<p>A wave digital filter is derived from an analog filter, which is realized as classical doubly resistively terminated reactancefilters. Perfectly designed wave digital filters express good dynamic signal range, low roundoff noise and excellent stabilitycharacteristics with respect to nonlinearity which are produced due to finite wordlength effects. Wave digital filters inheritthe sensitivity properties from analog filters, therefore, coefficients values can be selected to favorable values.Wave digital filters, derived from ladder filters, have low coefficient sensitivity in the passband and stopband. These WDFsare very complicated and are non-modular. The lattice wave digital filters are modular and are not complex. However, theyhave very high sensitivity in the stopband and thus require large coefficient wordlengths. The number of coefficients equalsthe filter order which have to be odd.This thesis discusses the wave digital filter structures that are modular because they are designed by cascading the first-orderand second-order sections. These WDFs can be pipelined. They also exhibit all the above mentioned favorable properties.Similar to lattice WDFs, these structures are restricted to symmetrical and antisymmetrical transfer functions. The synthesisof these structures is based on the factorization of the scattering matrix of lossless two-ports.In this thesis work, lowpass wave digital filters based on circulator-tree structure are designed with different orders startingfrom 3 and going upto 13. In parallel to these circulator-tree wave digital filters, the simple digital filters are also designedwith the same specification. The results of the two filters are compared with each other. It is observed that impulse responseand attenuation response of the two kind of filters perfectly match. Therefore, it is can be concluded that circulator-tree WDFupto Nth order can be synthesized. The implementation examples of two filter with order 3 and order 7 is presented in thisdocumentation for ready reference. It has also been shown that the order of sections does not affect the transfer function ofthe filter. Noise has been introduced and adaptor sections are penetrated. From the results it is concluded that the order of theadaptor sections does not matter and also that the noise does not affect the other adaptors sections, it only propagates throughother adaptors sections.</p>

A wave digital filter is derived from an analog filter, which is realized as classical doubly resistively terminated reactancefilters. Perfectly designed wave digital filters express good dynamic signal range, low roundoff noise and excellent stabilitycharacteristics with respect to nonlinearity which are produced due to finite wordlength effects. Wave digital filters inheritthe sensitivity properties from analog filters, therefore, coefficients values can be selected to favorable values.Wave digital filters, derived from ladder filters, have low coefficient sensitivity in the passband and stopband. These WDFsare very complicated and are non-modular. The lattice wave digital filters are modular and are not complex. However, theyhave very high sensitivity in the stopband and thus require large coefficient wordlengths. The number of coefficients equalsthe filter order which have to be odd.This thesis discusses the wave digital filter structures that are modular because they are designed by cascading the first-orderand second-order sections. These WDFs can be pipelined. They also exhibit all the above mentioned favorable properties.Similar to lattice WDFs, these structures are restricted to symmetrical and antisymmetrical transfer functions. The synthesisof these structures is based on the factorization of the scattering matrix of lossless two-ports.In this thesis work, lowpass wave digital filters based on circulator-tree structure are designed with different orders startingfrom 3 and going upto 13. In parallel to these circulator-tree wave digital filters, the simple digital filters are also designedwith the same specification. The results of the two filters are compared with each other. It is observed that impulse responseand attenuation response of the two kind of filters perfectly match. Therefore, it is can be concluded that circulator-tree WDFupto Nth order can be synthesized. The implementation examples of two filter with order 3 and order 7 is presented in thisdocumentation for ready reference. It has also been shown that the order of sections does not affect the transfer function ofthe filter. Noise has been introduced and adaptor sections are penetrated. From the results it is concluded that the order of theadaptor sections does not matter and also that the noise does not affect the other adaptors sections, it only propagates throughother adaptors sections.

Cheung, Hei Nga. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 63-72). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstracts also in Chinese.

Hydatidiform mole (HM) is an abnormal human pregnancy characterized by cystic degeneration of chorionic villi and absence of embryo. It has been correctly proposed that deregulation of imprinted genes, expressed in a parent-of-origin specific pattern, leads to this pathology due to the fact that biparental and androgenetic HMs are indistinguishable at the phenotypic level. To determine the extent of the abnormal DNA methylation in two biparental moles from a family with a mutation in NALP7, we assessed long interspersed nuclear elements (LINEs), inactive X-linked genes and three tumour suppressor genes and demonstrated their normal levels of methylation. Since the identification of the NALP7 as the causative gene of recurrent HMs, the role of inflammation and immunity has come into light as a possible cause of this disease. Due to the known role of NALP7 in cytokine processing, we addressed the ability of the patients' peripheral blood mononuclear cells (PBMCs) to secrete cytokines in response to stimulation with various antigenic molecules. We found a reduced level of IL-1beta and TNF-alpha secretion by the patients' PBMCs suggesting that abnormal processing of several cytokines may underlie this disease.

Dendritic cells (DC) are critical to an immune response by functioning as sensors of foreign antigen and presenting antigen to direct T cell responses. Under the influence of Flt3 ligand this heterogeneous group of cells originate from hematopoietic stem cells (HSCs) in the bone marrow and takes up residence in lymphoid organs, skin as well as mucosal surfaces where they are most likely to encounter pathogens. GM-CSF is another cytokine involved in DC development that is specifically involved in the maintenance of skin resident DC. Together Flt3L and GM-CSF as well as their respective receptors, Flt3 and GM-CSFR, are the most important factors identified to date for maintenance of DC homeostasis. The Src-like adaptor proteins, SLAP and SLAP-2, are negative regulators of antigen receptor signaling. SLAP and SLAP2 contain SH3 and SH2 protein interaction modules that facilitate interaction with proline-rich sequences and phosphotyrosine motifs, respectively. Through a unique C-terminal region, SLAP and SLAP2 also interact with the E3 ubiquitin ligase, c-Cbl. SLAP and SLAP2 enhance c-Cbl-mediated ubiquitylation and down-regulation of antigen receptors by binding both activated receptors and c-Cbl. SLAP and SLAP2 have also been shown to function as negative regulators of receptor tyrosine kinases (RTKs) and our group has shown that SLAP and SLAP2 bind to and inhibit the colony-stimulating factor-1 receptor (CSF-1R). In the work presented here, we identify a novel role for SLAP in regulation of the GM-CSF receptor of bone marrow (BM)-derived DC. We show that potentiated GM-CSF signaling, in the absence of SLAP and SLAP2, impairs BM-DC maturation such that these cells express minimal MHCII, secrete low amounts of IL-12 and are functionally impaired in their ability to stimulate T cell responses. SLAP and SLAP2 deficiency also has an effect on Flt3L-derived BM-DC development. For example, SLAP/SLAP2-/- BM-DC numbers are reduced in the presence of Flt3L as compared to wild-type BM-DC. To investigate the mechanism of reduced DC numbers, we examined splenic DC and found that DC numbers were similar in wild-type and SLAP/SLAP2-/- mice. In fact, SLAP/SLAP2-/- mice had proportionally more CD8α+ splenic DC in vivo than wild-type mice. Thus there may be cytokines affected by SLAP/SLAP2-deficiency in our cultures that are either dispensable or compensated for in vivo DC development. The work presented in this thesis has implications for the role of SLAP and SLAP2 in immune response to infections by the regulation of GM-CSFR and Flt3 in maintaining dendritic cell homeostasis.

Dendritic cells (DC) are critical to an immune response by functioning as sensors of foreign antigen and presenting antigen to direct T cell responses. Under the influence of Flt3 ligand this heterogeneous group of cells originate from hematopoietic stem cells (HSCs) in the bone marrow and takes up residence in lymphoid organs, skin as well as mucosal surfaces where they are most likely to encounter pathogens. GM-CSF is another cytokine involved in DC development that is specifically involved in the maintenance of skin resident DC. Together Flt3L and GM-CSF as well as their respective receptors, Flt3 and GM-CSFR, are the most important factors identified to date for maintenance of DC homeostasis. The Src-like adaptor proteins, SLAP and SLAP-2, are negative regulators of antigen receptor signaling. SLAP and SLAP2 contain SH3 and SH2 protein interaction modules that facilitate interaction with proline-rich sequences and phosphotyrosine motifs, respectively. Through a unique C-terminal region, SLAP and SLAP2 also interact with the E3 ubiquitin ligase, c-Cbl. SLAP and SLAP2 enhance c-Cbl-mediated ubiquitylation and down-regulation of antigen receptors by binding both activated receptors and c-Cbl. SLAP and SLAP2 have also been shown to function as negative regulators of receptor tyrosine kinases (RTKs) and our group has shown that SLAP and SLAP2 bind to and inhibit the colony-stimulating factor-1 receptor (CSF-1R). In the work presented here, we identify a novel role for SLAP in regulation of the GM-CSF receptor of bone marrow (BM)-derived DC. We show that potentiated GM-CSF signaling, in the absence of SLAP and SLAP2, impairs BM-DC maturation such that these cells express minimal MHCII, secrete low amounts of IL-12 and are functionally impaired in their ability to stimulate T cell responses. SLAP and SLAP2 deficiency also has an effect on Flt3L-derived BM-DC development. For example, SLAP/SLAP2-/- BM-DC numbers are reduced in the presence of Flt3L as compared to wild-type BM-DC. To investigate the mechanism of reduced DC numbers, we examined splenic DC and found that DC numbers were similar in wild-type and SLAP/SLAP2-/- mice. In fact, SLAP/SLAP2-/- mice had proportionally more CD8α+ splenic DC in vivo than wild-type mice. Thus there may be cytokines affected by SLAP/SLAP2-deficiency in our cultures that are either dispensable or compensated for in vivo DC development. The work presented in this thesis has implications for the role of SLAP and SLAP2 in immune response to infections by the regulation of GM-CSFR and Flt3 in maintaining dendritic cell homeostasis.

Dissertation (Ph.D.).--University of Texas Graduate School of Biomedical Sciences at San Antonio, 2007. / Vita. Includes bibliographical references.

Thesis (Ph.D.). / Written for the Division of Experimental Medicine, Dept. of Medicine. Title from title page of PDF (viewed 2008/03/12). Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 85-100).

Dissertation (Ph.D.).--University of Texas Graduate School of Biomedical Sciences at San Antonio, 2007. / Vita. Includes bibliographical references.