The thesis deals with aerodynamic characteristics identification from flight measurement. The topic is part of flight mechanic – handling qualities. The first theoretic part consists of three identification methods description: Error equation method, Output error method and Filter error method. Mathematical model of an airplane is defined and restricted to the motion with 3 degree of freedom. There is also introduced simulation of flight measurement for identification software validation. Practical part is focused on experiment preparation, execution and evaluation. The airplane VUT 700 Specto had been chosen to carry out flight tests. The airplane was modified to the new electric powered VUT 700e Specto after first measurement flights with combustion engine. Data record from on-board measurement unit was completed by telemetric data from autopilot and remote control system. Flight tests were carried out in stabilised mode of autopilot in symmetric flight. The results were confronted with analytical analysis results and DATCOM+ software parameter estimation.