Gorse, Ulex europaeus, is a serious weed of agriculture and forestry in New Zealand. The mite Tetranychus lintearius feeds on gorse in Europe and was released in New Zealand during autumn 1989 as a potential biological control agent. The aim of my laboratory study was to increase available knowledge on its development and aggregation. As the quality of gorse declined as a food resource (as indicated by chlorophyll content) a significant decline in egg production, and an increase in development time of mites was found. Mean egg production at 15℃ ranged from 37.8 to 45.2 eggs/female over 10 days in the experimental treatments and development time from 39.2 to 43.3 days. Density of adult female mites (1, 2, 5, 10, 15, 20/spine) also had a significant effect on the number of eggs produced/female over ten days (mean daily egg production/female, 0.78-0.88). Mean development time from egg to adult decreased from 40.6 to 39 days as density increased from 1 to 50 mites/spine. The resident New Zealand strain of T lintearius, imported originally from Cornwall, is now widely established but has not established successfully on the west coast of the South Island or in the north of the North Island. Four new strains recently introduced from coastal Portugal and Spain, areas climatically similar to these New Zealand regions, may be more successful. Thus, although egg production and survival of all strains were significantly reduced when subjected to daily periods of simulated rainfall in laboratory experiments, those of the newly introduced strains were least affected. Laboratory experiments with the resident New Zealand and Cambados strains also showed that individuals aggregated actively at 10-20℃, but the latter aggregated more strongly at these temperatures. This strong aggregation behaviour may increase survival in warm, wet climates and suggests that Cambados strain mites could become established and so increase gorse control in Westland and Northland.

No description available.

This paper considers alternative methods to estimate econometric models based on bilateral data when only aggregate information on the dependent variable is available. Such methods can be used to obtain an indication of the sign and magnitude of bilateral model parameters and, more importantly, to decompose aggregate into bilateral data, which can then be used as proxy variables in further empirical analysis. We perform a Monte Carlo study and carry out a simple real world application using intra-EU trade and capital flows, showing that the methods considered work reasonably well and are worthwhile being considered in the absence of bilateral data. (authors' abstract) / Series: Department of Economics Working Paper Series

Proteins and peptides can convert from their native form into insoluble highly ordered fibrillar aggregates, known as amyloid fibrils. The process of fibrillogenesis is implicated in the pathogenic mechanisms of many diseases and, although mature fibrils are well characterised by a plethora of biophysical techniques, the initiation and early steps remain, to date, ambiguous. Mass spectrometry can provide invaluable insights into these early events as it can identify the low populated and transient oligomeric species present in the lag phase by their mass to charge ratio. Recent evidence has shown that oligomers formed early in the aggregation process are cytotoxic and may additionally be central to the progression of diseases associated with amyloid fibril presence. The hybrid technique of ion mobility mass spectrometry can be employed to provide conformational details of monomeric and multimeric species present and elucidate the presence of oligomers which possess coincident mass to charge ratios. Molecular modelling, in conjunction with experimental results, can suggest probable monomeric and oligomeric structural arrangements. In this thesis three aggregating systems are investigated: amyloidogenic transthyretin fragment (105-115), insulin and two Aβ peptides. Initially amyloidogenic endecapeptide transthyretin (105-115) is studied as it has been widely utilised as a model system for investigating amyloid formation due to its small size. Secondly insulin, a key hormone in metabolic processes, is investigated as extensive research has been carried out into its aggregation into amyloid fibrils. The formation of insulin amyloid fibrils rarely occurs in vivo; however localised amyloidosis at the site of injection and the aggregation of pharmaceutical insulin stocks present problems. Thirdly the aggregation of A β peptides Aβ (1-40) and Aβ (1-42) and their interactions with an aggregation inhibitor, RI-OR2, are characterised. A (1-42), although less commonly produced in vivo, is more cytotoxic and has a faster aggregation mechanism than Aβ (1-40). Both Aβ peptides are implicated in the aetiology of Alzheimer’s disease whilst RI-OR2 has been reported to prevent the production of high molecular weight oligomers, with particular suppression of Aβ (1-42) aggregation.

peripheral arterial disease (PAD), a limiting factor in the success of percutaneous transluminal angioplasty (PTA) is the development of restenosis secondary to vascular smooth muscle cell (SMC) proliferation. The aim of this study was to determine the effect of combination antiplatelet therapy on the ability of plasma, from patients undergoing PTA, to stimulate SMCs in vitro. We aimed to investigate the effect of combination treatment on levels of circulating adhesion molecules and factors which mediate SMC proliferation in experimental models. We also sought to demonstrate any association between changes in measured markers and the development of restenosis or vascular events. Methods Fifty patients were randomised to receive clopidogrel or placebo, for thirty days, in addition to their daily 75mg aspirin. To measure proliferative capacity, diluted plasma was incubated with 24h-growth-arrested rat vascular SMCs, and Extracellular-regulated-kinase (ERK)1/2 activation was analysed by Western blotting at baseline, 1-hour pre-PTA, and at 1-hour, 24-hours and 30-days post-PTA. Plasma platelet-derived growth factor (PDGF-BB), soluble (s)E-selectin, sICAM-1 (intracellular adhesion molecule-1) and von Willebrand factor (vWF) were measured by ELISA (Enzyme-linked immunosorbent assay), at the same time-points. Platelet activation was measured by flow cytometry of ADP-stimulated platelet fibrinogen binding at baseline and 1-hour post-PTA. Patients’ notes and all investigations were reviewed for 2 years post-PTA to record restenosis or vascular events. Results Samples were available for all 50 patients at baseline, 1-hour pre-PTA and 1-hour post-PTA timepoints. In this cohort ERK1/2 activation was significantly increased post-PTA in both the aspirin/clopidogrel and aspirin/placebo groups. Those who developed a symptomatic restenosis had a significantly higher level of SMC activation at the 1-hour post-PTA time-point. There was a statistically significant decrease in PDGF-BB, and increase in vWF, following loading with clopidogrel. sICAM-1 levels significantly decreased in the aspirin/placebo group following PTA. ADP-stimulated platelet fibrinogen binding was significantly inhibited by clopidogrel therapy post-PTA. Conclusions This is the first study to show in-vitro ERK1/2 activation (a marker of SMC proliferation) increases post-PTA. Patients developing a symptomatic restenosis had a significantly higher level of SMC activation at the 1-hour post-PTA time-point. Clopidogrel therapy had no significant effect on ERK1/2 activation, although it did reduce PDGF-BB in the larger cohort of patients. Further work is required to evaluate potential therapeutic treatments which may reduce peripheral PTA-induced smooth muscle cell activation.

<p>This work presents an improved aggregation model that accounts for two types of particles and simulates the heterogeneous aggregation between these particles. By accounting for the sizes, concentrations, and affinities of the nano- and background particles, the model can evaluate e.g. how the nanoparticles affect an existing distribution of natural aggregates or how quickly the nanoparticles will settle out of a given system, and can help determine which parameter to change in order to eliminate the nanoparticles from a system faster. The model could provide a powerful tool to evaluate the exposure of nanoparticles in environmental and engineered waters.</p> / Dissertation

The purpose of this research has been to explore the native monorhamnolipid (mRL) mixture produced by Pseudomonas aeruginosa ATCC 9027, as well as newly- synthesized monorhamnolipid diastereomers (R,R)-Rha-C10-C10, (R,S)-Rha-C10-C10, (S,S)-Rha-C10-C10 and (S,R)-Rha-C10-C10, to understand their aggregation and phase behavior in aqueous solution. This work is in response to the consideration that biosurfactants offer a "greener" alternative to conventional surfactants. Relationships between chemical structure of the diastereomers and surface activity were studied using surface tension measurements. It was found that the critical aggregation concentration (CAC) values are lower for deprotonated (R,S)-Rha-C10-C10 and higher for protonated (R,S)-Rha-C10-C10 compared to the other diastereomers or the native mRL mixture. Furthermore, the 1:1:1:1 diastereomeric mixture has the overall lowest cross-sectional area for deprotonated rhamnolipids. Dynamic light scattering (DLS) was used to study the hydrodynamic radii (Rh) of the mRL aggregates and the four diastereomers in aqueous solution. In all five surfactants studied, each observed at pH 8.0 and 4.0, three aggregate populations were observed. For all deprotonated rhamnolipids the micelle, at a hydrodynamic radius of ~2 nm, was found to be in the highest abundance where the two lower abundant other population take on a lamellar aggregate structure. Data to support this were collected using fluorescence probing techniques. For the surfactants in the protonated state, pH 4.0, it was found that all form primarily lamellar structures, also confirmed using fluorescence probing. The average aggregation numbers (Nagg) of the micelles for the deprotonated native mRL mixture and the four diastereomers were studied using steady-state and time-resolved fluorescence quenching measurements. Somewhat unexpectedly, the Nagg values were observed to be a strong function of the rhamnolipid concentration for all systems. At low concentrations, pre-micellar aggregates with aggregation numbers too small for micelles were observed. A critical concentration is identified at which a critical aggregation number is defined; this is proposed to be the smallest fully-formed micelle in solution with values of ~25-30 molecules/micelle for the native mRL mixture and all four diastereomers. Thus, the aggregation properties of the native mRL mixture and the four diastereomers are generally similar at this critical concentration. However, the increase in aggregation number above this critical point varies for all the surfactants, where the (R,R)-Rha-C10-C10 diastereomer has the greatest increase in monomers with concentration and the (S,S) and (R,S) diastereomers have the lowest. The increased observed aggregation number is consistent with the computational work showing increased growth with concentration for the deprotonated rhamnolipid micelles. Furthermore, these computations have confirmed the ability of protonated rhamnolipids to form lamellar structures in solution. Fluorescence probing work was also used to study rhamnolipid phase behavior as a function of varying solution conditions, such as ionic strength, solution pH, surfactant concentration, and temperature. Steady-state fluorescence methods are utilized to probe the surfactant microenvironment using the polarity-sensitive dyes prodan and laurdan. By dissolving the dye within the rhamnolipid aggregates and observing their solvatochromic behavior, all surfactants studied are known to form more micellar aggregates at high pH and low surfactant concentration, whereas elongation of the micelle is observed at low pH and moderate to high surfactant concentration.

Les régions qui gagnent, sont celles dans lesquelles existent, grâce à une décentralisation, notamment régionale, large et réelle, des centres de décision proches, des synergies fortes entre tous les acteurs de la vie sociale et économique, appuyés sur une identité reconnue. Au sein de la région PACA, l'agrégation financière territorialisée doit constituer un outil de transparence démocratique qui permet une dynamique à l'intérieur du territoire régional.L'agrégation se définit comme l'addition de certaines données financières de plusieurs niveaux de collectivités sur un territoire donné. Le champ et la nature des informations agrégées sont déterminés par les acteurs locaux en fonction de leurs besoins. Ils peuvent être globaux ou partiels, porter sur l'ensemble de l'action des collectivités ou sur une compétence déterminée.L'objectif de la démarche est de permettre à la région PACA d'avoir à sa disposition une restitution qui lui permette de connaître avec exactitude la situation financière des zones de son territoire. Actuellement, la représentation d'une région s'opère uniquement à travers les comptes du conseil régional. Certes, les régions opèrent des audits pour leur permettre de connaître la situation d'une zone sur son territoire, mais ces études sont isolées, incomplètes et peu représentatives de la réalité financière globale de leur territoire.Aussi, est-il nécessaire de mettre en œuvre un véritable outil qui permettrait d'avoir une vision complète et exacte des différentes zones territoriales dans la région.Les thèmes étudiés relèveront de l'économie spatiale, mais aussi des finances publiques territoriales et des systèmes de diffusion de l'information.Les travaux de recherche se sont étalés sur cinq années et présentent l'exercice 2008 comme une année de référence. Outre une présentation agrégée de la région, c'est une méthode scientifique qui est conçue pour y parvenir / The winning regions are those where a real decentralization, particularly regional, exists with local centres of decision and strong synergies between the different social and economic partners in a common identity.Within the PACA region, the territorial financial aggregate should constitute a dynamic tool for democratic transparency.The aggregate can be defined as the sum of certain financial data on several local community levels in a given territory. The content of the aggregate information is chosen by the local decision centres depending on their needs. This can be global or partial : concerning all or only certain community competences.Our aim is to make the exact financial situation of the different sectors of its territory avaible to the PACA region.At the present time, the information on the situation of any region can only be obtained from the accounts of the regional council. Indeep, the regions carry out audits to be informed of the situation of particular sectors, but these audits are isolated, incomplete and do not reflect the global financial reality of the territory. It is thus necessary to provide a tool capable of giving an exact and complete report on the different sectors. The themes to be studied will concern spatial economics, as well as territorial public finance and the diffusion of information. The research has taken place over five years and presents 2008 as the year of reference. In addition to an aggregate presentation of the region, this is a scientific method conceived to succeed

This study provides a comprehensive overview of changes in the autoregressive-moving- average model (ARMA) when applied to various functions. First, the necessary and sufficient condition for a weakly stationary stochastic process described by ARMA is given. Next, some particular transformations of ARMA processes are presented: first, non- correlated and generic sums of ARMA processes; next, products of independent and dependent Gaussian ARMA processes; and finally, time aggregations, namely, systematic sampling and temporal aggregations. Tables are included to clearly summarize special cases of particular transformations. Some of these cases are then demonstrated through concrete examples. In addition to theoretical results, extensive numerical simulation in statistical software R is also given, which systematically covers the obtained results.

Master of Science / Department of Physics / Jeremy Schmit / In this work, we present a model for the kinetics of amyloid fibril aggregation. In the model we mapped the process of Hydrogen bond (H-bond) formation and breakage to a random-walk. we captured the effect of side chains using position dependent H-bonds free energies which allows us to calculated the residence time for different binding alignments with the fibril. The residence time can be compared to the diffusion-limited attachment rate to give net aggregation stability. This stability increases exponentially with increasing number of bonds or binding energy in homopolymer chains, however for chains with patterned sequences, the residence time shows strong effects of the binding alignment. Using the residence time for uniform structures combined with estimate of the diffusion rate, we modeled and simulated the kinetics of amyloid aggregation. Results of the simulations gives the bond energies and concentrations required for the onset of growth of aggregates.