Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 5 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst. / Härtill 10 uppsatser och 1 appendix.

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Centenarian population studies are one of several approaches currently used to study the aging process and characterize successful aging. I have described a methodology permitting the simultaneous generation of RNA, DNA, protein, and plasma samples, as well as fixed peripheral blood mononuclear cells (PBMC) and frozen blood aliquots, from a single 10- to 30-ml sample of peripheral blood obtained from donors of any age, and showed that although extremely old individuals are somewhat anemic, it is possible to obtain enough biological material from their blood to conduct aging studies. / I investigated the possibility of immortalizing B-lymphocytes from extremely old individuals, using the Epstein-Barr virus (EBV), and found that although extremely old individuals (90+ years) possess low levels of circulating B-lymphocytes, it is possible to immortalize B cells present in less than one milliliter of their blood using EBV. / Using biological material obtained from blood samples of individuals of all ages by the method for blood sample processing I have described, I studied the mRNA expression of cell death (specifically caspase) genes in nonagenarians and centenarians, successful models of aging who have survived or avoided age-associated diseases, as well as in their younger counterparts, to determine whether apoptotic genes may be part of the genetic determinants of longevity. I found that a population of extremely old individuals (90+) shows a unique pattern of caspase mRNA expression, characterized by high levels of caspase-1 and -3, and low levels of caspase-8, mRNA, while slightly less aged individuals (70--89) are characterized by high levels of caspase-8 mRNA expression. Furthermore, I showed that these changes in caspase mRNA do not appear to result from age-related changes in PBMC composition, such as decreases in CD24. Therefore, I suggest that unique patterns of caspase mRNA result from the regulation of message abundance on a per cell basis, via a putative regulation of caspase genes at the transcription or RNA processing level, rather than age-associated changes in immune profiles.

by Leung Man-sing. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1986. / Includes bibliographical references.

Peng, Cheng. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 142-163). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

published_or_final_version / Physiology / Master / Master of Philosophy

BDNF is a neurotrophin that enhances neural health and is increased by exercise. PURPOSE: To compare moderate continuous (MCE) and high-intensity interval exercise (HIE) effects on serum BDNF levels, and examine the relationship between BDNF and lactate. METHODS: Seven males completed a VO2peak test and two protocols on separate days, (MCE) 28 min at 60% Workrate max (WRmax) and (HIE) 28 min of intervals at 90%WRmax (10- 1 min intervals separated by 2 min of rest). Serum BDNF and lactate were determined prior, during, and following both protocols. RESULTS: BDNF levels (pg/mL) increased from baseline during HIE and MCE (p<.05). The BDNF response to HIE correlated with lactate for area under the curve (AUC) (r=0.901; P<0.05). CONCLUSION: HIE is an effective alternative to MCE at increasing BDNF. Additionally, lactate may act as a measure of intensity or a mediator of the BDNF response to exercise. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection

Wu Yan. / "August 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 141-170). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

by Wong Ho Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 107-129). / Abstract --- p.i / List of Abbreviations --- p.ii / Contents --- p.iii / Chapter Chapter 1. --- Introduction / Chapter 1.1 --- Introduction of aging in central nervous system --- p.1 / Chapter 1.2 --- Introduction of hippocampus / Structure of the hippocampus --- p.4 / Function of hippocampus --- p.6 / Chapter 1.3 --- A literature review of aging in hippocampus / Cell loss in aging --- p.8 / Ultrastructural changes in aging --- p.9 / Changes in neurotransmitter system --- p.10 / Neuroglial change --- p.11 / Change in potentiation --- p.13 / Chapter 1.4 --- A literature survey of Nitric Oxide Synthase (NOS) / General introduction of Nitric Oxide Synthase --- p.15 / Introduction of nNOS --- p.15 / Introduction of iNOS --- p.16 / Introduction of eNOS --- p.17 / Similarities and differences among isoforms --- p.18 / Role of NO/NOS in neurotransmission --- p.19 / Role of NO in neurotoxicity --- p.23 / Chapter 1.5 --- Aim of study --- p.25 / Chapter Chapter 2. --- Change of nNOS in aging / Chapter 2.1 --- Purpose and approach --- p.27 / Chapter 2.2 --- Basic principle of the techniques / Basic principle of immunohistochemistry --- p.28 / Basic principle of RT-PCR --- p.28 / Chapter 2.3 --- Experimental procedure / nNOS immunohistochemistry --- p.32 / RT-PCR of nNOS --- p.34 / Chapter 2.4 --- Result / nNOS immunohistochemistry --- p.38 / RT-PCR of nNOS --- p.44 / Chapter Chapter 3. --- Expression of iNOS in aging / Chapter 3.1 --- Purpose and approach --- p.50 / Chapter 3.2 --- Experimental procedure / iNOS immunohistochemistry --- p.50 / RT-PCR analysis of iNOS --- p.51 / Chapter 3.3 --- Result / iNOS immunohistochemistry --- p.52 / RT-PCR analysis of iNOS --- p.56 / Chapter Chapter 4. --- Verification of the RT-PCR product of iNOS / Chapter 4.1 --- Purpose and approach --- p.58 / Chapter 4.2 --- Basic principle --- p.58 / Chapter 4.3 --- Experimental procedure / Elution of PCR product from PAGE gel --- p.60 / Restriction digestion of the eluted PCR product --- p.61 / Chapter 4.4 --- Result --- p.62 / Chapter Chapter 5. --- Identification of the iNOS-positive cells / Chapter 5.1 --- Purpose and approach --- p.64 / Chapter 5.2 --- Experimental procedure --- p.64 / Chapter 5.3 --- Result --- p.65 / Chapter Chapter 6. --- Quantitation of astrocyte in aging hippocampus / Chapter 6.1 --- Purpose and approach --- p.67 / Chapter 6.2 --- Experimental procedure --- p.68 / Chapter 6.3 --- Result --- p.69 / Chapter Chapter 7. --- Detection of apoptosis in aging / Chapter 7.1 --- Introduction of apoptosis --- p.74 / Chapter 7.2 --- Purpose and approach --- p.75 / Chapter 7.3 --- Basic principle --- p.76 / Chapter 7.4 --- Experimental procedure / TUNEL method --- p.77 / DNA gel electrophoresis --- p.78 / Chapter 7.5 --- Result / TUNEL method --- p.80 / DNA gel electrophoresis --- p.82 / Chapter Chapter 8. --- Discussion / Chapter 8.1 --- Pattern of neuronal NOS in aging / Localization of nNOS --- p.84 / Decrease in staining of nNOS in the hippocampus during aging --- p.87 / No change in nNOS mRNA level --- p.88 / nNOS in aging - past and present works --- p.89 / Implication of the result --- p.91 / Chapter 8.2 --- Increased iNOS expression in aging / Neurotoxicity of iNOS --- p.93 / Circumstances of iNOS expression --- p.95 / Discussion of the present study --- p.96 / Chapter 8.3 --- Detection of apoptosis in aging --- p.103 / Chapter Chapter 9. --- Conclusion --- p.106 / Biblography --- p.107 / Appendix --- p.130 / Acknowledgements --- p.134