Anaerobic membrane bioreactors may provide a sustainable technological solution for digestion of waste activated sludge due to their capacity to achieve substantial volatile solids (VS) destruction and positive energy balances with reduced digester volumes. However, membrane integrated anaerobic systems may have limitations that are imposed by membrane fouling and a decrease in biomass activity due to possible exposure of biomass to high shear conditions. This study characterised bioprocess and membrane performance under varying conditions, identified foulant type and origin and mechanism of fouling, and developed fouling control strategies by using low cross flow velocity and pressure anaerobic membrane systems. The study employed a pilot scale anaerobic digester integrated with negative and neutral tubular membranes; pilot and bench scale control digesters supported with bench scale filtration unit parametric studies. The membranes were polyvinylidene difluoride based with an average pore size of 0.02 micron and were operated at a constant cross flow velocity of 1 ms-1 and constant trans-membrane pressure of 30 kPa. Four operating conditions consisting of different combinations of HRT and SRT were evaluated. By integrating membranes into the digesters it was possible to simultaneously enhance digestion and increase throughput of the digesters without affecting its performance. The anaerobic membrane digester showed 48-49% volatile solids destruction at 30 days SRT under conventional and higher loadings of 1.2±0.4 and 2.1±0.6 kg COD m-3day-1. This was a 100% increase in performance compared to a control digester subjected to higher loading. This result was supported by the associated specific methane generation. The control digesters operated at a relatively higher SRT showed comparable VS destruction and gas generation to the anaerobic membrane running at a similar SRT. However the extra gas generated didn’t compensate heat required to maintain larger volume of the digester. In case of anaerobic membrane digesters due to the high rate feeding, increase biogas production and co-thickening, the energy balance increased by 144 and 200% under conventional and higher loading conditions respectively. Characterization of membrane performance showed that the average sustainable flux was 23.2±0.4 and 14.8±0.4 LMH during HRT-SRTs of 15-30 and 7-15 days respectively. The critical fluxes were in the range of 30-40, 16-17 and 20-22 LM-2H-1 during HRT-SRTs of 15-30, 7-30 and 7-15 days respectively. The decline in membrane performance at a higher loading was associated with the formation of cake layers on the membrane surface that led to reversible fouling. The additional decline in performance at extended SRT was attributed to irreversible fouling. The colloidal fraction of the sludge showed an overall higher fouling propensity during the long term pilot studies and short term filtration tests. The suspended solids fraction of the sludge showed a positive impact at concentration below 15 g/L but resulted in a decrease of membrane performance at higher concentrations. Further studies of foulant origin through a series of microscopic, membrane cleaning and sludge characterization studies showed that the colloidal proteins, soluble carbohydrates and inorganic materials such as iron, calcium and sulfur and their interaction to have a significant impact on membrane fouling. To control anaerobic membrane fouling by the digested sludge, integration of membrane relaxation techniques in the filtration cycle were found effective. By incorporating a unique relaxation technique to tubular membranes, it was possible to increase the sustainable flux to 29.2±1.8 and 34.5±2.5 LM-2H-1 for neutral and negative membranes during 15-30 HRT-SRT process condition. Addition of cationic polymers and sequential mechanical-citric acid membrane cleaning, that targeted both reversible and irreversible fouling was also found effective.

Deflocculation is a common upset event in biological wastewater treatment plants and causes significant problems in biosolids discharge and environmental management. However, fundamental understanding of deflocculation is limited. The overall objective of this work was to explore the fundamentals for deflocculation under transients of short-term low dissolved oxygen (DO). The investigation was carried out in a sequence of batch and continuous experiments on activated sludge, followed by batch experiments on E. coli suspensions. Both batch and continuous experiments on activated sludge demonstrated deflocculation of bioflocs under the transients of low DO (< 0.5 mg/L). Under the short-term low DO (in hours), turbidity increased by 20 times in the batch system and by 1-2 times in the continuous system, concentrations of suspended solids increased by 1-2 times, number of small particles (< 12.5 mm) increased by 2 times, more soluble EPS (proteins and humic substances) were released into supernatant or treated effluents, the removal efficiency of organic compounds was reduced by 50-70%. A 40% of increase in bulk K+ but a 30% of decrease in bulk Ca2+ under the DO limitation were observed in the batch experiments. There were significant increases in bulk K+ and decreases in bulk Ca2+ in the continuous experiments. Reversible changes were observed within 24 hours once the DO stress was removed. Floc strength of the remaining bioflocs after deflocculation increased. Deflocculation under the short-term low DO was consistent with an erosion process. The addition of selected chemicals (i.e., Ca2+, tetraethylammonium chloride, glibenclamide, and valinomycin) did not prevent deflocculation under the short-term low DO. It is proposed that a DO stress causes an efflux of cellular K+ but an influx of extracellular Ca2+, resulting in a decreasing ratio of Ca2+/K+ in extracellular solution and thereby causing deflocculation. The E. coli tests supported that increasing bulk K+ under the DO limit was due to the release of cellular K+ and was a stress response to the DO limitation.

Deflocculation is a common upset event in biological wastewater treatment plants and causes significant problems in biosolids discharge and environmental management. However, fundamental understanding of deflocculation is limited. The overall objective of this work was to explore the fundamentals for deflocculation under transients of short-term low dissolved oxygen (DO). The investigation was carried out in a sequence of batch and continuous experiments on activated sludge, followed by batch experiments on E. coli suspensions. Both batch and continuous experiments on activated sludge demonstrated deflocculation of bioflocs under the transients of low DO (< 0.5 mg/L). Under the short-term low DO (in hours), turbidity increased by 20 times in the batch system and by 1-2 times in the continuous system, concentrations of suspended solids increased by 1-2 times, number of small particles (< 12.5 mm) increased by 2 times, more soluble EPS (proteins and humic substances) were released into supernatant or treated effluents, the removal efficiency of organic compounds was reduced by 50-70%. A 40% of increase in bulk K+ but a 30% of decrease in bulk Ca2+ under the DO limitation were observed in the batch experiments. There were significant increases in bulk K+ and decreases in bulk Ca2+ in the continuous experiments. Reversible changes were observed within 24 hours once the DO stress was removed. Floc strength of the remaining bioflocs after deflocculation increased. Deflocculation under the short-term low DO was consistent with an erosion process. The addition of selected chemicals (i.e., Ca2+, tetraethylammonium chloride, glibenclamide, and valinomycin) did not prevent deflocculation under the short-term low DO. It is proposed that a DO stress causes an efflux of cellular K+ but an influx of extracellular Ca2+, resulting in a decreasing ratio of Ca2+/K+ in extracellular solution and thereby causing deflocculation. The E. coli tests supported that increasing bulk K+ under the DO limit was due to the release of cellular K+ and was a stress response to the DO limitation.

Filamentous bulking is a problem that has long plagued activated sludge (AS) wastewater treatment plants (WWTPs). Much research has looked at its prevention and control but there is still no solution. The sludge microbiological community is very complex and there are many factors that can affect bulking. Clay addition in scaled-down activated sludge systems was investigated at concentrations of 0.4, 2.0 and 5.0 g/L along with sequencing batch reactor (SBR) parameters when run with a synthetic wastewater (SWW). The 5.0g/L concentration exhibited positive results on settling in the form of modified SVI but appeared to cause no reduction in filament length. These preliminary investigations indicate that clay may help improve sludge settling but make no difference in the abundance of filamentous microorganisms. The SBRs exhibited trends in regards to running systems with a synthetic wastewater. A loss of volatile suspended solids (VSS), coupled with increase in sludge volume index (SVI), suggested a link between lack of non-VSS and settling ability. This has implications in the importance of non-VSS such as grit or clay in research performed using SWWs.

Xenobiotic Organic Compounds (XOCs) are present in wastewater and wastewater-impacted environmental systems. Pharmaceuticals and personal care products are a broad and varied category of chemicals that are included among these compounds. Although, these compounds have been detected at low levels in surface water, concerns that these compounds may have an impact on human health and aquatic life, have led to increased interest in how XOCs are removed during wastewater treatment. Recognizing specific mechanisms in recent literature and simulating those mechanisms responsible for the removal of XOCs is the main objective of this study. Conventional models, such as the popular activated sludge models (ASM1, ASM2, etc), do not sufficiently address the removal processes; therefore, a fate model is created to provide a means of predicting and simulating removal mechanisms along with experimental analyses. GPS-X is a multi-purpose modeling tool for the simulation of municipal and industrial wastewater treatment plants. This software package includes conventional models as built-in libraries, which can be used as bases on which new models can be created. In this thesis, the removal mechanisms of XOCs are recognized and investigated; a new library for GPS-X is also created to include XOCs. As a first step the uncalibrated fate model, which includes all mechanisms of interest with their process rates and state variables, is developed using in GPS-X software. A modified ASM1 (Mantis model) is used as a basis for developing the fate model. Since only a group of mechanisms is responsible for the removal of each compound the mechanisms are categorized in three different case studies as the next step. Thus, one submodel is associated with each case study. The model developer toolbar in GPS-X software is used to develop the model for these case studies. The first case study involves the removal of antibiotics, such as Sulfamethoxazole. The removal mechanisms used in this case are biodegradation, sorption, and parent compound formation, with co-metabolism and competitive inhibition effects being inserted into the structure of the model. Secondly, the removal of nonylphenol ethoxylates (NPEOs) occurs through abiotic oxidative cleavage, hydrolysis, and biodegradation. The third case study includes removal mechanisms of biodegradation and sorption for neutral and ionized compounds. In the calibration process, model parameters are tuned such that the model can best simulate the experimental data using optimization methods. A common error criterion is Sum of Squared Errors (SSE) between the simulated results and the measured data. By minimizing SSE, optimal values of parameters of interest can be estimated. In each case study different data sets were used for the validation process. To validate the calibrated model, simulated results are compared against experimental data in each case study. The experimental data set used in the validation process is different from that used for calibrating the model, which means the validation process data set was obtained from the different literature. By looking at the validation results, it is concluded that the proposed model successfully simulates removal of XOCs. Since the operating parameters of wastewater treatment plants, such as Solids Retention Time (SRT) and Hydraulic Retention Time (HRT) are crucial for the fate of XOC???s, a sensitivity analysis is carried out to investigate the effect of those parameters. Moreover, the pH effect is studied because it relates to the ionized XOCs. Sensitivity analysis results show that the fate model is more sensitive to model parameters i.e. biodegradation rate constant (kb) than the operational parameters, i.e. SRT and HRT. Furthermore, the responses showed sensitivity to pH, whereby acidic conditions provide a better environment for removing neutral forms and alkaline conditions were suitable for removing ionized forms, according to the ionized compound fate model.

Based on a diverse consortia of research completed within the last 15 years, it has been found that Pharmaceutical Compounds (PCs) are present in detectable levels within a variety of environmental matrices, including tap water. This is largely attributed to anthropogenic activities as humans are the majority consumer of PCs. As a result, the primary method of disposal is via wastewater pathways resulting from human excretion of ingested PCs. Based on past research into PC fate via the wastewater treatment process, only limited biotic and abiotic transformations are achieved – most PC’s are detected in the effluents of WWTP’s. This suggests that improving the removal of PCs during the wastewater treatment process provides a promising strategy for limiting the conveyance of PCs to the environment. Historically, studies regarding PC fate in WWTPs have predominantly focused on the activated sludge process. However, fixed film (biofilm) wastewater treatment technologies continue to gain popularity at full scale wastewater treatment facilities. The limited studies which investigated fixed film wastewater treatment processes have reported that improved transformation efficiencies were observed relative to activated sludge systems. Based on these previous studies, it was postulated that the more diverse bacterial consortium present within the Integrated Fixed Film Activated Sludge (IFAS) process, a novel treatment process which has recently gained popularity in North America, may lead to improved transformation efficiencies (“removals”) of these very complex compounds. Only one previous study which investigated the transformation efficiencies of the IFAS process compared to a control was found. It was therefore considered that an additional investigation into the IFAS process warrants further investigation. Four IFAS Sequencing Batch Biofilm Reactors (SBBRs) and four control Sequencing Batch Reactors (SBRs) were operated with varied experimental conditions in a 22 factorial design to investigate whether an observable difference in the level of PC transformations would result via the IFAS process when compared to a control. Experimental conditions were characterized by varying the operating Solids Retention Time (SRT) and mixed liquor temperature. For all other operational parameters, best efforts were made to ensure both reactors were operated under equivalent conditions. This permitted a true assessment of the effects of the inclusion of IFAS media. Reactors were investigated through three phases of sampling, under which the performance of the reactors was investigated through the measurement of the following parameters: • Conventional parameters (tCOD, sCOD, TAN, NO3-N) within the initial and final samples; • Operational parameters (MLSS, MLVSS, ESS); and • The transformation efficiencies achieved for 5 PC (Carbamazepine, Sulfamethoxazole, Trimethoprim, Atenolol and Acetaminophen). During all three phases of PC sampling, the pilot reactors were found to have been performing as anticipated with respect to conventional contaminant removals. Organic removals were found to be statistically similar between the IFAS and control reactors across all four experimental conditions. Full nitrification was observed for all reactors with the exception of the control SBR operated under the low SRT, low temperature condition. The IFAS SBBRs were found to demonstrate improved nitrification kinetics when compared to their respective controls operated under the same experimental conditions. This was believed to be related to the more diverse bacterial consortia present as a result of the IFAS biofilms. All reactors were generally believed to be operating at steady state and were within an acceptable range of the target operating conditions. Due to complications associated with the analysis of samples, only CBZ, TRIM, ATEN and ACE could be successfully quantitated. CBZ was found to not have been transformed to any appreciable level across all conditions investigated through either the IFAS SBBRs or control SBRs. ACE was transformed at efficiencies greater than 99% under all conditions and in both IFAS and control reactors and therefore no comparison could be made. TRIM and ATEN demonstrated improved transformation efficiencies under all conditions within the IFAS reactors. The presence of IFAS media, SRT and temperature were all found to be statistically significant effects through ANOVA using a confidence limit of 95%.

District heating has a long standing tradition in Sweden and today it is the most common way of producing and transporting heat. A District heating system (DH system) is divided into three parts: a production facility, distribution network (DH network) and one more heat stations. The heat produced in the facilities is distributed to the customers via a heat transfer medium, usually water (DH water), in piping networks that make up the DH network. The heat is transferred to the customers via the heat exchanger at which point they can use it as heated tap water or for heating purposes. The DH networks are often constructed in steel as it is cheap and a relatively resistant material. However it has the disadvantages of corrosion and expansions when it is exposed high temperatures which lead to damages in the DH network resulting in loss of the DH water, this is an unavoidable occurrence in any DH network. This results in addition of pollutants by leakages into the DH network or with the water that is used to compensate for the losses. The pollutants cause further corrosion, leading to metal contamination, and more damages on the DH network meaning there is a continuous degradation. Therefore various treatments are used to clean and ascertain an acceptable chemical environment in the DH systems. These treatments are effective but not at a level which is required so many chemicals are used to enhance the treatment of the water. Some of these are known to be toxic to humans and water ecosystems. As leakages are abundant and often end up in the WWTPs of the concerned municipality, which often have troubles with disturbances of the biological treatment, it was decided that an assessment of the toxic effects that DH water pose on activated sludge was to be investigated. This was done by testing water from two DH networks, Växjö and Kalmar, on the same activated sludge obtained from Tegelviken WWTP in Kalmar. A respirometric bioassay approach established by the Organization for Economic Co-operation and Development (OECD), OECD standard 209; OECD Guidelines for the Testing of Chemicals was used with changes made to exposure and measuring time as this decrease the risk of misinterpretation of the results. A dilution series using different concentrations (6.25%, 25% and 100%) of DH water was tested and compered to a blank control samples containing only activated sludge. Assessment of toxicity on total oxidation, oxidation carbon and oxidation of nitrogen was made. To get some idea of what might cause toxic effect samples of the waters was sent to outside laboratories for analyses of metals. The result from the bioassay and metal analysis was used to formulate risk factors associated with a DH water spill and exposure to WWTPs. It was found that both DH waters have a significant inhibition on nitrification in WWTPs. The DH water from Kalmar exhibited similar toxicity dynamics, roughly 20% inhibition, despite large differences in concentration. The DH water from Växjö showed a negative correlation between an increase in concentration of DH water and toxicity, 74% for the lowest concentration and 11% for the highest. The metal analysis concluded that there was no abundance of metal contamination which led to the inference that toxicity is probably caused by the chemicals used for treatment. This poses a great risk for the Baltic Ocean as many WWTPs release their treated water directly into water courses with a short detention time before reaching the sea.

From June to November 2007, a microscopic analysis was conducted on the activated sludge from eleven selected wastewater treatment plants (WWTP's) belonging to the City of Cape Town. The primary objective was the identification of the dominant and secondary filamentous organisms. Other important criteria included were the floe character, diversity, filament index (Fl) and identification of the protozoan and metazoan communities. The operational data determined from routine analyses of the sludge, influent and effluent were used to assess the relationship of the filamentous population to wastewater characteristics and to compare this with previous findings. Fl values of >3 and dissolved sludge volume indices (DSVI's) of >150 were chosen as representing the possibility of bulking conditions being present. The five most prevalent dominant filaments were Type 0092, Type 1851, actinomycetes, Microthrix parvicella and Type 021N, being present in 74%, 31%, 22%, 17% and 14% of samples respectively. Type 0092 did not appear to be associated with bulking in any of the WWTP's, although it was often incidentally present as a co-dominant species when bulking conditions existed. All three WWTP's with the Modified Ludzack-Ettinger configuration harboured Type 1851 as the major dominant species, irrespective of whether the plants treated domestic or industrial effluent. Conditions suggestive of bulking were present in two of these WWTP's. Contrary to expectations, Type 1851 was often found as a dominant species where domestic waste was the primary influent. Type 021N and actinomycetes were strongly implicated when bulking occurred. The overgrowth of these filaments appeared to be related to factors such as nutrient deficiency (Type 021N) or the presence of large amounts of low molecular weight substances in the influent. Microthrix parvicella did not cause major bulking problems. There was a strong association between low levels of nitrates/nitrites in the clarifier supernatant and good phosphorous removal, irrespective of the configuration of the WWTP. The converse was also true. / Thesis ((M. Environmental Science))--North-West University, Potchefstroom Campus, 2009.

From June to November 2007, a microscopic analysis was conducted on the activated sludge from eleven selected wastewater treatment plants (WWTP's) belonging to the City of Cape Town. The primary objective was the identification of the dominant and secondary filamentous organisms. Other important criteria included were the floe character, diversity, filament index (Fl) and identification of the protozoan and metazoan communities. The operational data determined from routine analyses of the sludge, influent and effluent were used to assess the relationship of the filamentous population to wastewater characteristics and to compare this with previous findings. Fl values of >3 and dissolved sludge volume indices (DSVI's) of >150 were chosen as representing the possibility of bulking conditions being present. The five most prevalent dominant filaments were Type 0092, Type 1851, actinomycetes, Microthrix parvicella and Type 021N, being present in 74%, 31%, 22%, 17% and 14% of samples respectively. Type 0092 did not appear to be associated with bulking in any of the WWTP's, although it was often incidentally present as a co-dominant species when bulking conditions existed. All three WWTP's with the Modified Ludzack-Ettinger configuration harboured Type 1851 as the major dominant species, irrespective of whether the plants treated domestic or industrial effluent. Conditions suggestive of bulking were present in two of these WWTP's. Contrary to expectations, Type 1851 was often found as a dominant species where domestic waste was the primary influent. Type 021N and actinomycetes were strongly implicated when bulking occurred. The overgrowth of these filaments appeared to be related to factors such as nutrient deficiency (Type 021N) or the presence of large amounts of low molecular weight substances in the influent. Microthrix parvicella did not cause major bulking problems. There was a strong association between low levels of nitrates/nitrites in the clarifier supernatant and good phosphorous removal, irrespective of the configuration of the WWTP. The converse was also true. / Thesis ((M. Environmental Science))--North-West University, Potchefstroom Campus, 2009.

This thesis was the report of the research done on the near-critical water gasification (NCWG) as an application for activated sludge treatment. The research started with the use of model compounds and binary mixtures of these compounds as feeds for the NCWG. High gasification yields were obtained using a commercial catalyst (Raney nickel), and it was found that interactions between model compounds in the binary mixtures resulted in lowering the gasification efficiencies. The research then shifted to the use of actual activated sludge samples and the search for novel catalysts for that application. Almost 70% of the sludge was gasified in the presence of the high amounts of Raney nickel. Hydrogen was the main product in the gas phase. However, Raney nickel lost half its activity after only 8 minutes of exposure to supercritical water. For some model compounds, novel catalysts formulated in our laboratories had better activities than the commercial ones. This was not the case for the NCWG of activated sludge.