto do ponto de vista ambiental como financeiro. Este trabalho teve por objetivo incorporar soro ácido na base láctea e no preparado de frutas de uma bebida láctea fermentada sabor morango e avaliar a interferência destas variáveis independentes nas características físico-químicas e reológicas do produto, bem como o efeito do tempo nestas respostas. O planejamento experimental seguiu o delineamento fatorial completo do tipo rotacional, com duas variáveis explanatórias. Os níveis de soro ácido variaram nas formulações, sendo na base láctea aplicadas menores dosagens (de 0 a 10%) devido a limitação por causa da precipitação da caseína em pH ácido no tratamento térmico. No preparado de frutas a dosagem de soro ácido variou de 0 a 30%. Os tratamentos (base láctea adicionada de preparado de frutas) foram acondicionados em potes de polipropileno e estocados a 8°C. Semanalmente foram feitas as análises físico-químicas de pH, acidez e sinerese além das análises reológicas. A análise dos dados foi feita pelo método de superfície de resposta e análise de regressão múltipla. Os tratamentos foram avaliados quanto a composição centesimal após 21 dias de estocagem pela análise dos teores de gordura, cinzas, proteínas e matéria sólida total, seguindo posteriormente com o cálculo de carboidratos e valor energético. A contagem de bactérias láticas foi feita por plaqueamento em MRS (Man Rogosa & Sharpe) Ágar após 21 dias de estocagem para garantir a contagem mínima exigida no tempo de vida útil final do produto. Todos os resultados obtidos nas análises de composição centesimal e microbiológicas se enquadraram nos limites da legislação vigente. De forma geral o percentual de soro ácido aplicado no preparado de frutas não influenciou os parâmetros físico-químicos avaliados, exceto para a viscosidade em que foi identificado efeito estatisticamente significativo indicando que o aumento nos níveis utilizados aumentam a viscosidade. A utilização do soro ácido na base láctea ocasionou aumento da acidez e diminuição da sinerese, estando os resultados de acidez em concordância com os dados da literatura para estudos semelhantes. O aumento do percentual de soro na base promoveu redução na viscosidade, afetando negativamente este parâmetro, porém, alternativas tecnológicas (uso de espessantes, pressão de homogeneização, temperatura no tratamento térmico, entre outras) podem suprir a perda de viscosidade apresentada. Todos os tratamentos apresentaram comportamento não-newtoniano e tixotrópico. / Finding ways to reuse waste from food production is an increasing necessity in this industry both for environmental and financial reasons. This study aimed to incorporate acid whey in milk-based and fruit prepared of a fermented dairy drink, strawberry flavor, and assess the effect of these independent variables on the physicochemical and rheological characteristics of the product as well as the effect of time on these responses. The experimental design followed a complete factorial design of the rotational type, with two explanatory variables. Acid whey levels ranged in the formulations being applied to dairy-based lower doses (from 0 to 10%) due to a limitation because of the precipitation of casein at acidic pH during heat treatment. In the fruit prepared the dosage of acid whey ranged from 0 to 30%. Treatments (milk added base of fruit prepared) were placed in polypropylene pots and stored at 8°C. Physicochemical analysis of pH, acidity and syneresis beyond rheological analyzes were conduced weekly. Data analysis was performed by the response surface method and multiple regression analysis. Treatments were nutritionally assessed after 21 days of storage by the analysis of the levels of fat, ash, protein and total solids, later following with the calculation of carbohydrates and energy value. The count of lactic acid bacteria was carried out by plating on MRS (Man Rogosa & Sharpe) Agar after 21 days of storage to ensure a minimum count time required in the final product shelf life. All results obtained in the analysis of chemical and microbiological composition fulfilled the bounds of current legislation. Overall the percentage of acid whey applied to the fruit prepared did not affect the physicochemical parameters evaluated, except for the viscosity in which was identified a statistically significant effect indicating that an increase in levels used, increase viscosity. The use of acid whey dairy based resulted in increased acidity and reduced syneresis, being acidity results in agreement with literature data for similar studies. The increase in the percentage of acid whey in the base promoted reduction in viscosity, adversely affecting this parameter, however, alternative technologies (use of thickeners, pressure homogenization, heat treatment temperature, etc.) can fill the given viscosity loss. All treatments showed non- Newtonian and thixotropic behavior.

The growth of the Greek-style yogurt market is causing many problems for dairy companies who are trying to handle the voluminous whey by-product. Acid whey, unlike sweet whey, has a low amount of protein and high amounts of lactic acid, calcium, and other minerals. Therefore, it has limited commercial value to the food industry and often requires additional processing for disposal. Lactic acid and calcium solutions have shown efficacy in increasing the tenderness of beef and other types of meat. The purpose of this project is to investigate the use of acid whey, with its high amounts of lactic acid and calcium, to tenderize beef (eye of round, IMP 171C) during marination. This study evaluated the effects of marination of utilizing acid whey in improving quality parameters of beef. 13 roasts (Top round steaks from USDA Select steers) were randomly assigned to one of six marination treatments: (1) calcium chloride, (2) lactic acid, (3) phosphate (4) acid whey (lot 1), (5) acid whey (lot 2), and (6) control. Steaks were marinated in vacuum pouches, aged for 48 hours, cooked to 70º C and evaluated by a consumer sensory panel and other quantitative tests (texture analyzer, colorimeter, collagen, cook loss, and pH). Marination with acid whey increased the tenderness and juiciness ratings without decreasing hedonic liking scores for the overall flavor or aftertaste of the beef samples.

The phenolic compounds as food components represent the largest group of secondary metabolites in plant foods. The phenolic compounds, e.g. chlorogenic acid (CQA), are susceptible to oxidation by enzymes specially, polyphenol oxidase (PPO) and at alkaline conditions. Both enzymatic and non-enzymatic oxidations occur in the presence of oxygen and produce quinone, which normally further react with other quinone to produce colored compounds (dimers), as well as is capable of undergoing a nucleophilic addition to proteins. The interactions of proteins with the phenolic compounds have received considerable attention in the recent years where, plant phenolic compounds have drawn increasing attention due to their antioxidant properties and their noticeable effects in the prevention of various oxidative stress associated diseases. Green coffee beans are one of the richest sources of chlorogenic acids. Therefore, a green coffee extract would provide an eligible food relevant source for phenolic compounds for modification of proteins. The interaction between 5-CQA and amino acid lysine showed decrease in both free CQA and amino acid groups and only a slight effect on the antioxidative capacity depending on the reaction time was found. Furthermore, this interaction showed a large number of intermediary substances of low intensities. The reaction of lysine with 5-CQA in a model system initially leads to formation of 3-CQA and 4-CQA (both are isomers of 5-CQA), oxidation giving rise to the formation of a dimer which subsequently forms an adduct with lysine to finally result in a benzacridine derivative as reported and confirmed with the aid of HPLC coupled with ESI-MSn. The benzacridine derivative containing a trihydroxy structural element, was found to be yellow, being very reactive with oxygen yielding semiquinone and quinone type of products with characteristic green colors. Finally, the optimal conditions for this interaction as assessed by both the loss of CQA and free amino groups of lysine can be given at pH 7 and 25°C, the interaction increasing with incubation time and depending also on the amount of tyrosinase present. Green coffee bean has a higher diversity and content of phenolics, where besides the CQA isomers and their esters, other conjugates like feruloylquinic acids were also identified, thus documenting differences in phenolic profiles for the two coffee types (Coffea arabica and Coffea robusta). Coffee proteins are modified by interactions with phenolic compounds during the extraction, where those from C. arabica are more susceptible to these interactions compared to C. robusta, and the polyphenol oxidase activity seems to be a crucial factor for the formation of these addition products. Moreover, In-gel digestion combined with MALDI-TOF-MS revealed that the most reactive and susceptible protein fractions to covalent reactions are the α-chains of the 11S storage protein. Thus, based on these results and those supplied by other research groups, a tentative list of possible adduct structures was derived. The diversity of the different CQA derivatives present in green coffee beans complicates the series of reactions occurring, providing a broad palette of reaction products. These interactions influence the properties of protein, where they exposed changes in the solubility and hydrophobicity of proteins compared to faba bean proteins (as control). Modification of milk whey protein products (primarily b-lactoglobulin) with coffee specific phenolics and commercial CQA under enzymatic and alkaline conditions seems to be affecting their chemical, structural and functional properties, where both modifications led to reduced free amino-,thiol groups and tryptophan content. We propose that the disulfide-thiol exchange in the C-terminus of b-lactoglobulin may be initiated by the redox conditions provided in the presence of CQA. The protein structure b-lactoglobulin thereupon becomes more disordered as simulated by molecular dynamic calculation. This unfolding process may additionally be supported by the reaction of the CQA at the proposed sites of modification of -amino groups of lysine (K77, K91, K138, K47) and the thiol group of cysteine (C121). These covalent modifications also decreased the solubility and hydrophobicity of b-lactoglobulin, moreover they provide modified protein samples with a high antioxidative power, thermally more stable as reflected by a higher Td, require less amount of energy to unfold and when emulsified with lutein esters, exhibit their higher stability against UV light. The MALDI-TOF and SDS-PAGE results revealed that proteins treated at alkaline conditions were more strongly modified than those treated under enzymatic conditions. Finally, the results showed a slight change in emulsifying properties of modified proteins. / Für die Verbesserung von Nahrungsmitteleigenschaften können Modifikationen an verschiedenen Inhaltsstoffen vorgenommen werden. Beispielsweise werden bereits Proteine miteinander verknüpft und bilden sogenannte „Crosslinks“ oder vernetzte Biomoleküle. Diese werden für die Herstellung fester, viskoelastischer Produkte, die zum Verdicken als auch zum Stabilisieren von Emulsionen oder Schäumen eingesetzt werden, genutzt. Da die Verbraucher sich Zunehmens mit gesundheitsfördernden Lebensmitteln befassen, ist das Einbringen von gesundheitsfördernden Inhaltsstoffen wie z.B. phenolische Verbindungen, immer mehr in den Fokus der Forschung gerückt. Demnach ist das wissenschaftliche Bestreben phenolische Verbindungen in die Vernetzung von Proteinen mit einzubeziehen und deren positive Wirkungen (antioxidativ) auszunutzen, vorteilhaft. Als Phenole werden Verbindungen bezeichnet, die eine oder mehrere Hydroxygruppen am Benzolring aufweisen. Phenole liegen in der Enolform vor, da diese, bedingt durch den Erhalt des aromatischen Benzolringes, energetisch begünstigt ist. Kaffeesäure ist eine Hydroxyzimtsäure und in Kaffeebohnen zu finden. Der am häufigsten anzutreffende Ester besteht aus Kaffee- und Chinasäure. Der einfachste Vertreter ist die Chlorogensäure (5-Caffeoylchinasäure, 5-CQA), die in vielen Pflanzenteilen enthalten ist. Chlorogensäure und ihre Derivate besitzen ebenfalls antioxidative Eigenschaften. Zusätzlich wirken sie auf Enzyme, die an entzündlichen- oder allergischen Reaktion teilnehmen, inhibierend. Während Verarbeitungs- und Lagerungsprozessen können phenolische Komponenten pflanzlicher Lebensmittel mit den Aminosäuren der Proteine in Lebensmitteln reagieren. Solche Reaktionen können die physikalisch-chemischen Eigenschaften von Proteinen verändern und deren ernährungsphysiologische Wertigkeit vermindern. Proteine weisen verschiedene reaktive Seitengruppen (Sulfhydryl-, Hydroxyl-, Aminogruppen) auf, mit denen sie über kovalente und nicht-kovalente Wechselwirkungen mit Phenolen Verbindungen eingehen können. Zu den nicht-kovalenten Verbindungen gehören u. a. Wasserstoffbrückenbindungen, hydrophobe Wechselwirkungen und Ionenbindungen. Phenole (z.B. Chlorogensäuren) können bei Anwesenheit von Sauerstoff enzymatisch bzw. nichtenzymatisch oxidiert werden. Die Reaktionsprodukte (Chinone) bilden anschließend mit reaktiven Thiol- bzw. Aminogruppen von Proteinen Addukte. Die Erfassung dieser verschiedenen Facetten von Interaktionen stellt somit die primäre Forschungsaufgabe im Rahmen dieser Arbeit. Die primäre Aufgabe der vorliegenden Arbeit besteht demzufolge in der Etablierung der Analysen- und der Charakterisierungsmöglichkeiten solcher Wechselwirkungen (Bindung) pflanzlicher Verbindungen bzw. deren Reaktionsprodukten mit Proteinen u.a. über massenspektrometrische Methoden. Da die Wechselwirkung mit Proteinen auch zu Veränderungen der Proteinstruktur führt, können deren funktionelle Eigenschaften auch verändert sein. Dies soll anhand der Messung von isolierten Proteinen die an der Wechselwirkung beteiligt sind, nachgewiesen werden. Anschließend sollen über Docking-Untersuchungen die entsprechenden Bindungsstellen näher charakterisiert werden. Durch die vorliegenden Ergebnisse wurden mögliche Reaktionen von phenolischen Verbindungen mit Proteinen, näher charakterisiert. Es wurde festgestellt, dass die Apfelsorte Braeburn über die höchste PPO- Enzymaktivität beim gleichzeitigen niedrigen CQA Gehalt im Vergleich zu den anderen untersuchten Sorten verfügt. Die PPO/Tyrosinase modulierte Reaktionen zwischen CQA und Lysine wurden in Abhängigkeit der vorherrschenden Bedingungen optimiert und die Reaktionsprodukte analysiert. In dem zweiten Teil wurden solche Reaktionsmöglichkeiten in den Grünen Kaffeebohnen lokalisierte und modelliert. Dazu wurden die sortenabhängige CQA-Zusammensetzung ermittelt und die möglichen Reaktionen mit den Hauptspeicherproteinen des Kaffees dargestellt. Im letzten Teil wurden dann diese Reaktionen mit Molkenproteinen simuliert und Einflüsse auf die Struktur und die funktionellen Eigenschaften erfasst. Die Ergebnisse belegen eine umfangreiche und sehr heterogene Adduktbildung mit den Aminoseitenketten des Lysins und Cysteins. Ein Katalog der unterschiedlichen Reaktionsprodukte wurde erstellt und am Protein modelliert. Die entsprechende Veränderung an die Proteinstruktur wurde experimentell belegt und der Einfluss wurde in den technofunktionelle Eigenschaften (wie die Löslichkeit, Emulgierbarkeit usw.) wiederspiegelt. Ein Anstieg des antioxidativen Potentials der Proteine wurde erreicht und diese so modifizierten Proteine wurden weiter zur Stabilisierung und Produktentwicklung getestet. Die ersten Ergebnisse eröffnen Nutzungsmöglichkeiten der modifizierten Proteine zur Verkapselung von bioaktiven Sekundären Pflanzenstoffen.

Encontrar formas de reutilizar os resíduos oriundos da produção de alimentos é uma necessidade cada vez mais presente nas indústrias tanto do ponto de vista ambiental como financeiro. Este trabalho teve por objetivo incorporar soro ácido na base láctea e no preparado de frutas de uma bebida láctea fermentada sabor morango e avaliar a interferência destas variáveis independentes nas características físico-químicas e reológicas do produto, bem como o efeito do tempo nestas respostas. O planejamento experimental seguiu o delineamento fatorial completo do tipo rotacional, com duas variáveis explanatórias. Os níveis de soro ácido variaram nas formulações, sendo na base láctea aplicadas menores dosagens (de 0 a 10%) devido a limitação por causa da precipitação da caseína em pH ácido no tratamento térmico. No preparado de frutas a dosagem de soro ácido variou de 0 a 30%. Os tratamentos (base láctea adicionada de preparado de frutas) foram acondicionados em potes de polipropileno e estocados a 8°C. Semanalmente foram feitas as análises físico-químicas de pH, acidez e sinerese além das análises reológicas. A análise dos dados foi feita pelo método de superfície de resposta e análise de regressão múltipla. Os tratamentos foram avaliados quanto a composição centesimal após 21 dias de estocagem pela análise dos teores de gordura, cinzas, proteínas e matéria sólida total, seguindo posteriormente com o cálculo de carboidratos e valor energético. A contagem de bactérias láticas foi feita por plaqueamento em MRS (Man Rogosa & Sharpe) Ágar após 21 dias de estocagem para garantir a contagem mínima exigida no tempo de vida útil final do produto. Todos os resultados obtidos nas análises de composição centesimal e microbiológicas se enquadraram nos limites da legislação vigente. De forma geral o percentual de soro ácido aplicado no preparado de frutas não influenciou os parâmetros físico-químicos avaliados, exceto para a viscosidade em que foi identificado efeito estatisticamente significativo indicando que o aumento nos níveis utilizados aumentam a viscosidade. A utilização do soro ácido na base láctea ocasionou aumento da acidez e diminuição da sinerese, estando os resultados de acidez em concordância com os dados da literatura para estudos semelhantes. O aumento do percentual de soro na base promoveu redução na viscosidade, afetando negativamente este parâmetro, porém, alternativas tecnológicas (uso de espessantes, pressão de homogeneização, temperatura no tratamento térmico, entre outras) podem suprir a perda de viscosidade apresentada. Todos os tratamentos apresentaram comportamento não-newtoniano e tixotrópico. / Finding ways to reuse waste from food production is an increasing necessity in this industry both for environmental and financial reasons. This study aimed to incorporate acid whey in milk-based and fruit prepared of a fermented dairy drink, strawberry flavor, and assess the effect of these independent variables on the physicochemical and rheological characteristics of the product as well as the effect of time on these responses. The experimental design followed a complete factorial design of the rotational type, with two explanatory variables. Acid whey levels ranged in the formulations being applied to dairy-based lower doses (from 0 to 10%) due to a limitation because of the precipitation of casein at acidic pH during heat treatment. In the fruit prepared the dosage of acid whey ranged from 0 to 30%. Treatments (milk added base of fruit prepared) were placed in polypropylene pots and stored at 8°C. Physicochemical analysis of pH, acidity and syneresis beyond rheological analyzes were conduced weekly. Data analysis was performed by the response surface method and multiple regression analysis. Treatments were nutritionally assessed after 21 days of storage by the analysis of the levels of fat, ash, protein and total solids, later following with the calculation of carbohydrates and energy value. The count of lactic acid bacteria was carried out by plating on MRS (Man Rogosa & Sharpe) Agar after 21 days of storage to ensure a minimum count time required in the final product shelf life. All results obtained in the analysis of chemical and microbiological composition fulfilled the bounds of current legislation. Overall the percentage of acid whey applied to the fruit prepared did not affect the physicochemical parameters evaluated, except for the viscosity in which was identified a statistically significant effect indicating that an increase in levels used, increase viscosity. The use of acid whey dairy based resulted in increased acidity and reduced syneresis, being acidity results in agreement with literature data for similar studies. The increase in the percentage of acid whey in the base promoted reduction in viscosity, adversely affecting this parameter, however, alternative technologies (use of thickeners, pressure homogenization, heat treatment temperature, etc.) can fill the given viscosity loss. All treatments showed non- Newtonian and thixotropic behavior.