Agro-climatic characterization of Lesotho for dryland maize farming was performed using temperature and rainfall indices in a GIS environment. The temperature and rainfall meteorological parameters were patched for missing data using the UK method for the maximum and minimum temperatures. Missing daily rainfall data was patched using the inverse distance method. Statistical evaluation of the patching methods showed good performance. The spatial distributions of different temperature variables and indices were mapped. Important meteorological parameters were the frost occurrence (first day, last day and duration) and monthly and seasonal heat units. The onset of frost is early (March) over the highland areas while the low- lying areas onset can be as late as June. The last day of frost over the low- lying areas is mostly in August and on the other hand, the highlands last day of frost is in November/December at some places. Rainfall interpolation was done using the kriging method of the geostatistical analyst. Important aspects mapped include monthly averages, seasonal amounts, annual amounts and number of days of high daily rainfall. Wet season (October to April) rainfall was high (>800mm) over the north to northeastern parts of the country while some areas over the east and southern parts received less than 500mm of seasonal rainfall. Climatic potential of maize under dryland farming in Lesotho was investigated using five climatic suitability indices namely: probability of receiving heat units of greater than 1500GDD, probability of a frost-free growing season, probability of seasonal rainfall of more than 500mm, probability of 15-day dry spells during December to February and the slope of an area. For each of the above parameters a coverage layer was prepared in GIS environment and the layers were overlaid to obtain the agroclimatic suitability map of maize in Lesotho. The districts of Butha Buthe, Leribe and Berea are shown to have areas which are highly favorable for maize cultivation under dryland farming while the unsuitable areas are mostly over the high- lying areas (Mokhotlong, Thaba Tseka and Qachaâs Nek) together with other parts of the southern lowlands.

Not available

The climate of the Canadian Prairies is semi-arid, thus moisture is a limiting factor in canola production. Previous research indicates that the alteration of the early season microclimate using tall wheat stubble from the previous year can create more favorable conditions for canola to emerge. The objective of this research was to test this approach over a broad range of climatic conditions across western Canada. In 2011 & 2012 field sites were established in Kenton, Swan Lake MB, Indian Head, Swift Current, SK and Lethbridge, Falher and Grimshaw AB. At each site, large replicated plots of tall stubble cut at 50 cm height were compared to large replicated plots of short stubble cut at 20 cm tall. The treatments were statistically analyzed to determine their effects on microclimates on canola during the spring seeding and fall harvest. This data provided an assessment of the possible benefits that stubble management could have on canola across a broad range of climatic conditions.

Maize based cropping systems

This project investigated the phenological development of canola through the 2009 growing season in the western Canadian prairies and quantified the effects of 624 weather parameters on nine canola quality parameters from 247 samples of Canada No. 1 canola. Predictive models were created to utilize as few of the most strongly correlated weather predictors as possible to explain a maximum amount of variation in each of the quality parameters. An intensive field study carried out at seven sites across Manitoba measured weather conditions and followed canola crop development from seeding through swathing, harvest or physiological maturity. These data were used to produce an index with six Physiological Day (P-Day) thresholds corresponding to specific growth stages. A comparison to the thresholds determined from a previous study suggested that current varieties require fewer heat units for early vegetative growth stages, more heat units during reproductive stages, and slightly greater P-Day accumulations to reach maturity. Canola samples from the field study were combined with western Canadian canola samples from collaborating companies and the 2008 and 2009 Canadian Grain Commission Harvest Surveys for quality analysis. The samples were analysed for oil content, protein content, oleic, linoleic, linolenic, and total saturated fatty acid contents and iodine value. Weather data from the intensive field study, collaborating companies, the Canadian Wheat Board and Environment Canada weather stations nearest each canola sample were compiled and arranged from the seeding to swathing date of each canola sample. These data were then used to calculate the accumulation of P-Day values from seeding until each of the six phenological growth stages. Partial Least Squares analysis was utilized to produce predictive models for each of the nine quality parameters. The results indicated that environmental parameters, especially temperature, had a significant impact on canola quality. The predictive models explained between 7 and 49% of the variation in individual quality parameters. The models for saturated fatty acids, glucosinolates and iodine value explained the highest amount of variation and the model for chlorophyll explained the least. Oil content was positively impacted by a longer duration of temperatures below 11-14oC throughout the reproductive stage, while protein was positively impacted by cool temperatures at early flowering and high temperatures throughout pod and seed development. Chlorophyll was strongly impacted by the moisture balance throughout early to mid reproductive stages and glucosinolates content was affected by conditions that impacted nutrient availability. Total saturated fatty acid content was positively impacted by cool temperatures throughout late vegetative and early reproductive stages. Moderate predictability of the individual fatty acid content models may have been indicative of either successful breeding of current canola varieties with relatively stable quality characteristics across a range of growing conditions or the complex interactions between oil content and the individual fatty acids measured. Producers looking to maximize canola quality and canola breeders interested in creating varieties more resistant to the specific weather conditions which impact canola quality could benefit from this study. Predictions of crop quality would also be an asset to those marketing Canadian canola as an export.

Crop models have great potential as research tools, for crop system management and policy analysis. One of the most promising future uses of crop models is in crop improvement. The limitation in the use of models for crop improvement has been the inability of crop models to predict variety differences. Currently, the CANEGRO model, a sugarcane crop model developed the South African Sugar Association Experiment Station (Inman-Bamber, 1991a) can only model the performance of the NC0376 variety. Experiments were undertaken in the South East Lowveld of Zimbabwe, which is a hot and dry environment where sugarcane is grown under irrigation, to examine the canopy growth and development of four commercial varieties, ZN6, ZN7, N14 and NC0376. The study aimed at determining variety differences in canopy (tillers and leaves) development, develop parameters that can be used to model variety differences and test the improved CANEGRO canopy model for its ability to predict variety differences in canopy growth and development. For the late season, the numbers of leaves and tillers produced by each variety were counted every fortnight throughout the crop cycle. The total leaf area of the varieties and the individual leaf area on a stalk were determined using a Delta-T leaf area meter every fortnight. The date of emergence of successive leaves on a stalk was recorded daily. The leaf angles of each variety were measured every fortnight. The amount of photosynthetically active radiation (PAR) intercepted by the varieties was measured using a SunScan Ceptometer. Tillering and tiller senescence rates, phyllochron intervals, extinction coefficients and base temperatures were determined for the growth and development processes of varieties ZN6, ZN7, N14 and NC0376. Tiller and leaf population development was varietal. Tillering and leaf emergence were highly correlated to thermal time while tiller and leaf senescence were less correlated to thermal time. The poor correlation of the senescence phases to thermal time could mean that tiller and leaf senescence was driven by other factors other than thermal time. PAR interception could be one of these factors. The data showed that PAR interception could be a trigger of tiller senescence. The study showed that the tiller and leaf population development could be approximated by two linear equations. Tillering will be the first linear phase and tiller senescence the second linear phase. The first linear phase is driven by thermal time. While the second linear phase is triggered by PAR interception, the major driving factors need to be determined. This study proposed the use of two linear equations to model tiller and leaf population development as opposed to the polynomial equations used in the current CANEGRO model. Polynomial equations assume the factors driving tillering and tiller senescence are the same. The green leaf numbers per plant showed that all varieties experienced a decline in green leaf numbers with crop age. Varieties NC0376 and ZN7 had the greatest decline in green leaf numbers per plant while varieties N14 and ZN6 had the least decline. Variety ZN7 had the highest number of green leaves per plant while NC0376 had the least. The tiller growth and development was divided into three phases: the exponential phase during the initiation of stalks, the first linear phase during a period of rapid stalk elongation and the second linear phase during sucrose accumulation and maturation. The first two phases of development were driven by thermal time while the sucrose accumulation was not. There were variety differences in tiller growth and development. There were variety differences in base temperature for the development of various components of the canopy. Internode formation occurred at lower air temperatures than stalk elongation and tillering while canopy heights were correlated with higher air temperatures. This implies that internode formation could occur under conditions unsuitable for stalk elongation and may explain the short internodes frequently observed in stalks exposed to winter during rapid stalk elongation. The basic requirements for physiological parameters are that they should be stable across different environments, have significant differences between varieties and have physiological meaning. The parameters studied were thermal time requirement for shoot emergence, leaf appearance, to reach peak tiller population and to start of stalk elongation; surface area of the youngest biggest leaf, leaf number of the youngest biggest leaf, PAR transmission at the start of tiller senescence, extinction coefficients, and peak and mature tiller population. The difference between varieties in thermal time to shoot emergence was least using a base temperature of 16 QC compared to using 10 QC and therefore 16 QC could be a more appropriate base temperature for shoot emergence. The accumulated soil temperatures were less variable than accumulated air temperature and could therefore be a more reliable driver of shoot emergence. However, the limitations in the use of soil temperature are that it is not a readily available measurement and that it is not easy to measure. The gradual increase in phyllochron intervals appeared to be a better method of predicting leaf appearance compared to using a broken stick model. The phyllochron gradient was proposed, as it is likely to be a more robust way of modelling leaf appearance. The varieties had different phyllochron gradients. Variety ZN7 had highest rate of leaf appearance and produced the highest number of leaves per stalk while NC0376 had the lowest rate and produced the least number of leaves. There were statistically significant differences between varieties (P = 0,05) in PAR transmission at the start of tiller senescence and a base temperature of 16 QC was best at determining accumulated thermal time to the start of tiller senescence. Varieties with higher peak tiller population had higher final tiller population, lower thermal time per tiller and a higher ratio of final to peak tiller population. There were differences between varieties in the youngest leaf number attaining maximum leaf area and the leaf area of the youngest biggest leaf. Variety Nl4 had the biggest leaves and NC0376 had the smallest. Variety Nl4 had the highest leaf area index (LAI) while ZN7 had the lowest. There were significant differences (P = 0,01) in PAR intercepted by the varieties but there were no significant differences in extinction coefficients. Extinction coefficients increased with crop age. The varieties had significantly different (P = 0,01) leaf angles and ellipsoidal leaf angle distribution parameters. The measurement of LAI using SunScan ceptometer provided a better estimate of extinction coefficients than LAI measured using Delta-T leaf area meter. Model evaluation showed that CANEGRO canopy model version 2 was improved compared to than version 1. The model (version 2) was accurate in predicting tiller heights and dead leaf numbers per stalk. It was fairly accurate in predicting green leaf numbers per plant, stalk population and intercepted PAR but was poor in predicting LA!. Version 2 has proved to be a substantial improvement over version 1 in predicting stalk population. Generally, the version 2 model overestimated tiller heights early and underestimated later, overestimated the tiller population and LAI after peak, underestimated green leaf numbers per stalk for varieties ZN6, ZN7 and N14 and overestimated dead leaf numbers per stalk and intercepted PAR. The version 2 model predicted a constant green leaf numbers per plant and LAI from peak to harvest while observed data showed that green leaf numbers per stalk and LAI decreased towards harvest. Version 2 model predicted the tiller population of NC0376 closely but underestimated tiller senescence in N14 and also underestimated final tiller population in varieties ZN6 and ZN7. Future model refinements may need to focus on the prediction of the sigmoid pattern of tiller heights. The model may need to be calibrated to predict the green leaf numbers per stalk accurately, which should possibly improve the prediction of LAI that in turn could improve the prediction of intercepted PAR. The improvement in the timing and rate of tiller senescence should improve the prediction of tiller population particularly in varieties ZN6, ZN7 and N14. The study showed that the broken stick method IS superior in explaining leaf and tiller population development compared to using polynomial equations. The development of variety parameters helped improve the prediction of variety differences in canopy growth and development. A major weakness of most crop models is modelling variety differences in canopy growth and development. The inability of crop models to predict variety differences has limited their use in plant breeding. This study has resulted in an improved version of CANEGRO version 1 that is an initial attempt at modelling variety differences of sugarcane. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003.

FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / Trata-se de um estudo da estimativa da evapotranspiraÃÃo de referÃncia com a equaÃÃo de Penman-Monteith/FAO, fazendo-se uso de diferentes metodologias nos cÃlculos mÃdios diÃrios da temperatura do ar, da umidade relativa do ar e do saldo de radiaÃÃo solar. Os dados utilizados compreenderam o perÃodo de janeiro a dezembro de 2002 e foram obtidos em uma estaÃÃo meteorolÃgica automatizada, localizada no municÃpio de Paraipaba, Estado do Cearà (latitude de 3Â26â S, longitude de 39Â08â W e altitude de 31m). Para o cÃlculo das mÃdias diÃrias de temperatura e da umidade relativa do ar utilizou-se a mÃdia aritmÃtica de 24 leituras horÃrias, as equaÃÃes propostas pela FAO, pelo Instituto Nacional de Meteorologia (INMET) e pelo ServiÃo de Meteorologia do Estado de SÃo Paulo (SMESP). No cÃlculo, para obtenÃÃo do saldo de radiaÃÃo solar diÃrio, utilizaram-se as equaÃÃes de radiaÃÃo global proposta pela FAO (mÃtodo 1), por Aguiar et al. (mÃtodo 2), por Glover & McCulloch (mÃtodo 3) e por Black (mÃtodo 4). Os resultados mostram que, para a estimativa da evapotranspiraÃÃo de referÃncia com a metodologia de Penman-Monteith/FAO, podem ser utilizadas todas as equaÃÃes de temperatura mÃdia diÃria do ar apresentadas, pois o coeficiente de determinaÃÃo (RÂ) apresentou os seguintes valores quando correlacionados com o cÃlculo de ETo utilizando a mÃdia obtida pela estaÃÃo meteorolÃgica automatizada: mÃtodo FAO (0,9892), mÃtodo INMET (0,9886) e mÃtodo SMESP (0,9888). As equaÃÃes de cÃlculo da umidade relativa do ar, tambÃm, apresentaram o mesmo comportamento, sendo todas as viÃveis, obtendo-se os seguintes valores de RÂ: mÃtodo FAO (0,9972), mÃtodo INMET (0,9980) e mÃtodo SMESP (0,9817). Na estimativa do saldo de radiaÃÃo, influenciado pelas equaÃÃes apresentadas de radiaÃÃo solar global, o mÃtodo de Aguiar et al. (R = 0,3704) apresentou a maior correlaÃÃo de metodologia quando comparada com o mÃtodo da FAO. / The study was carried out in the experimental area of agribusiness Figood-ProduÃÃo de Produtos AgrÃcolas Ltda. in the Distrito de IrrigaÃÃo (irrigation district) Jaguaribe - Apodi (DIJA), Limoeiro do Norte, CearÃ, Brazil, from July to December 2008 and consisted of two experiments. In the experiment I, the treatments consisted of five irrigation frequencies: F1 - total irrigation depth applied in the morning, F2â total irrigation depth applied in the afternoon, F3 -50% of the irrigation depth applied in the morning and 50% applied in the afternoon, F4-1/3 of the irrigation depth applied in the morning, 1/3 of the irrigation depth applied at noon, 1/3 of the irrigation depth applied in the afternoon; F5 -accumulated total irrigation depth applied every two days. In the experiment II, the treatments were five different doses of nitrogen fertilizer (50, 75, 100, 125 and 150% of the standard nitrogen fertilizer recommendation) applied by fertigation, defined based in the recommended farm's fertilizer amount (from soil analysis ). The experimental design (for both experiments) was organized in randomized blocks with five treatments and four replications. In both experiments, we evaluated the following variables: plant height, plant yield, stem diameter, branch length, number of fruits per plant, fruit diameter, and fruit weight. In the first experiment the average fruit weight ranged between 52.81 and 59.05 g. In experiment II, the average fruit weight ranged between 40.00 and 63.50 g. It was also observed a direct relationship between the number of fruits per plant and plant yield. The different irrigation frequencies did not statistically influence (p > 0.05) plant height, plant yield, stem diameter, branch length, number of fruits per plant, fruit diameter, and fruit weight in the period from 0 to 190 days after the first production pruning of the fig tree cv. Roxo Valinhos. The different levels of nitrogen fertigation applied in the period from 0 to 190 days after the first production pruning of the Roxo Valinhos fig tree did not statistically influence (p > 0.05) plant height, plant yield, stem diameter, branch length, number of fruits per plant, fruit diameter, and fruit weight.

A field experiment was conducted at Inselele, KwaZulu-Natal South Coast, South Africa, in 1999/2000, to assess the influence of shading as related to varying levels of incident solar irradiance as well as microclimatic variations on banana (Musa spp) growth, phenology trends, morphology and productivity. The trial was established in August 1999 on a ratoon plantation. The experimental site, 0.655 ha in extent, comprised of three replications with four treatments having varying levels of incident solar irradiance levels of 100 %, 70 %, 40 % and 20 % under a planting density of 1666 plants ha. The irradiance levels were derived from black shade cloth, erected 1 m above the banana canopy level. Banana plantations have vegetation that does not completely cover the underlying ground surface. For such a canopy, there are basically two distinct and interacting surface components, the overstorey/canopy and the understorey/soil. Independent investigations and measurements of the solar energy fluxes for each of these two components forms a vital step to comprehend the factors that control the overhead energy fluxes in the plantation. In this study, evaluation of flux components in the understorey of the canopy using plastic microlysimeters was conducted. Considering normal variations in field measurements, the agreement and consistency among the different measurements with previous findings was adequate. Microlysimeter measurements of daytime soil evaporation were generally less than 1 mm, with an average of 0.45 mm. The study did not include flux measurement at the two level approach (Bowen ratio and Eddy correlation methods) which could have been compared with the single level approach and microlysimeter results. Two methods of deriving/measurement of energy fluxes were used and the differences between them are discussed. A reassessment of the microlysimeter technique is suggested. For the understorey, the sum of sensible and latent heat fluxes derived from spreadsheet computation was equal to the available energy. Mean soil temperatures at a depth of 20 to 60 mm ranged between 13 and 16 degrees C. This study illustrated that energy flux measurement and interpretation in within the experimental area. Good agreement was found in the patterns of wind speed profile measurements, with the 1.5 m profile depicting a mean difference of 52 % compared to the 3 m profile between day of year (DOY) 230 and 248 inclusive. This suggested that wind speed attenuation was strongly correlated to increment in height within the plantation due to canopy roughness. Differential canopy temperatures (measured with infrared thermometry) were more sensitive to the vapour pressure deficit than to wind speed. The most dense shade affected banana productivity indicated by a bunch weight of 22.69 and 33.65 kg under the 20 % and 100% irradiance treatments respectively. The bunch mass reduction was 32 %. Flowering dates were delayed by 8 days, 13 days and 21 days with incident irradiances of 70 %, 40 % and 20 % of the unshaded control (lOO % irradiance), respectively. The phenological responses in this study appear to be a result ofa contribution or interaction of both seasonal responses and shading treatments and this is further evidenced by the high levels of correlation (98.4%) reported between these two variables. Shading resulted in diminished leaf emergence rates (LER), pseudostem circumference and pseudostem height. However, just before flowering, no significant differences were observed in the pseudostem circumferences. There was a progressive increment in pseudostem height for all the treatments, with the 20 % irradiance treatment depicting the least heights registered compared to the rest of the treatments. Evident seasonal differences were registered in the LER and emergence to harvest (EH) interval. Comparison of LER and mean air temperature trends revealed a similar curve pattern and depicted a highly positive correlation of90.4%. The highest LER of3.8 was recorded in the month of January at a peak mean monthly air temperature of24.3°C while the lowest LER of 1.2 was registered in July which had the lowest mean air temperature of 14.7 cc. The EH intervals measured between September and December flowering varied from 125 days to 112 days respectively before harvest yet the April to May flowering had an EH duration of186 to 195 days respectively. The strong seasonal influence on phenological responses is further confirmed by these EH trends. Reductions in LAI observed with time were principally due to leaf senescence. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2001.

Limited water supplies are available to satisfy the increasing demands of crop production. It is therefore very important to conserve the water, which comes as rainfall, and water, which is used in irrigation. A proper irrigation water management system requires accurate, simple, automated, non-destructive method to schedule irrigations. Utilization of infrared thermometry to assess plant water stress provides a rapid, nondestructive, reliable estimate of plant water status which would be amenable to larger scale applications and would over-reach some of the sampling problems associated with point measurements. Several indices have been developed to time irrigation. The most useful is the crop water stress index (CWSI), which normalizes canopy to aIr temperature differential measurements, to atmospheric water vapour pressure deficit. A field experiment was conducted at Cedara, KwaZulu-Natal, South Africa, to determine the non-water-stressed baselines, and CWSI of cereal rye (Secale cereale L.) from 22 July to 26 September 2002, and aImual (Italian) ryegrass (Lolium multiflorum Lam.) from October 8 to December 4, 2002, when the crops completely covered the soil. An accurate measurement of canopy to air temperature differential is crucial for the determination of CWSI using the empirical (Idso et al., 1981) and theoretical (Jackson et al., 1981) methods. Calibrations of infrared thermometers, a Vaisala CS500 air temperature and relative humidity sensor and thermocouples were performed, and the reliability of the measured weather data were analysed. The Everest and Apogee infrared thermometers require correction for temperatures less than 15 QC and greater than 35 QC. Although the calibration relationships were highly linearly significant the slopes and intercepts should be corrected for greater accuracy. Since the slopes of the thermocouples and Vaisala CS500 air temperature sensor were statistically different from 1, multipliers were used to correct the readings. The relative humidity sensor needs to be calibrated for RH values less than 25 % and greater than 75 %. The integrity of weather data showed that solar irradiance, net irradiance, wind speed and vapour pressure deficit were measured accurately. Calculated soil heat flux was underestimated and the calculated surface temperature was underestimated for most of the experimental period compared to measured canopy temperature. The CWSI was determined using the empirical and theoretical methods. An investigation was made to determine if the CWSI could be used to schedule irrigation in cereal rye and annual rye grass to prevent water stress. Both the empirical and theoretical methods require an estimate or measurement of the canopy to air temperature differential, the non-waterstressed baseline, and the non-transpiring canopy to air temperature differential. The upper (stressed) and lower (non- stressed) baselines were calculated to quantify and monitor crop water stress for cereal rye and annual ryegrass. The non-water-stressed baselines were described by the linear equations Te - Ta = 2.0404 - 2.0424 * VPD for cereal rye and Te - Ta = 2.7377 - 1.2524 * VP D for annual ryegrass. The theoretical CWSI was greater than the empirical CWSI for most of the experimental days for both cereal rye and annual ryegrass. Variability of empirical (CWSI)E and theoretical (CWSI)T values followed soil water content as would be expected. The CWSI values responded predictably to rainfall and irrigation. CWSI values of 0.24 for cereal rye and 0.29 for annual ryegrass were found from this study, which can be used for timing irrigations to alleviate water stress and avoid excess irrigation water. The non-water-stressed baseline can also be used alone if the aim of the irrigator is to obtain maximum yields. However the non-water-stressed baseline determined using the empirical method cannot be applied to another location and is only valid for clear sky conditions. And the non-water-stressed baseline determined using theoretical method requires computation of aerodynamic resistance and canopy resistances, as the knowledge of canopy resistance, however the values it can assume throughout the day is still scarce. The baseline was then determined using a new method by Alves and Pereira (2000), which overcomes these problems. This method evaluated the infrared surface temperature as a wet bulb temperature for cereal rye and annual ryegrass. From this study, it is concluded that the infrared surface temperature of fully irrigated cereal rye and annual ryegrass can be regarded as a surface wet bulb temperature. The value of infrared surface temperature can be computed from measured or estimated values of net irradiance, aerodynamic resistance and air temperature. The non-water-stressed baseline is a useful concept that can effectively guide the irrigator to obtain maximum yields and to schedule irrigation. Surface temperature can be used to monitor the crop water status at any time of the day even on cloudy days, which may greatly ease the task of the irrigator. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2003

Theory predicts that the time dependent voltage curve of a thermocouple psychrometer where there is no change in output voltage with time during the evaporation cycle defines the wet bulb temperature T[w] corresponding to the water potential. In practice, a change in voltage with time does occur and it is convenient to define the voltage corresponding to the water potential as the maximum point of- inflection voltage. A predictive model based on calibration data at a few tempertures is used to obtain the psychrometer calibration slope at any temperature. Use of this model indicates that psychrometers differ from each other and therefore must be individually calibrated if accuracy better than ±5 % in the measurement of water potential is required. Dewpoint hygrometers are shown to be less temperature sensitive than psychrometers and have the added advantage of a voltage sensitivity nearly twice that of psychrometers, typically -7,0 x 10¯³ μV/kPa compared to -3,7 x 10¯³ μV/kPa at 25 °C. The accurate temperature correction of hygrometer calibration curve slopes is a necessity if field measurements are undertaken using either psychrometric or dewpoint techniques. In the case of thermocouple psychrometers, two temperature correction models are proposed, each based on measurement of the thermojunction radius and calculation of the theoretical voltage sensitivity to changes in water potential. The first model relies on calibration at a single temperature and the second at two temperatures. Both these models were more accurate than the temperature correction models currently in use for four leaf psychrometers calibrated over a range of temperatures (15 to 38°C). The model based on calibration at two temperatures is superior to that based on only one calibration. The model proposed for dewpoint hygrometers is similar to that for psychrometers. It is based on the theoretical voltage sensitivity to changes in water potential. Comparison with empirical data from three dewpoint hygrometers calibrated at four different temperatures indicates that these instruments need only be calibrated at, say 25°C, if the calibration slopes are corrected for temperature. A model is presented for the calculation of the error in measured thermocouple hygrometric water potential for individual hygrometers used in the dewpoint or psychrometric mode. The model is based on calculation of the relative standard error in measured thermocouple psychrometric water potential as a function of temperature. Sources of error in the psychrometric mode were in calibration of the instrument as a function of water potential and temperature and in voltage (due to electronic noise and zero offsets) and temperature measurement in the field. Total error increased as temperature decreased, approaching a value usually determined by the shape of the thermocouple junction, electronic noise (at low voltages less than 1 μV) and errors in temperature measurement. At higher temperatures, error was a combination of calibration errors, electronic noise and zero offset voltage. Field calibration data for a number of leaf psychrometers contained total errors that ranged between 6 (at a °C) and 2 %(at 45 °C) for the better psychrometers and between 11 (at 0° C) and 5 % (at 45 C) for the worst assuming that the zero offset was 0,5 μV. Zero offset values were less than 0,7 μV at all times. The dewpoint errors arose from calibration of the dewpoint hygrometer as a function of water potential, extrapolation of the calibration slope to other temperatures, setting the dewpoint coefficient and errors in voltage and temperature measurement. The total error also increased as temperature decreased, because of the differences in temperature sensitivity between dewpoint and psychrometric calibration constants. Consequently, the major source of error in the dewpoint mode arose from the difficulty in determining the dewpoint coefficient. This error, which is temperature dependent, contains three subcomponent errors; the temperature dependence, random variation associated with determining the temperature dependence and error in setting the correct value. Calibration and extrapolation errors were smaller than those of the psychrometric technique. Typically, the error in a dewpoint measurement varied between about 6 and 2 % for the best hygrometer and between 10 and 3 % for the worst for temperatures between 0 and 45 °C respectively. At low temperatures, the dewpoint technique often has no advantage over the psychrometric technique, in terms of measurement errors. In a comparative laboratory study, leaf water potentials were measured using the Scholander pressure chamber, psychrometers and hydraulic press. Newly mature trifoliates cut from field grown soybean (Glycine max (L) Merr. cv. Dribi) were turgidified and, after different degrees of dehydration, leaf water potential measured. One leaflet from the trifoliate was used for the thermocouple psychrometer and another for the press while the central leaflet with its petiolule was retained for use in the pressure chamber. Significant correlations between measurements using these instruments were obtained but the slopes for hydraulic press vs psychrometer measurement curve and hydraulic press vs pressure chamber were 0,742 and 0,775 respectively. Plots of pressure-volume curves indicate that the point of incipient plasmolysis was the same (statistically) for the thermocouple psychrometer and the pressure chamber, but much larger for the hydnaulic press. The above-mentioned differences between the three instruments emphasize the need for calib rating the endpoint defined us i ng the press against one or more of the standard techniques, and, limi ting the use of the press to one person. Cuticular resistance to water vapour diffusion between the substomatal cavity and the sensing psychrometer junction is a problem unique to leaf psychrometry and dewpoint hygrometry; this resistance is not encountered in soil or solution psychrometry. The cuticular resistance may introduce error in the leaf water potential measurement. The effect of abraiding the cuticle of Citrus jambhiri to reduce its resistance, on the measured leaf water potential was investigated. Psychrometric measurements of leaf water potential were compared with simultaneous measurements on nearby leaves using the Scholander pressure chamber, in a field situation. Leaf surface damage, due to abrasion, was investigated using scanning electron microscopy. Thermocouple psychrometers are the only instruments which can measure the in situ water potential of intact leaves, and which may be suitable for continuous, non-destructive monitoring of water potential. Unfortunately, their usefulness is limited by a number of difficulties, among them fluctuating temperatures and temperature gradients within the psychrometer, sealing of the psychrometer chamber to the leaf, shading of the leaf by the psychrometer and resistance to water vapour diffusion by the cuticle when the stomates are closed. Using Citrus jambhiri, several psychrometer designs and operational modifications were tested. In situ psychrometric measurements compared favourably with simultaneous Scholander pressure chamber measurements on neighbouring leaves, corrected for the osmotic potential and the apparent effect of "xylem tension relaxation" following petiole excision. It is generally assumed that enclosure of a leaf by an in situ thermocouple psychrometer substantially modifies the leaf environment, possibly altering leaf water potential, the quantity to be measured. Furthermore, the time response of leaf psychrometers to sudden leaf water potential changes has not been tested under field conditions. In a laboratory investigation, we found good linear correlation between in situ leaf psychrometer (sealed over abraided area) and Scholander pressure chamber measurements (using adjacent leaves) of leaf water potential, 2 to 200 minutes after excision of citrus leaves. A field investigation involved psychrometric measurement prior to petiole excision, and 1 min after excision, simultaneous pressure chamber measurements on adjacent citrus leaves immediately prior to the time of excision and then on the psychrometer leaf about 2 min after excision. Statistical comparisons indicated that within the first two minutes after excision, psychrometer measurements compared favourably with pressure chamber measurements. There was no evidence for a psychrometer leaf water potential time lag. For the high evaporative demand conditions, water potential decreased after excision by as much as 700 kPa in the first minute. Psychrometer field measurements indicated that within the first 5 min of leaf petiole excision, the decrease in leaf water potential with time was linear but that within the first 15 s, there was a temporary increase of the order of a few tens of kilopascal. The thermocouple psychrometer can be used to measure dynamic changes in leaf water potential non-destructively, with an accuracy that compares favourably with that of the pressure chamber. Using in situ thermocouple leaf hygrometers (dewpoint and psychrometric techniques employed) attached to Citrus jambhiri leaves, an increase in measured water potential immediately following petiole excision was observed. The increase ranged between 20 to 80 kPa and occurred 30 s after petiole excision and 100 s after midrib excisions. No relationship between the actual leaf water potential and the increase in water potential due to excision, was found. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1982.