The search for a low cost and effective technique to control and remove volatile organic chemicals (VOCs) has gathered a great attention from the adsorption process field. Advances in manufacturing technology have enabled the creation of activated carbon monoliths (ACM) as promising substitute for traditional packed beds of granular adsorbent materials. The research described in this thesis comprises an extensive experimental study of a single component adsorption process onto square and hexagonal channel Novacarb™ ACM supplied by MAST Carbon Technology Ltd. ACM characterisation methods such as nitrogen and solvent adsorption isotherms, electron microscopy, thermo-gravimetric analysis and thermal dynamic characterization have been used. High BET surface area, high total pore volume and high total solvent mass uptakes have been found. ACM were tested by obtaining column breakthrough curves mainly using dichloromethane and acetone as the adsorbates at the bench-scale. The adsorption dynamics of the ACMs studied were also compared with those of extrudates manufactured by the same process as the ACMs. The influence of humidity on the adsorption process has been studied at the bench-scale. Finally, the adsorption system was scaled-up to about 60cm length monoliths in order to study both adsorption and electrical regeneration taking advantage of the particular electrical properties held by the Novacarb™ ACM. It has been found that ACMs are able to adsorb high levels of VOCs, up to 40% by weight of DCM, good behaviour under humidity conditions and low pressure drop. In contrast, kinetics of ACMs have been found to be somewhat inferior to those of equivalent packed beds, although the ACM performance can be improved by reducing the wall thickness. Adsorption of DCM at the pilot-scale has demonstrated that the Novacarb™ ACM could easily be used in a cyclic thermal swing adsorption process with a half cycle time of less than one hour.

This work investigated the combined technology of adsorption on hydrophobic molecular sieves (zeolites) and direct UV (254 nm) photolysis for removing estrone (E1) from water. The target compound estrone belongs to the group of endocrine-disruptor compounds (EDCs) that are raising more and more concern due to increasing evidence of their adverse estrogenic effects on aquatic organisms and humans. Current wastewater treatment processes remove less than 80% of estrone on average. However, because of its strong biological potency, small amounts are still able to exert adverse estrogenic effects on aquatic systems. Consequently, advanced treatment technologies have been investigated in the hope of reaching higher removal efficiency. Adsorption of estrogens on hydrophobic zeolites in this work is a potential new alternative. Based on the hydrophobic nature of estrogens including E1, two types of zeolites, dealuminated Y (DAY) and silicalite-1, and a type of granular activated carbon Centaur® activated carbon (GAC) were evaluated for adsorption capacity. The results demonstrated that DAY is the best adsorbent for E1 in that 99% E1 can be removed by DAY. Silicalite-1 was the least effective. Moreover, adsorption of E1 to DAY is much faster. Estrone reached adsorption equilibrium in 4 hours on DAY versus 8 days for GAC. The adsorption data of DAY for E1 were fit to the Freundlich and Langmuir equations and the maximum adsorption capacity is estimated as 74 mg E1/g DAY. Direct UV photolysis of E1 in solution was also evaluated. Short-wave UV (ë = 254 nm) degraded E1 in solution much more effectively than long-wave UV-light (ë = 365 nm). No significant increase in degradation of E1 in UV photolysis was found with the addition of hydrogen peroxide. The regeneration of E1-contaminated DAY was investigated by a series of adsorption/direct UV (ë = 254nm) irradiation cycles. No significant deterioration of adsorption capacity of DAY was observed over nine adsorption/regeneration cycles.

Artificial lipid membranes have been used in biophysical studies as well defined models of biological membranes. In the present work we studied adsorption of ions to artificial lipid membranes, composed of phosphatidylcholine (PC) and phosphatidylserine (PS), and to biological membrane from sarcoplasmic reticulum (SR). The studies of ion adsorption and electrokinetic characterization of membranes were done by means of microelectrophoresis of PC/PS liposomes and SR vesicles. The ions of interest were positively charged potassium (K), calcium (Ca), tetraphenylarsonium (TPAs), tetraphenylphosphonium (TPP) and negatively charged pentachlorophenol (PCP). Electrophoretic mobility of PC/PS liposomes and SR vesicles has been measured as a function of pH, ionic strength and the concentration of adsorbing ions. From the data we have determined the isoelectric point and the density of electric charge of the SR membrane. We have shown that the mobility of PC/PS liposomes and SR vesicles can be understood in terms of electrostatic screening of membrane surface charge by the diffuse double layer of counterions and by ion adsorption. The experimental results, with the exception of Ca and SR membranes, have been found consistent with the adsorption model based on Langmuir adsorption isotherm and Grahame's equation relating the membrane electric potential and the membrane surface charge. The adsorption of ions to membranes have been characterized by the ion association constant and the membrane surface area of adsorption site. These quantities have been obtained from the fit of the adsorption model to the electrophoretic mobility data and the results are reported in the thesis. The following two findings are most interesting and important. First, the adsorption of K and lipophilic ions TPAs, TPP, and PCP to both the SR membrane and the artificial PC/PS membrane can be well characterized by the the adsorption model. The theoretical predictions of the adsorption model agree with the experimental results for a single (although different for PC/PS and SR membranes) set of ion adsorption constant and adsorption site area. This indicates a similarity between the biomembrane and the artificial lipid membrane. In contrast, such similarity was not found for calcium. Adsorption properties of SR membrane and artificial lipid membrane for Ca were found to be very different. Whereas the adsorption of Ca on PC/PS membranes could be described by one set of adsorption sites, the results for the SR vesicles indicate the presence of more than two types of Ca adsorption sites in the SR membrane. This finding is of physiological significance since the SR membrane regulates calcium transport in the muscle cell. Second, the adsorption affinity of SR membrane to all ions has been found to be significantly smaller compared to that of the lipid bilayer. This indicates that membrane proteins in the SR membrane reduce ion adsorption. This effect cannot be due to electrostatic interactions because the artificial lipid membranes had similar surface charge density as the biological membrane and the reduced adsorption was observed for both the positively charged TPAs and TPP as well as for the negatively charged PCP.

No description available.

"April, 2002" Includes bibliographical references and Publications list (leaves 22-30) V. 1. nos.1-50 -- v. 2. nos. 51-105 "Selected research publications submitted to the University of Adelaide for the degree of Doctor of Science."

"April, 2002" / Includes bibliographical references and Publications list (leaves 22-30) / 2 v. (various paging) : / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / "Selected research publications submitted to the University of Adelaide for the degree of Doctor of Science." / Thesis (D.Sc.)--University of Adelaide, Dept. of Environmental Biology, 2003

University of Technology, Sydney. / Wastewater contains a matrix of organic and inorganic substances both in dissolved form and in suspension. Most of the biodegradable substances are removed in primary and secondary treatment processes. However, the conventional wastewater treatment processes cannot remove a number of synthetic and refractive organic substances. These substances can cause tremendous problem in the sewage treatment processes and in the water body where the effluent from the sewage plant is discharged. These substances produce odour, colour, and require a large quantity of disinfectant dose before the wastewater can be discharged into a water body. They can also significantly deplete the dissolved oxygen level of the water receiving body thus putting all the aquatic life in danger. The effluent from the sewage treatment plant therefore, needs to be passed through further treatment process, which is called advanced sewage treatment process. The advanced treatment processes consist of many treatment options. Depending upon the characteristics of the sewage and the level of treatment required, one has to select an appropriate treatment technology. Physico-chemical processes such as coagulation-flocculation and filtration, adsorption, and membrane application are some of the most viable treatment processes that can remove the organic substances to the desirable level. In this study, adsorption, biosorption or biofiltration, and adsorption-membrane hybrid systems were investigated for the removal of organics (in terms of total organic carbon (TOC)) from a low strength synthetic wastewater and a biologically treated secondary effluent from a sewage treatment plant, Sydney. Adsorption experiments were conducted on low strength synthetic wastewater and the biologically treated sewage effluent using granular activated carbon (GAC) and powder activated carbon (PAC). The synthetic wastewater was prepared using three organic substances (glucose, peptone and yeast extract) and seven inorganic chemicals (MnS04, CaCI2, NaHC03, NaCl, MgS04·7H20, KH2P04 , and NH2·NH2·H2S04). The biologically treated sewage effluent was collected from the St. Marys sewage treatment plant, Sydney. Detailed experimental studies on adsorption equilibrium, batch kinetics and fixed bed were carried out, and the experimental results were predicted using suitable mathematical models. The adsorption equilibrium was analysed with different initial organic concentration of the synthetic wastewater. The experimental results were then predicted using association theory (AT), characterization theory (CT), and the Freundlich isotherm. The experimental results showed unfavourable type of isotherm curve, and hence, the normal favourable isotherm equations such as Langmuir, Freundlich or Sipps isotherms were not very successful in describing the adsorption equilibrium results. The AT and the CT were better in predicting the adsorption equilibrium results than the commonly used Freundlich isotherm. In this process, the adsorption equilibrium (isotherm) parameters were determined using a multi variable, non-linear regression, Nelder-Mead method by optimising an object function defined as the mean percent deviation between experimental and calculated equilibrium adsorption amounts. The isotherm parameters were found to be dependent on the initial organic concentration. Hence, it is important to estimate the isotherm parameters covering a wide range of organIc concentration. Further, the adsorption equilibrium studies of the individual organic compounds indicated that the overall effects of the inorganic substances were unfavourable for the adsorption of organics in the wastewater. The organics of the synthetic wastewater were found to undergo biodegradation after 8 hours. Thus, the effect of the background substances in the wastewater, and the biodegradation effect are another important aspects that need to be considered while evaluating the effectiveness of the adsorption process for organic removal from the wastewater. It is equally important to study the adsorption behaviour with time (i.e. adsorption kinetics). Adsorption kinetics of the organics in the wastewater was determined using linear driving force approximation (LDFA) model. Basically, the LDFA is a simplified expression of intraparticle diffusion of adsorbate into adsorbent particles. In this model, it is assumed that the uptake rate of adsorbate by an adsorbent particle is linearly proportional to the driving force developed due to the difference between the surface concentration and the average adsorbed phase concentration of the adsorbate. The main reason for using the LDFA method was the use of index (or lumped) parameter, total organic carbon (TOC), to express the total organic contents of the wastewater. The film mass transfer coefficient (kf) was found to be dependent on the experimental conditions such as mixing intensity, the adsorbent dose and the initial organic concentrations. The film mass transfer coefficient (kf) to the adsorbent increased when the mixing intensity and the adsorbent dose were increased. However, the kf value decreased with the increase in the initial organic concentration of the solution. The adsorption isotherm parameters obtained from the association theory (AT) and the characterization theory (CT), were utilized to fit the experimental results using LDFA model. The isotherm parameters obtained from both the theories were found equally effective in predicting the experimental results. The overall effect of the dissolved inorganic compounds in the synthetic wastewater solution was observed to enhance the mass transfer rate to the GAC particle. The average value of the overall mass transfer rate was in the order of 10-6 mls. The application of adsorption system in practice is usually carried out in the fixed bed adsorption mode. The adsorbent (usually GAC) is packed in a column and the target pollutants are passed through either end to be adsorbed by the adsorbent. In this study, the fixed bed adsorption study was carried out in acrylic columns in the laboratory. The GAC bed depth, organic concentration of the feed solution, and the filtration velocity through the GAC bed were varied to evaluate the effectiveness of the fixed bed adsorption system. The experiments were carried out with both the biologically treated sewage effluent and the synthetic wastewater. The experimental results were predicted using the dynamic adsorption model. The film mass transfer coefficient (kf) was obtained by fitting the fixed bed experimental data. The kf increased when filtration rate was increased, but it decreased with the increase in the organic concentration of the feed solution. As expected, the value of kf remained constant with the increase in GAC bed depth. The effect of axial dispersion coefficient was negligible, as the GAC bed depth and the size of the GAC particles used in this study were shallow and small respectively. The average value of the overall mass transfer rate in the fixed bed study was also in the order of 10-6 mls but slightly less than that obtained in batch kinetics study. The fixed bed system with attached microorganisms on the surface of the adsorbent (fixed bed medium) is referred to as a biofilter, where the organics are adsorbed (biosorption) and biodegraded by the microorganisms. The fixed bed adsorption experimentations were conducted for a longer duration to investigate the biological activity on the granular activated carbon (GAC). The experimental results showed the growth of microorganisms on the surface of GAC particles. In other words, the adsorption system turned into biosorption or biofiltration system after few weeks of operation. The adsorption capacity of the GAC particles slowly exhausted with the growth of microorganisms with time. The overall organic removal efficiency of the system was however, not impaired by the growth of microorganisms. The organics were removed by the processes of biosorption and subsequent biodegradation. The biomass growth rate was found to fluctuate with time in pattern. Despite the fluctuation in the biomass, the TOC removal efficiency of the biofiltration system was consistent at 55 % for 77 days of continuous operation. Moreover, the daily backwashing provided at 30 % bed expansion to avoid filter clogging did not have adverse effect on the TOC removal efficiency of the biofilter. The organic removal efficiency of the biofilter changed when the filtration rate was altered from that in which the biofilter was acclimatized~ however the organic removal pattern remained consistent with time. This result suggests that the biofilter should be operated in the same filtration velocity at which it is acclimatized to attain maximum efficiency of the filter. A practical mathematical model was developed incorporating both adsorption and biodegradation of organics. The organic removal efficiency of the biofilter was successfully predicted using kinetics data obtained from the previous studies. The model was sensitive to the biofilm thickness and decay constant. The adsorption-membrane hybrid system is emerging as a cost-effective membrane process for the organic removal. In this system, the organics are adsorbed on the adsorbent and the organic laden adsorbents are removed by the membrane separation process. In this study, the adsorption-membrane hybrid system was evaluated using submerged hollow fibre (pore size 0.1 ~m), and the external loop crossflow rnicrofiItration. Powdered activated carbon (PAC) was used to reduce the direct organic loading onto the membrane surface. The main function of membrane in these studies was to remove the organic laden PAC particles. The submerged PAC-Membrane hybrid system was found effective in removing dissolved organic substances both from the synthetic wastewater and the biologically treated effluent of a sewage treatment plant. The system has potential for its long-term application in the treatment of wastewater without the need of frequent membrane cleaning. This preliminary study showed that the PAC-membrane hybrid system could be used for a long time effectively (over 47 days). At the initial stage of operation, the organic removal was mainly due to adsorption by PAC, but during the long-term application of the system, the adsorption capacity of the PAC was exhausted gradually, and the microbial communities developed on the PAC, in the suspension of the reactor, and on the membrane surface actively participated in the biodegradation of the organics. An empirical mathematical model was developed for the submerged hollow fibre membrane hybrid system. The model predicted the organic removal efficiency of the system satisfactorily. A new term, membrane correlation coefficient (MCC) was introduced in the model to account for the adsorption of organics onto membrane surface. The MCC and the filtration rate (flux) were found to be the main model parameters that controlled the quality of the effluent from the system. Greater the value of MCC, better was the organic removal efficiency of the system. The MCC value was found to increase with the increase in the PAC dose to the system. Since only the short-term experiments were conducted in this study, the biological degradation of the organics was not included in the model. It is necessary to incorporate the biological degradation part in the model to predict the long-term efficiency of the system. The external loop cross-flow microfiltration system with prior PAC addition was also tested using the synthetic wastewater. This study showed that the use of PAC helped not only in the organic removal but also in the enhancement of the filtration flux. The use of PAC was instrumental in increasing the operational life the membrane hybrid system by reducing the organic fouling on the membrane. The conventional pressure filtration models, cake filtration model (CFM) and standard blocking model (SBM) were used to successfully predict the experimental results. Since CFM was more effective in predicting the volume of the permeate flux from the hybrid system, one could infer that the fouling mechanism of the membrane was mainly due to the formation of cake layer on the membrane surface. However, the experimental conditions used in the hybrid system were not so favourable for removing the organics from the synthetic wastewater. The organic removal efficiency of the PAC-membrane hybrid system was only 250/0 for the PAC dose of 150 mg/L. The organic removal efficiency of the system depends mainly on the characteristics of the adsorbent and the influent wastewater solution, and the adsorbent dose. This study shows that activated carbon can effectively be used in different operational modes and in different treatment processes to remove organics from the wastewater, and to produce effluent of high quality that can be reused for many purposes.

In this study the enzymatic activity of adsorbed Thermomonospora fusca E��� and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential effect on enzyme structure and activity at an interface. Enzymatic activity was measured after partial elution of the adsorbed layer with both protein-free buffer and the surfactant, dodecyltrimethylammonium bromide. For E��� at high concentration (0.5 mg/ml), adsorbed enzyme activity decreased about 20% in increasing adsorption time from 0.25 h to 24 h. At low concentration (0.001 mg/ml), adsorbed enzyme activity decreased by one order of magnitude during a 24 h period. CBHI layers lost activity only after a sufficiently long contact time with the surface, and this effect was not strongly dependent on enzyme concentrations in solution. These findings were explained with reference to structural changes undergone by adsorbed enzyme as a function of time and available interfacial area. / Graduation date: 2000

Graduation date: 1999

Graduation date: 1999